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We narrowed to 407 results for: expression vector

Showing: 321 - 340 of 407 results

  1. Viral Vectors

    Type
    Collection
    ...Biosafety Guide Blog: Plasmids 101-Viral Vector Elements Viral Vectors 101 eBook Overview of Viral Plasmids... Lab Lentivector Toolbox Guide to Generating Recombinant Adenoviruses Eric Campeau's Viral Vector Guide...List of viral vector plasmids available at Addgene and other related resources. Special...cells. These viruses enable long-term stable gene expression and are permanently integrated into the host ... These viruses can direct long-term transgene expression, but generally are not permanently integrated...efficiency and direct high but transient transgene expression. γ-Retrovirus Plasmids | Guide γ-retroviruses...the host genome, enabling long term transgene expression. Overview of Viral Plasmids While plasmids are...

  2. Retrograde AAV viral preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Retrograde AAV Viral Vector Packaging Service: Retrograde...Color-flipping switch. Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP in Cre-positive...GFP-tagged Cre expression Recombinases Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn Cre expression Recombinases...CamKII Cre expression Recombinases Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression with simultaneous...simultaneous dTomato expression Recombinases Larsen 107788 AAV.rTH.PI.Cre.SV40 rTH Cre expression Recombinases...physically-separate mCherry expression Recombinases Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases ...pAAV-EF1a-Flpo EF1a Flpo expression Recombinases Deisseroth 55638 pAAV-EF1a-vCre EF1a VCre expression Recombinases...

  3. Plan Your Experiment

    Type
    Collection
    ...Delivery method Expression System Components of System Application Mammalian expression vector Cas enzyme ...system. Select Expression System To use CRISPR, you will need both Cas9 and a gRNA expressed in your target...to the transient Cas9 expression. The table below summarizes the major expression systems and variables...details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or gRNA in a mammalian... in a single lentiviral transfer vector or separate transfer vectors May contain reporter gene (e.g. GFP... or electroporation Transient expression of CRISPR components Expression decreases as RNA is degraded ...gRNA and/or Cas9 expression or (2) a lack of efficient target cleavage in cells expressing both Cas9 and ...

  4. Zebrafish Plasmid Collection

    Type
    Collection
    .... pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS),...fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed. EXPRESs...in zebrafish. Zebrafish expression atlas - A zebrafish-specific gene expression database that provides ...fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large collections or kits are described...CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples...found below: UFlip CRISPR targeted integration vectors for cell-type specific conditional gene inactivation...inactivation - Jeffrey Essner Lab GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner...

  5. CRISPR Plasmids - Tagging

    Type
    Collection
    ...targeting vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general... one vector from the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which expresses an untagged...gene-specific CRISPR and donor vectors as well as pX330S-2-PITCh, expressing a generic gRNA targeting the...Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific...are available. An improved FLP recombinase expression vector allows for efficient marker cassette FLP-out...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...has been adapted to allow tagging of proteins expressed from their natural chromosomal context. These ...

  6. Microbiology Resources

    Type
    Collection
    ...Barbier Lab Cloning and Expression Bacteria and yeast expression vectors : FX cloning System - Geertsma and... E. coli expression - Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier...- Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers, fusion proteins, etc - Lindquist...Sets of plasmids for fluorescence, cloning and expression, and synthetic biology. CRISPR Resources : Find...plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied as one item. ...Santalucia Labs pCri System : Heterologous protein expression in E. coli, B. subtilis and P. pastoris - Gomis-Rüth...constructs - Lahaye Lab Vaccinia virus ORF library : Expression library that contains each of the predicted open...

  7. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...Publication Empty gRNA Expression Vectors You can use the tables on Addgene's Empty gRNA Vectors page to search... CRISPR Mammalian Expression CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...plasmids have been designed for use in mammalian expression systems. Cut Fully functional CRISPR/Cas enzymes...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...

  8. Lentiviral Prep Service

    Type
    Collection
    ...more details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled...4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau Viral Service...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently ... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus...52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused ...61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A GFP Zhang 61425 lenti...

  9. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Insert Vector Type Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  10. Worm Expression Resources

    Type
    Collection
    ...General Tools Fire Lab C. elegans Vector Kit - Andrew Fire Lab. A set of vectors for C. elegans research, including...and spatial control of gene expression in C. elegans by combining expression of the FLP recombinase from...can knock down genes by feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily...developed and characterized which allow researchers to express desired constructs in precise cell types and/or...empty backbones, fluorescent proteins, and Cre expression plasmids. Large collections or kits are described...ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids...plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied as one item. ...

  11. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    .... PubMed Central PMCID: PMC4523203. Many empty vectors containing these fluorescent proteins are available... endogenous state that you want to measure (overexpressing a gene or protein after transfection). Future...

  12. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...break (DSBs) at a specific location based on a co-expressed gRNA-defined target sequence. DSBs are preferentially...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  13. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  14. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ... 101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone for bacterial expression under T7 control 101135...pSNAP-tag (m) Vector SNAP-tag Empty backbone for stable and transient mammalian expression 101133 pSNAP-CaaX...pCLIP-tag (m) Vector CLIP-tag Empty backbone for stable and transient mammalian expression 101134 pCLIPf-H2B...101127 pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag ...pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-GPI...Synthases (AcpS and Sfp, respectively) may be expressed and purified as described previously in Stachelhaus...

  15. Depositor Collections

    Type
    Collection
    ...research, visualization, protein expression, synthetic biology, and viral vector plasmids....to drive selective expression in the brain - Simpson Reagents for improved expression of recombinant phosphoproteins...Plasmids Terminator Plasmids Viral Vectors pLEG/pREG Modular Viral Vector System - Dankort...Collection Botman-Teusink Yeast FP Collection Protein Expression Cell Migration Consortium Malate Dehydrogenase...Open Enzyme Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection Synthetic...

  16. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...cleavage, Bac-to-Bac Expression System, AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins...TEV cleavage, AmpR 26095 pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine...SGC Toronto SGC Oxford SGC Stockholm Bacterial Expression Plasmids (empty backbones/controls): ID Plasmid...Thrombin cleavage, Bac-to-Bac Baculovirus Expresion vector 26117 pNIC-CH EF199843 Hexahistidine tag, AmpR ...hexahistidine tag and TEV cleavage, Bac-to-Bac Expression System, AmpR Related Resources SGC: http://www.thesgc.org...

  17. AAV for Neuronal Tracing

    Type
    Collection
    ... Viral Vector Packaging Service AAV Monosynaptic Neuronal Tracing Viral Vector Packaging Service... Addgene does not distribute rabies virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description...direct virus infection specifically into cells that express the TVA viral receptor. Thus, by pseudotyping the...virus with EnvA, only neuronal cells engineered to express TVA will be infected. Schematic depicting steps...to the starter cell. (2) The starter cell now expresses TVA and G-protein. EnvA-pseudotyped RAVB is delivered... via G-protein receptors, which are naturally expressed at presynaptic nerve terminals. (4) Neurons projecting...G-protein coated RABV. These projection neurons do not express G-protein, and thus do not give rise to G-protein...

  18. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...

  19. Caltech Systemic Capsids

    Type
    Collection
    ... Viral Vector Packaging Service AAV Caltech Systemic Capsids Viral Vector Packaging Service... a new window) . Viral Vectors 101: Systemic Capsids PHP.eB These viral vector preparations were produced...pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn Cre-EGFP expression Cre Wilson 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP Cre-expression Cre Wilson 107738...CN4825) minBG iCre(R297T) expression iCre AIBS , Ting PHP.S These viral vector preparations were produced... CAP-B10 CAP-B22 AAV9-X1.1 As part of our Viral Vector Packaging Service , Addgene is distributing ready-to-use...of our standard viral production , these viral vectors undergo quality control, including AAV titering...testing, and full sequencing of the final viral vector preparation. Systemic Delivery AAV Capsid Choice...

  20. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...
Showing: 321 - 340 of 407 results