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We narrowed to 660 results for: otos

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Showing: 321 - 340 of 660 results

  2. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...virus properly and be consistent when following protocols. Need Virus? Check out Addgene's New Viral Service...pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009): 495-505. PubMed PMID: 19300443. 7....

  4. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...correct? Is the purified plasmid DNA viable? Or cytotoxic? How and where did things go wrong? The use of...reagents or the transfection process itself has any cytotoxic effects on the target cells. Another type of transfection...

  5. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...Secreted Proteins: The most common purification protocols are designed for soluble, cystosolic protein products... can be recovered and purified using a special protocol. Alternatively, reducing the concentration of ...

  6. March for Science

    Type
    Blog Post
    ...Stephanie Hays, a scientist with a passion for photosynthetic communities, microbial interactions, and science...University. She is a scientist with a passion for photosynthetic communities, microbial interactions, and science...

  7. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...activity. Researchers have designed a variety of photoswitchable elements into many other types of proteins.... 30x SuperPLDs avoid dark-state activity and cytotoxicity better than the 50x and 100x variants. But those...

  8. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...Lentiviral Guide and our lentiviral production protocol.  One thing to keep in mind is that titering these... these IDLVs may require a slightly different protocol than regular lentiviral vectors. This is because...

  10. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...ScFvs can also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer, autoimmune...facilitates signal transduction and release of cytotoxic granules from the T cell. When a CAR has an scFv...

  11. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...the application of this approach is limited to protoplast cells (plant cells whose cell wall has been removed...recommend reading the associated publications or protocols to get the most out of the plasmid you chose for...

  12. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...infection. Dapeng Zhou Other Resources Protocols Bloom lab - Protocol and Reagents for Pseudotyping Lentiviral... Spike Protein Nemazee lab - Pseudotyped virus protocol for coronaviruses (DOCX, 184 KB) Addgene Blog ...

  13. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...Find other tips and tricks for molecular biology protocols Browse other articles related to antibodies Resources...Resources on Addgene.org Find written and video protocols Visit Addgene's antibodies collection ...

  14. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ... Require Ex Vivo Handling of Embryos. Current Protocols in Human Genetics 88: . https://doi.org/10.1002... CRISPR‐Cas9‐Mediated Genome Editing. Current Protocols in Mouse Biology 6:39–66 . https://doi.org/10.1002...

  15. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...open-source' protocol for making customized zinc-finger arrays. Maeder ML et al. Nat Protoc. . 2009. Sept...customized zinc-finger nucleases. Foley JE et al. Nat Protoc. . 2009. Dec 3. 4(12):1855-1867. PubMed PMID 20010934...

  16. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...basic-hFX-promotor(1kb) FXN hFXN Friedreich ataxia Michael Ristow 14979 pGL3-basic-hFX-promotor(1,3kb) FXN...basic-hFX-promotor(2,5kb) FXN hFXN Friedreich ataxia Michael Ristow 14981 pGL3-basic-hFX-promotor(2kb, distal... DCTN1 CMV ALS, motor neuron disease Trina Schroer 51221 CMV-CC2 DCTN1 CMV ALS, motor neuron disease Trina... ALS, motor neuron disease Caren Norden 105971 pME mKate2-dynactin1-811 DCTN1 mKate2 ALS, motor neuron...Aebischer 36154 pEGFP-p150Glued DCTN1 GFP CMV ALS, motor neuron disease David Stephens 36382 pSMP-DNMT1_1... Trina Schroer 51412 CMV-p150 DCTN1 CMV ALS, motor neuron disease Trina Schroer 51435 pGL3Basic_ME.1/ApoEpromoter...Charlet-Berguerand 74170 pVEX-CC1 DCTN1 tac ALS, motor neuron disease Trina Schroer 75437 p-AAV-sh[SNCA...

  17. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ... a new window) . Protocols The Allen Institute for Cell Science has several protocol videos available ...Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute for Cell Science has produced...

  18. AAV Packaged on Request

    Type
    Collection
    ... your AAV experiments, remember to share your protocols and results through Addgene’s Data Hub . By contributing...globe and shared our well-developed and trusted protocols and peer-reviewed techniques (Link opens in a ...preps. Data Hub Access Addgene’s Data Hub for protocols and results shared by other scientists using AAV...

  20. Important Considerations When Using AAVs

    Type
    Blog Post
    ...Lung AAV4, AAV5, AAV6, AAV9 Pancreas AAV8 Photoreceptor Cells AAV2, AAV5, AAV8 RPE (Retinal Pigment...Activated By Designer Drugs) to determine the role of serotonin in movement. Follow her on twitter @katrinaboards...
Showing: 321 - 340 of 660 results