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We narrowed to 658 results for: otos

Showing: 321 - 340 of 658 results

  3. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... for Protein Expression in Multiple Hosts. Curr Protoc Protein Sci. 2003 Feb;Chapter 5:Unit 5.17. PubMed...Resources on Addgene.org Find Plasmid Cloning Protocols Watch Our Diagnostic Restriction Digest Video ...

  4. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...-I3, while initially dimmer, showed greater photostability and also matured considerably faster, with ...abscessus, an increasingly common human pathogen notorious for its high resistance to antibiotics. Several...

  5. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...) the sequence is located just upstream of a Protospacer Adjacent Motif (PAM sequence). The 3-5 nucleotide...edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target...

  6. Hot Plasmids - November 2023

    Type
    Blog Post
    ...antibody new tricks By Ashley Waldron While IHC protocols typically call for thin sections of tissue, the...perform well in standard IHC and western blot protocols. You can find more specific information on the...

  8. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...virus properly and be consistent when following protocols. Need Virus? Check out Addgene's New Viral Service...pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009): 495-505. PubMed PMID: 19300443. 7....

  10. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...correct? Is the purified plasmid DNA viable? Or cytotoxic? How and where did things go wrong? The use of...reagents or the transfection process itself has any cytotoxic effects on the target cells. Another type of transfection...

  11. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...Secreted Proteins: The most common purification protocols are designed for soluble, cystosolic protein products... can be recovered and purified using a special protocol. Alternatively, reducing the concentration of ...

  12. Fluorescent Proteins: FRET

    Type
    Collection
    ...excitation of the fluorophore and emission of a photon) of the donor can be measured using fluorescence... standards Thaler et al. (2005) Quantitative multiphoton spectral imaging and its use for measuring resonance...standards and fusion proteins Feldmann et al. (2023) Protocol for deriving proximity, affinity, and stoichiometry...

  13. Antibody Guide

    Type
    Collection
    ...applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification Methods...binding between the protein and DNA. Some ChIP protocols use enzyme digestion instead of sonication. This...Special considerations Many of the steps in this protocol vary depending on assay and microscopy techniques... may be a fluorophore or an enzyme. The basic protocols and controls follow that of IF (above). Sample...

  14. March for Science

    Type
    Blog Post
    ...Stephanie Hays, a scientist with a passion for photosynthetic communities, microbial interactions, and science...University. She is a scientist with a passion for photosynthetic communities, microbial interactions, and science...

  15. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...ScFvs can also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer, autoimmune...facilitates signal transduction and release of cytotoxic granules from the T cell. When a CAR has an scFv...

  16. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...activity. Researchers have designed a variety of photoswitchable elements into many other types of proteins.... 30x SuperPLDs avoid dark-state activity and cytotoxicity better than the 50x and 100x variants. But those...

  17. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...Lentiviral Guide and our lentiviral production protocol.  One thing to keep in mind is that titering these... these IDLVs may require a slightly different protocol than regular lentiviral vectors. This is because...

  19. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...the application of this approach is limited to protoplast cells (plant cells whose cell wall has been removed...recommend reading the associated publications or protocols to get the most out of the plasmid you chose for...

  20. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...Find other tips and tricks for molecular biology protocols Browse other articles related to antibodies Resources...Resources on Addgene.org Find written and video protocols Visit Addgene's antibodies collection ...
Showing: 321 - 340 of 658 results