We narrowed to 883 results for: prot

...consist of a VSD fused to a single fluorescent protein (FP). This class of GEVI was the earliest developed...that of the VSD GEVIs. However, since rhodopsin proteins were initially evolved for ion transport, not ...viruses also have various serotypes – the surface proteins that confer affinity for a target – and this serotype...pseudotyping is also a powerful tool that swaps capsid proteins from different viruses to optimize them for targeting...Addgene.org Addgene’s Viral Services Addgene’s Virus Protocols Addgene’s Chemogenetics Guide Resources on the... Viral Vectors 101: Optogenetics Fluorescent Proteins 101: Introduction to FRET ...

... of a fusion protein: Histidine tag, malate dehydrogenase, and green fluorescent protein. Biochemistry... graduate school. As a stable, easily purified protein with easily measured activity, MDH is particularly...structure, function, and interaction with other proteins.   It is important to note that research ...acknowledging student apprehensions about “failure” (protocols not working, hypotheses being rejected, etc.),..., Mukherjee, S., Uzzi, B. 2020. Mentorship and protégé success in STEM fields. Proceedings of the National...

...guidelines here. A detailed transformation protocol, as well as protocols for growing Arabidopsis are also available...transcriptional and translational dynamics of genes and proteins in living plants, to complementing mutant phenotypes...and generating N and C terminally tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, etc.) or...more than one construct at a time. For detailed protocols, volumes, spin speeds, recipes, and Agrobacterium...your insertion in the absence of selection. For a protocol for antibiotic selection, check out Harrison et...

...able to come of age. The in vivo assembly cloning protocol In Vivo Assembly (IVA) cloning (Garcia-Nafria ... to be performed using a simple two-step, 2 hr protocol prior to transformation (Figure 1). As this approach...procedures are performed using the same three step protocol:  Fragment generation - Any linear DNA fragments...epitope tag can be moved to the N-terminus of a protein-coding sequence, or a mutation can be introduced...plasmid cloning Resources on Addgene.org Read our protocols for plasmid cloning Find our molecular biology...biology reference here Find molecular biology protocols here  ...

...recognition sequences of an exogenous DNA-binding protein such as LexA into the target locus, which is the... DNA-binding molecule technologies such as TAL proteins and CRISPR became available, I was very excited...easily isolated for identification of associated proteins, RNAs, and other genomic regions. I believe that...analyze signal-induced nuclear translocation of proteins in 2004. In addition, we have recently developed...Addgene Alerts box. References Identification of Proteins Associated with an IFNgamma-Responsive Promoter...genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin...

...have a natural preference to stay in dark areas (protected) as opposed to brightly illuminated ones (risky...environments by initially moving along a wall (protected) rather than being out in the open (risky). Taking...comprised of a brightly lit (risky) and a dark (protected) area. When measuring the time spent in a particular...derived neurotrophic factor (BDNF). BDNF is a small protein involved in neurogenesis, neuronal survival, and...Channelrhodopsin 2 (ChR2), a modified receptor protein that activates neurons upon light stimulation. ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

...crisis. I like how they are using protocols.io to create a protocol community! Asclepias syriaca and Asclepias...solicitation requires funded labs to rapidly disseminate protocols, methods, data, and tools that result from their...manipulated by injected RNA and can express fluorescent proteins. This lab has created an automated tank for feeding... fusion is such that the two plants share RNA, proteins, and DNA. Euprymna scolopes (Joshua Rosenthal...the brain allows the squid to create a different protein. Did you know that most cephalopods die after they...

... target is present immediately upstream of a Protospacer Adjacent Motif (PAM), the sequence NGG.  If these...site e.g., deleting the catalytic residue of a protein or knocking out a very small peptide. In these ... within the editing window (bases 4-8 of the protospacer). While this is a small window, this system can... can be used with a variety of impaired Cas9 proteins (such as SpRY) with altered PAM requirements, giving...epitope tagged dCas9 and antibody-activator fusion proteins. The Synergistic Activation Mediator complex, ... target sequence has been modified!  The exact protocol may vary depending on your specific experiment...

...major structural protein GvpA, which seemed overexpressed compared to accessory proteins. Excess gvpA plasmid...of ARGs results in the production of air-filled protein nanostructures called gas vesicles, which scatter.... coli. We deleted an unannotated hypothetical protein, improving expression, and named this gene set ...of toxicity or burden relative to a fluorescent protein control. We also added the toxin-antitoxin stability...in mammalian cells without the shell stiffening protein GvpC, which allowed the GVs to be flexible and ...

...antibiotics also block protein synthesis, and the neomycin resistance gene protects against any of them....and eukaryotes. For example, puromycin inhibits protein synthesis across kingdoms by targeting a shared...A puromycin resistance gene on your vector can protect both plasmid-carrying bacteria and eukaryotes. ... vector with a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters might...production, you probably don’t need it to express your protein of interest. However, you do still need a species-compatible...

...that the protocol is not approved for clinical use at this point, they hope that the protocol serves as...few years, scientists have utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a, to develop simple,...has adapted natural RNase activity of the Cas13 protein to develop and optimize the method termed Specific...et al., 2018). Now, the Zhang lab has shared a protocol for using SHERLOCK to detect SARS-CoV-2 RNA. The...endoribonuclease activity of the type III-A CRISPR-associated protein Csm6. RNA 22:318–329 . https://doi.org/10.1261/...

...there are many protein-based toxin-antitoxin systems. One of the most well known protein-based toxin-antitoxin...specific sequences in RNA resulting in a blockage of protein synthesis. The antitoxin MazE, which binds and ...inhibits MazF, is continually cleaved by the ClpAP protease so without continual production of MazE, the toxin...Toxin-antitoxin systems are not just restricted to the protein realm. Let’s take a look at a toxin-antitoxin system...additions to constructs that are used to drive high protein expression in vivo. Expression plasmids are often...

...modified reprogramming factor cocktail, and viral protein EBNA-1. Methods for reprogramming hiPSCs from ...EBNA-1 sequence that encodes for the DNA binding protein EBNA-1 [7]. These two sequences are necessary and... of the vectors in human cells [8]. The EBNA-1 protein recognizes OriP and induces amplification of the...transiently. The transiently expressed EBNA-1 increases protein expression from the other episomal plasmids. When...Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by ...combination of activated T cells and Sendai virus. Nat Protoc, 2012. 7(4): p. 718-28. PubMed PMID: 22422317. ...

...the Gateway System for Protein Expression in Multiple Hosts. Curr Protoc Protein Sci. 2003 Feb;Chapter ... during the BP or LR reactions. Since the CcdB protein inhibits the growth of CcdB sensitive E. coli strains...strategy, it’s important to include the proper protein expression elements (ribosome recognition sequences...Resources on Addgene.org Find Plasmid Cloning Protocols Watch Our Diagnostic Restriction Digest Video ...

...triggers. This is done by creating a split dCas9 protein that can reassemble when exposed to specific UV...similar tools were made using other DNA binding proteins such as zinc fingers (ZF) and TAL effectors (TALE...new gRNA rather than engineering an entirely new protein. This makes it much cheaper to use Cas9 activators...CRISPR-based screens can be used to gain insight into protein structure and function (Pan et al., 2018). cDNA... A, Kadoch C (2018) Interrogation of Mammalian Protein Complex Structure, Function, and Membership Using...Gilbert LA, Qi LS, Weissman JS, Vale RD (2014) A Protein-Tagging System for Signal Amplification in Gene...

...for a lentivirus are determined by the envelop protein. Many times lentiviral vectors are engineered with...Lentiviruses pseudotyped with the VSV-G envelope protein use the LDL receptor to infect cells and have a...However, viruses pseudotyped with other envelope proteins may have a limited number of targets and therefore...in the mix Even before the lentiviral envelope protein attaches to cell surface receptors, other important...virus properly and be consistent when following protocols. Need Virus? Check out Addgene's New Viral Service...pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009): 495-505. PubMed PMID: 19300443. 7....

... be used as a proxy measure for differences in protein expression. (If you're just getting started, check...included in commercial sets and most machines have a protocol that automatically performs the normalization ...normalization step.  Machines Most general-use protocols, unless otherwise specified, were likely developed on the...LightCycler, you’ll likely need to re-optimize protocols for that machine. (Teo, et al, 2002; see paper...an individually run sample when developing the protocol and optimized until the multiplexed and individual...samples or sample conditions change, the multiplexed protocol may have to be re-optimized.  While troublesome...eds.), Quantitative Real-Time PCR: Methods and Protocols, Methods in Molecular Biology, vol. 1160, DOI ...

...envelope protein under the control of the powerful mammalian CMV promoter. Envelope proteins are a key...key component of lentiviruses. These proteins coat the virus and help determine the types of cells that... preps. You can find our lentivirus production protocol on our website and tips for titering your lentivirus...check out our lentivirus guide page. For more protocols and information, be sure to check out the Trono...

...at the Institute for Protein Innovation (IPI), a Boston-based, nonprofit protein research institute, partnered...containing ligand-binding heads and long legs that protrude from the cell membrane, physically connecting ...Table 1: IPI antibodies  Antibody Target Proteins Reactivity Isotype Source Species Anti-Integrin...plasmids. To encourage collaboration and education in protein science, IPI hosts events and training courses ...Faulds is a science writer at the Institute for Protein Innovation. She writes about all things IPI, from...

...production standpoint, it wasn’t ideal for our Lab Protocol videos. It’s really nice to have a scientist on-screen... on-screen, somebody who really knows the protocol and can guide viewers through. I know cameras, but ...thankfully volunteered, and we decided to focus on a protocol that didn’t require any lab shooting, just animation...setup for the lab, so we can get back to producing protocol videos?   Figure 4: The remote directing...the pandemic with some incredibly strict safety protocols to keep Addgenies safe. Space was at a premium...why not check out all of the written and video protocols at Addgene? If you need help in the lab, we have...