...Protein A and Protein G are not suitable for all applications, such as protein isolation from serum. In this...capture antibody interaction and/or the downstream application. Typically, if the target protein will be detected...and an isotype control antibody. Alternative applications Oftentimes, an IP-appropriate antibody simply...

...detrimental as too little, resulting in MECP2 duplication syndrome or Rett Syndrome, respectively. One ...editing, MECP2 reactivation, RNA editing, RNA trans-splicing, and protein replacement to reverse the impacts....0.co;2-o Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge O, Zoghbi HY (2013) An AT-Hook Domain in MeCP2...

...affected by many experimental factors. To list some complicating factors for quantifying AAV: qPCR efficiency...8610.4. Hindson CM, Chevillet JR, Briggs HA, Gallichotte EN, Ruf IK, Hindson BJ, Vessella RL, Tewari M... abundant targets: from variable nonsense to publication quality data." Scientific Reports 7 (2017). PubMed...

...strand. While this pathway may not be immediately applicable in DSB-induced genome engineering, it has biological...that can be harnessed by researchers for many applications. Your favorite Cas enzyme, a guide RNA to your...Mouse Geneticist's practical guide to CRISPR applications. Singh P, et al. Genetics 114.169771; (2014)...

...study its function is one of the most popular applications of fluorescent proteins. These fusion proteins...linker sequence short In the early days of GFP applications, many were concerned with steric hindrance by...between secondary structure (beta-sheets or alpha helices). An additional requirement is that the site of...

...genomic DNA in many cell types and often results in biallelic modifications. Most commonly, DSBs are repaired.... Furthermore, CRISPR/Cas9 editing is often bi-allelic, meaning that even if one allele is accurately ...heterozygous mutations by multiplying the single allelic mutation incorporation probabilities our data revealed...

...comparison to a control sample. Southern blotting applications range from confirming the results of cloning...sequence thousands of reads at the same time. Other applications of sequencing, such as confirming the results...replaced these, these techniques still have useful applications and have informed many current techniques. For...

...stimulation. GPCR signaling GPCR signaling is a complicated process involving many downstream signaling mechanisms...break your experiments!  First, closely read the publication, including the supplemental data. You’re most... both of which have extensive collections of publications and information on hardware, optogenetics tools...

...source of piggyBac™ transposase (available from a licensed distributor) allow you to quickly transfer a DNA...piggyBac™ technology can be used for numerous applications, including transgenesis, gene-trap screens, ...technology that may be used for a variety of research applications, including making transgenic cell lines and ...

...vivo animal model should consider the possible elicited immune response from viral infection. Animal safety...rate limiting step and can immediately undergo replication and transcription. However, with scAAV the effective...only can we compare these methods in specific applications, we may also be able to use these comparisons...

...genetic code of living organisms and its societal implications even before the discovery of genes themselves... that revolves around synthetic humans called replicants which are genetically engineered by a powerful...us to always consider the ethics and broader implications of our research.  Want the Genome Editing Trading...

...editing typically needs to be optimized for each application, and the efficacy varies widely — it still almost...editing variants Four years after their initial publication, the Liu lab returned to the reverse transcriptase...Image from Doman et al., 2023, under a CC BY 4.0 license. epegRNAs: Structured RNAs with improved ...

...that the antigen being detected in the intended application — here, flow cytometry — is the desired target...quantification of antibody performance in major research applications. eLife, 12, RP91645. https://doi.org/10.7554...of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. eLife...

...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...or quick refresher on — these common antibody applications.  Thanks for watching, and we hope you found...

...trick. Just flip, and press! Lab tip #2: Tube flick trick When combining reagents in the lab, it is ...the various reagents to a single tube and simply flick the tube several times. This will help ensure ensure...

...from Bruce Wetzel and Harry Schaefer, NCI, NIH | License   I’m updating it regularly as people send me suggestions...synthetic biology, DNA assembly, and various applications. I think these papers are good starting points...

... specificities, from target-specific ones to application-specific ones to anything goes. We've shared ...repositories and search engines Website Targets Application Purpose Notes Antibodypedia Any Any...

...minipreps? If so, we’d love to have you write for us! Click Here to Contribute to the Addgene Blog Possible ...molecular biologist Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors Career development...

... Dives” series comes in! We’ll take a common application or concept and go beyond the basics - diving ...you’re ready for the next steps on some common applications, join us as we get into the weeds on some of...

...target and use FRET flurophores for a variety of applications. While editing this post, I learned a lot about...in their own research. All posts from July 2016 Click on the links below to read all of our excellent ...

...foundational research, training, startups, and real applications of SynBio. Chapter 3: Synthetic biology as the...synthetic genome project and constructing ever more complicated biological devices. Chapter 6: - Making research...

...will simply load an extra ladder or technical replicate (ie, leftover sample) to fill the relevant wells...using an antibody validated for your protein and application of interest or validating an antibody yourself...lysis Add sonication step to protein lysis Splice variants Check literature Posttranslational...

...where I foster dogs and help screen dog adoption applications. My most recent foster dog was with us for two...on the advisory board for Art in Giving, a 501c3 public charity raising funds for childhood cancer through...

... lend them many other functions for use in RNA splicing, imaging, and more. Abil says she is currently...says the new tool might even find therapeutic application in addition to their use in basic biology, for...

... (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the business” Attend...Academic Solos to Industrial Symphonies by Gwen Acton, Alicia Gómez-Yafal & Emily Walsh The Hard Truth About ...

... of 17 signaling pathways that are frequently implicated in cancer cell proliferation, survival, differentiation... make the library usable for pooled screens. Applications of the cancer pathways ORFs kit Wood and his...

...introduction to pCri, please see the original publication cited below. pCri plasmids are available either...comprehensive review of affinity tags and microbial applications." Biotechnology journal 7.5 (2012): 620-634....

... new method to explore the inner structures of delicate plants and other organisms without destroying ...competition. This competition has gained so much public interest for its stunning images that is has been...

...complete with all the fixings), as well as plenty of delicious desserts. We enjoyed a video highlighting the ...course of scientific research. What was once a complicated process of requesting and sharing plasmids has...

...neurons expressing nLightG or nLightR before/after application of NE (10 μM) and corresponding pixel-wise ΔF.... Sensitivity In ex vivo studies on mouse brain slices, nLightG and GRABNE were more sensitive than nLightR...