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We narrowed to 921 results for: Tro;

Showing: 511 - 540 of 921 results

  1. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...problems. For instance, it’s much more difficult to introduce a gene of interest into primary cells, so most...both purified CPP-tagged protein and an untagged control could enter cultured cells and activate expression...reporters Previous Protein Delivery Techniques: Introduction of macromolecules into cultured mammalian cells...

  2. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...reproduce someone else’s result. Then, they hit trouble. The experiment won’t work. Even if it does, they...to share with other researchers to help them troubleshoot and understand. All of this communication and... of incentives. Right now, researchers are not strongly rewarded for being transparent in their communication...

  3. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...transduction profiles in different AAV serotypes both in vitro and in vivo when compared to the conventional AAVs...target less accessible cells, such as brain tumor stromal cells in a mouse model of glioblastoma (Volak et...vector-mediated genetic modification of brain tumor stromal cells after intravenous delivery." Journal of neuro-oncology...

  4. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...independently verified, the watching world erupted in controversy. Briefly, there are two overlapping, valid critiques...within their respective mandates, to tackle a controversial and ethically fraught rapidly emerging technology...good research practice: computer simulations, in vitro research, animal models, and several stages of human...

  5. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...therefore higher titer will be required. For example, retrograde AAV gets transported up the cell’s axon. While...even larger area, thus necessitating even higher retrograde AAV titers for successful transduction. In another...from and what do they mean? AAV Vector Quality Control: Going the Extra Mile with NGS AAV:  Versatile ...

  6. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...components, it may be useful to describe the means of introducing genetic material (such as plasmids) into mammalian... comparable to bacterial transformation (the introduction of DNA into bacterial cells); however, the techniques...plasmid to be expressed transiently. Since the DNA introduced in the transfection process is not integrated...

  8. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...acid in the media and the tRNAs and synthetase introduced to the bacteria using plasmids. Rapid expansion...plasmid. Third, a second plasmid will be needed to introduce the gene of interest containing the chosen codon...researchers, from selective protein ligation through introducing ncAAs with protein modifications of interest ...

  9. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...which is more than just the stuff that makes bones strong; it plays critical roles in nerve and muscle function...fluorescent tools are used simultaneously to monitor or control different cell types or signals in the brain. For...Simultaneous fluorescence (upper trace) and electrophysiological recordings (lower trace) using OCaMP expressed...

  10. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ...growth rates will be higher in treatment versus control, and you think there are four important times to...preregistration, but usually with a fully fleshed out introduction section) to one of 222 participating journals..., they will place special emphasis on positive controls and good sample sizes, concerns that are too easy...

  11. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...promoters can be regulated by positive or negative control. Positive inducible In the OFF state, the promoter...system, the activator rtTA (reverse tetracycline-controlled transactivator) is normally inactive and cannot...

  12. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ... by stories. In retrospect, I should have expected this given that the brain controls everything we do... at Addgene.org Addgene's Viral Service Find Retrograde AAV Browse Optogenetics Plasmids ...

  13. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...this final installment, I’ll provide a brief introduction as to how you can engineer new transgenic flies...use attP sequence docking sites so that you can control exactly in which locus your transgene ends up (...selection markers include vermillion (another gene controlling eye color) and yellow (a gene affecting the body...

  14. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ... give you theirs! Think about how you want to introduce yourself and what you do. You can probably use... at a conference, but start with a more general intro until you know if the person you are talking to ...people in sessions by sitting next to someone and introducing yourself. Put your phone away! This is a natural...

  15. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    .... Unfortunately, LinkedIn has spam profiles and trolls. People don’t like to connect with complete strangers... much better to use your network to request introductions to people of interest for such discussions.  ...connection can help you gather information, make introductions, and give advice for your interviews.  Pro tip...

  16. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...,000 people globally. Pustules form from the uncontrolled activation of the interleukin-36 (IL-36) cytokine...macrophages, T cells, and the recruitment of neutrophils. Though there are IL-36 receptor inhibitors in... significantly reduced levels in women with TS (Trolle et al., 2016). Using lentiviral vectors from Addgene...

  17. Summer SciComm: Preprints

    Type
    Blog Post
    ...communicating the results of the work. Yes, those control experiments are important — but without letting...and finish that experiment (and don’t forget the controls!). And when you’re ready to write up the paper...blog Summer SciComm Series: Modes of Communication Intro to Our Summer SciComm Series!...

  18. Addgene: The First Twenty Years

    Type
    Blog Post
    ...institutions with standardized agreements and electronic signatures to dramatically speed up MTA approvals...Massachusetts Nonprofit Network Excellence Award for our electronic MTA system. We also started our blog and YouTube...biology and cloning, since they have reasonable introductions to lots of molecular biology,” — Reddit commentor...

  19. dTAG - You're it!

    Type
    Blog Post
    ... molecule degraders” recruit the proteasome to destroy targeted proteins. We previously developed small...lines Treat parental cells (or cells expressing a control -- for example, an FKBP12F36V-tagged LACZ) with...: dTAG-7 and dTAG-13 Erb et al. Transcription control by the ENL YEATS domain in acute leukemia. Nature...

  20. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...pitfalls of these experiments are discussed here. In vitro tests for aggregation The tendency of purified fluorescent...concentration 50% of the protein forms dimers. Finally, spectroscopic methods (based on fluorescence polarization)...respectively, are used as positive and negative controls for proper ER structure. Based on these data, ...

  21. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...attached to a substrate. These in vitro methods allow for a well-controlled environment, but they do not mimic...large number of emitted photons before they are destroyed. Factors that affect photostability The photobleaching...

  22. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...found that the study saved lives. Reduced use of estrogen plus progestin hormone therapy resulted in 76,...they worry about needing more study subjects and control groups. The important point, however, is that cost...Evoloving Check Out Extracurricular Activities for a Strong Science Career Path Additional Resources Check...

  23. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    ...find a title, author names and affiliations, introduction, methods, results, conclusions, and acknowledgements...flanked by two equally-sized columns, where the introduction, methods, conclusions, and acknowledgements ...the work. The other poster sections like the introduction, methods, and figures flank the central region...

  24. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...effective as it can result in the retention of introns or the removal of exons. Disrupting promoter or.... Find CRISPR plasmids at Addgene! Knock-in: Introducing tags or stop signals Once Cas9 generates double-strand...breaks, a new DNA sequence can be knocked-in, or introduced, into the break sites. Knock-in experiments are...

  25. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...including endothelial cell-pericyte, neuron-astrocyte, and cancer-stromal cell pairs.   Figure 2: Schematic...single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair pathways...

  27. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...MTA) approval  Send plasmids to Addgene  Quality control  In this post, we’ll explain how to use our deposit...expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression...recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain...

  28. 6 Tips for Grant Writing

    Type
    Blog Post
    ... to get past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor (your supervisor... typically includes a couple of sentences of introduction to my area, a line about the research I will...you can find some great information on ELISA troubleshooting and multiplex assays.   Additional Resources...

  29. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...sequence (Green et al, 2014). The second was an in vitro paper-based platform that allowed gene circuits ... an extensive infrastructure of molecular and electronic companion technologies that has transformed the...CRISPR/Cas9-mediated genotyping, as well as our electronic reader that provides portable quantification ...

  30. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ... recombinase.   Once the SSR of choice is introduced, recombination can proceed either by first utilizing...alone rescues the phenotype being studied. Introduction of a conditional point mutation or functional...transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic reporter would ensure...
Showing: 511 - 540 of 921 results