We narrowed to 643 results for: ato

1. Plasmids for Endogenous Gene Tagging in Human Cells

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   ...specific, individual proteins under endogenous regulatory control. But even with the advent of CRISPR/Cas9...

2. CRISPR in the Clinic

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   ... muscular dystrophy, cardiovascular disease, inflammatory diseases, blindness, and diabetes. Directions...

3. Viral Vectors 101: Chemogenetics

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   ...PSAMs PSAMs PSAMs, or pharmacologically selective actuator modules, were developed with the goal of creating...

4. Viral Vectors 101: Virus Safety

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   ..., some adenovirus strains are known to cause respiratory diseases in humans, earning this virus a BSL-...

5. Hot Plasmids and Viral Preps - January 2021

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   ...AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato is now available in AAV1 and AAV8, as well as AAV5...

6. 5 Tips for Troubleshooting Viral Transductions

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   ...sensitive to the toxicity of polybrene (e.g., hematopoietic or primary cells), and has been shown to increase...

7. Cpf1 Update: Comparison to Cas9 and NgAgo

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   ...barely fits into AAV when you add in a gRNA and regulatory elements. To solve this problem, the Zhang lab...

8. Gaining Leadership Skills Volunteering at a Professional Organization

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   ...the work I agreed upon (just as I would in my laboratory work). Committee members have to work professionally...

9. Experimenting in an Open Source lab: from CRISPR to Cats

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   ...trying to explore the links between genetics and anatomy, which are not yet fully understood. We attempt...

10. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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    ...graduate students since 2009. Dora is also the creator of the “Finish Your Thesis” online course.  To ...

11. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    ...for cancer gene therapy such as nonalcoholic steatohepatitis cancer. In deficiencies of plasma proteins ...

12. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    ...Murakoshi, H., Horiuchi, H., Kosugi, T., Onda, M., Sato, A., Koga, N., & Nabekura, J. (2019). ShadowR: a...

13. The Scientific Conference Poster Session: Tips for Success

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    ...minutes per poster. Ask the presenter to give the “elevator pitch,” of his/her project – kindly let the presenter...

14. Academic vs. Industry Postdocs

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    ...time working on something extremely risky or exploratory. Getting a job afterwards: Fairly or unfairly...

15. The AAVantages of AAV in CRISPR Screens

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    ...vivoscreening platform. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831 Santinha...

16. Production of Virus in Insect Versus Mammalian Cells

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    ...mammalian cells, they do not require serum or an incubator to grow. The virus Insect cell systems are used...

17. Magnetic Control of Proteins: More than a Dream

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    ...potential applications.  Andrew is a frequent collaborator and is happy to give advice and support for ...

18. Viral Vectors 101: Producing Your rAAV

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    ...Blouin-Tavel, V., Bosch, F., Bose, M., Byrne, B. J., Caton, T., Chiorini, J. A., Chtarto, A., Clark, K. R.,...

19. Plasmids 101: Protein Expression

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    ..., tobacco, rice, sugarcane, and even tubers of potatoes have been used for protein expression. Plant ...

20. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    ...editing Hu et al. also engineered transcriptional activators using the xCas9 scaffold. With an NGG PAM, xCas9...

21. Transferable Skills Guide: Managing a Team

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    ...delegate - as I quickly learned! I was a poor delegator at first. I knew what needed to be done and how...

22. Fluorescent Proteins 101: Fluorescent Protein Timers

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    ...Tsuboi et al.), limiting its use. Nonetheless, its creators noticed a few properties that have now become ...

23. Tips for a 1st time AAV user (by a Rookie AAV user)

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    ...tool for gene expression in mammals Use the PEI calculator for planning AAV packaging transfections Find...

24. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    ...catalytically dead dCas9 can be used in conjunction with activator or repressor domains to control gene expression...

25. Tips for Using BLAST to Verify Plasmids

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    ...such as full plasmid sequences provided by the laboratories that deposit their plasmids with us or other...

26. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    ...world using words like OR and AND. These logical operators are so useful they are implemented in most materials...

27. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ...technologies. Message or follow him on twitter @Vopinator.     References: Halpin-Healy TS, Klompe SE, Sternberg...

28. A Guide to Starting Your Own Journal Club

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    ...level of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal...

29. MXS Chaining

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    ...construction of plasmids with many components. The creators of the MXS-chaining method have used the technique...

30. Viral Vectors 101: The Retroviral Lifecycle

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    ... Cullen, B. R. (1992). Mechanism of action of regulatory proteins encoded by complex retroviruses. Microbiological...