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We narrowed to 1,029 results for: ELL

Showing: 681 - 720 of 1029 results

  1. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...degraded, cell still can methylate DNA   hsdS(rk-,mk-) Unmethylated DNA not degraded, cell cannot methylate... gal Mutation in galactose metabolism pathway Cells cannot grow on galactose only gyrA, gyrA96 DNA...permease inactivated, lactose cannot be taken up by cell mcrA, mcrBC Inactivation of pathway that cleaves...gene that maintains the plasmids at ~250 copies per cell.  F- ∆lac169 rpoS(Am) robA1 creC510 hsdR514 endA...rpsL20 xyl-5 leu mtl-1 Stbl4   Electrocompetent cells for cloning and storage of unstable DNA and lentiviral...

  2. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    ...institutions in Ghana and most recently got his PhD in cell and molecular biology at the University of Edinburgh...developing novel systems for bioconversion of cellulosic biomass to useful products under Professor Christopher...Plasmid Grant. For this grant, we’re working with fellow nonprofit, Seeding Labs, to distribute plasmids...Kwabena plans to use his research experiences as well as plasmids from Addgene to bring new opportunities...biology, DNA assembly methods, and engineering cellulose degradation. 18:21 - 23:26 : Kwabena's plans for...

  3. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...immunocytochemistry (ICC) when the sample is cultured cells or immunohistochemistry (IHC) with tissues), where...know if they co-localize at the same place in the cell? Or you know that the presence of several biomarkers...tissue sections, methanol- or paraformaldehyde-fixed cell samples, or other preparations. Sequential IF For...anti-CASPR (green), and anti-Kv2.1 (cyan). (B) Cerebellum labeled with anti-GABA-AR β1 (white) and anti-GABA-AR...hippocampus, or counting the number of puncta in each cell. When making figures for a paper or presentation...approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the National ...

  4. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...transferable skills while you are also learning to be an excellent scientist. Leadership and management Take a lead...always involved. Demonstrating your ability to work well with others can be done in many ways… and many labs...supportive editors so your first drafts are always excellent Technical knowledge and creativity Become a “...Employers and academic departments want to be able to tell that you know about something and are passionate...local networking and trade organizations Another excellent perspective on this topic can be found on the ...

  5. Let There Be LITE Plasmids

    Type
    Blog Post
    ... vectors and genetically target them to specific cell populations. They demonstrated use of the LITEs ...non-invasively, its use is not restricted to cultured cells but extends to freely moving animals, as established...novel mode of optogenetic control of endogenous cellular processes." The LITE system can now be applied...no means limited to particular model organisms or cell types. “This tool is really flexible and modular...supernatant. (The details can be found here.) So tell us, readers, how will you get creative with LITE...

  6. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...plasmids to upregulate their expression in specific cells. With a more concrete understanding of the molecular...molecules targeting SMN1 and its partners in mouse cell lines. Dr. Di Schiavi hopes that these studies will... Mesothelioma  Noushin Nabavi is a Postdoctoral Fellow at the Vancouver Prostate Center and is affiliated...that affects the lining of the lungs is relatively well known, there are a variety of lesser known mesothelioma... of ~30 patient derived peritoneal mesothelioma cells lines and assess their tumorigenesis. These experiments...

  7. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...families of cell surface and secreted proteins (glypicans, integrins, and more coming soon) as well as epitope...high-throughput screening techniques such as ELISA and cell surface display are common. ELISA enables quantitative...for comparing relative affinities across mutants. Cell surface display systems, such as bacterial, yeast...presentation of your mutant libraries on the surface of cells. This enables direct screening via flow cytometry...therapies and improve their delivery methods to various cells. The wealth of smaller engineered antibody fragments...

  8. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...statistical tests – when to use them, what they can tell you, and how to identify which one is right for ..., and mode give you a summary snapshot without telling you anything about outliers or data spread within...Distribution - spread of data; typically, normal (bell curve) or skewed (see Fig. 2). Assumptions – ... up to two independent variables. This test will tell you if there are significant differences between... the means of your groups. However, it will only tell you if differences exist somewhere within your data...crop production (though you would not be able to tell if the dependent variables worked synergistically...

  9. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...profit motive. We build this trust by keeping excellent records and maintaining confidentiality where ... Addgenies like the nonprofit company culture as well. We work every day to do a better job of enabling...Jessica, Joel, Jonathan, Justine, Karen, Kate, Kayla, Kelly, Kory, Kris, Leila, Lianna, Luke, Marcy, Mary, Max...Melina, Meron, Michael Lo., Michael Le., Michel, Michelle, Morgan, Natashia, Nicole, Olivia, Pamela, Racheal...Addgene Blog Learn about Our Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports Rare Disease...

  10. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...circuits are generally linked to dopamine (DA), a well-known reward chemical and neurotransmitter. But ...firing patterns (or the frequency of its release) are well understood, the direct effect of DA binding to a...the brain has been in vivo microdialysis of extracellular fluid (Darvesh et al., 2011). This technique... across the semipermeable membrane from the extracellular fluid into the perfused liquid. The probe then...detection of dopamine in flies, fish, and mice." Cell 174.2 (2018): 481-496.PubMed PMID: 30007419. PubMed...

  11. Hot Plasmids - October 2022

    Type
    Blog Post
    ...two-photon microscopy. Cell, 18, 3408 - 3425.e29. (2022). https://doi.org/10.1016/j.cell.2022.07.013.   ...voltages. This includes monitoring of fast bursts, as well as slow up/down voltage states in deep tissue, in...voltage fluctuations. Image credit: Liu Z, et al. Cell. 2022.  Find JEDI-2p viral preps here (and here...specific for human c-myc proto-oncogene product. Mol Cell Biol. Dec;5(12):3610-6.(1985). doi: 10.1128/mcb.5.12.3610...

  12. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...the fall colors are inspiring you to paint your cells in equally vivid colors, you'll want to check out...AAV9-X1.1 enables expression in brain endothelial cells via an injection into the systemic circulation. ...levels compared to AAV9. You can now target these cells with AAV-CAG-GFP packaged in AAV9-X1.1!  We also...recombinase or a fluorescent marker in the various cell types of the basal ganglia. They work via newly-...before. We have a bunch of other new AAV vectors as well — including next-generation biosensors for GABA,...

  13. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    ... remember what happened when drug X was added to cell line Y, or the phenotype of a mouse with a specific...comparison of the same experiment from different cell lines/model systems ReFigure allows you to make...the right experiment! The ReFigure extension also tells readers if a paper they’re reading has been linked... to show that a previously used reagent performs well, or you might publish a replication experiment that...immuno-oncology. Funding agencies such as the NIH and Wellcome Trust now accept interim research products (pre-registration...

  14. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...sweat, and tears, as well as the whole point of the paper. They should be able to tell your story for you...I am writing isn’t well written, I must write everyday in order to get better. Well what does that mean...interaction of critiquing others’ manuscripts as well as being critiqued added an extra level of application...

  15. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    ...to learn much more about cellular DNA repair pathways. An emphasis on cellular repair post-CRISPR cleavage... a common thread at the meeting. The new Somatic Cell Genome Editing program, run through the NIH Common..., which alter genomic DNA without the need for cellular HDR.   Having had time to digest my notes, since...Improve HDR Efficiency for CRISPR Editing in Human Cells Hands-on tips for repair template design from guest...

  16. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    ...these issues by creating immortal antibody-producing cell lines (hybridomas) that secrete antibodies in culture...reproducible production. However, even clonal hybridoma cell lines can produce more than one mAb (Bradbury et...about recombinant antibodies in this blog post. Hello, NABOR! Addgene will bring the same open-access ...resource of plasmids encoding recombinant antibodies as well as other affinity reagents such as single-chain ...need for an open-access, molecularly defined, and well validated collection. I’m thrilled to be working...

  17. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...R-factors could be transferred between bacteria via cell-to-cell contact; however, scientists noted that, unlike...cytoplasmic factors that could be transferred between cells. At the time, these extranuclear agents of heredity...PMCID: PMC3785787. References: Lederberg, Joshua. "Cell Genetics and Hereditary Symbiosis" Physiological...Transmissible Drug Resistance in an Epidemic Strain of Salmonella Typhimurium.” The Journal of Hygiene 60.3 (1962...

  18. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...to supplement your cells with biliverdin for maximal brightness. In mammalian cells, one of the improved...Imaging Center at UCSF. A common requirement for live cell imaging experiments is the ability to follow multiple...emission spectra of the protein, so that the protein is well excited by your microscope and the fluorescence ...laboratory of Ronald Vale, after which he was a fellow at the Bauer Center for Genomics Research at Harvard...

  19. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...recognized and valued for its immense importance, it may well be what determines whether you get the job after...as an opportunity to practice communicating.  Excellent scientists learn from advisers and other mentors...forget that you have a much bigger job than merely telling your audience about the work you've done. If you...help graduate students in all STEM fields become excellent science communicators. To empower graduate students...in science communication The strength of story telling Science communication: 9 strategies to get your...

  20. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...stimulate AraC binding to I1 and I2 sites. Supplementing cell growth media with glucose decreases cAMP and represses...Steroid-regulated LexA promoter XVE (synthetic) pFZ19 (yellow flame) Temperature inducible promoters Temperature... Because transcription machinery differs between cell types or organisms, promoters are similarly variable...variable. Bacterial promoters only work in prokaryotic cells and typically only in the same or closely related... were derived. Similarly, the various eukaryotic cell types (mammalian, yeast, plants, etc.) require unique...

  21. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...team potential. I often thought that performing a cell culture experiment myself would take less time than...Another team member was highly self-motivated and excelled when given the freedom to explore projects autonomously...management skills. Finally, developing emotional intelligence is vital. Learning to "read the room," understanding...part of your broader career development.   Matteo Tardelli authored the book "Beyond Academia: Stories and...molecular biology and metabolism. He now guides fellow PhDs in their careers with actionable steps and...

  22. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ... Many thanks to our guest blogger David Mellor! David Mellor leads the policy and incentive programs ...This post was contributed by David Mellor from the Center for Open Science. In the last decade, researchers...Before coming to the Center for Open Science, Dr. Mellor worked with citizen scientists to design and implement...at https://orcid.org/0000-0002-3125-5888 and @EvoMellor.  ...

  23. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...can also find Dr. Page and Hello PhD on Twitter: @ThePurplePage and @HelloPhD "Role" call. #BLACKandSTEM...Science Communication” at the American Society for Cell Biology (ASCB) 2018 Conference. I don’t exactly ...Scientists SHOULD Be Using Twitter" podcast from Hello PhD In this podcast the hosts, Josh and Dan, discuss...there’s some great reminders for Twitter pros as well, like tip #4: “Follow people for about a week and...

  24. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ...have lunch or coffee with someone I don’t know very well at least twice a week. I have done this with people... career advice. Your family and friends know you well and might have good ideas for who you should connect...Healthcare Businesswomen’s Association hosts an excellent group mentoring program in many of its chapters...keep working with you is report back with progress. Tell them how their advice made a difference, helped ...in a leap in your skills. If a first meeting goes well, you can say, “This was really helpful, perhaps ...

  25. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    ...thinking. Being able to learn things quickly. Story telling. The ability to know what’s a story and how to ...to hook your readers with it.” She feels that storytelling is an impactful tool for science communicators...through connections and that’s something that good storytellers in science can do.” Susan’s number one piece... starting a blog, but with one caveat: “I hate telling people to work for free because you shouldn’t work...social media as a scientist here Learn more about storytelling in science ...

  26. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...system, antibodies are produced by B cells. Each individual B cell produces antibodies that all recognize...also all the same isotype. But together, all the B cell clones in the immune system make different isotypes...antibodies starts with selecting just one of these B cell clones for further antibody production, polyclonal... blood from the immunized animal. The red blood cells and the serum are separated, and the serum - which...

  27. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...Professional Career Coaching can be an excellent tactic for scientists making a career shift or who wish...of this to get the information you need to choose well. What are their qualifications? Do they have certifications...does not endorse any of these coaches and I can't tell you which one will be a match for your needs, but...Consulting is part of that group and she is especially excellent in coaching for communication skills. Industry...Industry transition is also an area of specialty for Michelle Capes at Adeptify Me. While based in Madison, ...

  28. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ... question about what should go into a cell, hover over the cell and instructions will pop up. When you...spreadsheet (Figure 2) and are described in Table 1. Some cells have drop-down options for you to choose from, while... into the appropriate full and partial sequence cells. *Note: If you believe you need to deposit a collection...Relevant Mutations Indicate the amino acid change as well as any functional consequences of that change. For...

  29. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...currently doing this using two approaches: single-cell RNA-sequencing to identify neuronal class and deep...Drosophila and zebrafish articles published there as well.   Worm Development Dynamics Database (WDDD) The...WDDD aims to acquire quantitative data on all the cell divisions of the C. elegans embryo when all essential...Wang et al., 2018).” With the split system, only cells that have both halves of cGAL can drive expression... Alyssa Cecchetelli...

  30. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...better than a nitrocellulose membrane. If you have already run your blot using nitrocellulose, you may decide...protein of interest in a complicated solution like cell lysate. But they can be a lot of work — and what...instructions for the membrane you are using. Dry nitrocellulose membranes are brittle, so proceed with caution...to solutions. It is also volatile and toxic (and smells bad!), so be sure to use it under a fume hood. ...

  31. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...encode proteins. They play important roles in many cellular and regulatory processes, but much of their functionality...RNA (sgRNA) to create a double-strand break works well because very small deletions and insertions in the... should be able to devise an experiment that can tell you more about its function.  Resources and references...screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Nat Biotechnol...non-coding RNAs with the CRISPR/Cas9 system in human cell lines. Nucleic Acids Research. 2014;43(3):e17. doi...

  32. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...plethora of antibody options for your experiment (all well validated for your application, of course!) When...the isotype, and subclass affect my experiment?” Well, it depends on the application and target. If you... blot and probing a single target that expresses well, then probably not. In this case, take a look at...degree of affinity, then species choice could very well matter. Take rabbit versus mouse monoclonal antibodies...for Light and Electron Microscopy. In Methods in Cell Biology (pp. 283–310). Elsevier. https://doi.org...

  33. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...since the lab was highly focused on protecting intellectual property, it worked away in isolation. It published...joint. Silly ideas (mining local salt deposits to sell at farmers markets), and serious ideas (how to stop...color-changing flowers was a great business idea as well, and they thought we could be taught. They invested...Biochemistry Department at the University of Manchester as well as the Biology Department at CSU. When not pursuing...enjoys spending time with her husband and two Jack Russell Terriers. More SynBio Reading Read more Addgene...

  34. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...overexpress YFG in a Drosophila cell line. Knock downs in Drosophila cell lines are extremely effective....days after setting up the genetic cross). You can tell when pupae are ready to hatch the next day because...person. Animals are diploid except for their germ cells (sets of genomic material are contributed by mother...various fly reagents on the Bloomington website as well. Additionally, they have useful information on topics...

  35. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ... in mammalian cells Hu et al. compared xCas9 activity with wt SpCas9 in mammalian cells, finding xCas9...ABE7.10, as well as improvements at NG and GAT PAMs. xCas9 3.7 Activity in Mammalian Cells Construct... percent editing efficiencies (edit frequency in cell population measured with NGS) are shown as percentages...

  36. Academic vs. Industry Postdocs

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    Blog Post
    ...doing a brief academic postdoc. Why did I switch? Well, my long-term goal was to enter industry since I...” that you’ve sold out. You’ve given up your intellectual freedom and integrity. (Not the case, as I’ve...s ability to manage others and work as a team as well as their scientific abilities. At AstraZeneca, scientists...who get postdocs and do not support or manage them well are simply no longer allowed to have postdocs. In...Scientist at AstraZeneca. She has a background in cell and developmental biology and is applying this towards...

  37. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ... for Mapping Multiprotein Complexes in Human Cells.” Cell Rep. 13: 621-633. PMID: 26456817. Find plasmids...Chromosomes). Using their PITCh plasmids, GFP knock-in cell lines can be created in about a month and a half...locus-specific gRNAs Step 4: Select for puromycin resistant cells Step 5: PCR amplify and sequence the locus to verify...crispr.mit.edu. They tested their system in HEK293 cells, integrating the GFP-Puro cassette into the FBL ...

  38. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...encoded tools, such as microbial opsins, to control cellular activities using light. In 2015, scientists combined...activator domain (VP64 or p65). Upon expression in the cell, the dCas9-CIB1 fusion binds to the target DNA sequence...activator domain VP64. Using this system in HEK293T cells to induce expression of human IL1RN, the researchers...protein with three red fluorescent tags in U2OS cells. Upon light activation, dCas9 is released within... silencing of transferrin receptor CD71 in HeLa cells.   Comparison of photoactivatable CRISPR strategies...Spatiotemporal Control of CRISPR/Cas9 Function in Cells and Zebrafish using Light‐Activated Guide RNA. Angew...

  39. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...Nanobodies are like tiny antibodies which work just as well, if not better, than antibodies for all of the above...structure and function compare to IgG antibodies, as well as how to produce them for use in your lab. Structural...triple colocalization is possible even when staining cells with three IgG1 antibodies. Antibodies were individually...workflow yields similar localization patterns as cells stained for one target (compare nanobodies staining...–rabbit IgG secondary nanobodies. The Journal of Cell Biology. PubMed PMID: 29263082 Find plasmids from...

  40. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...machinery, and could thus be used in non-dividing cells. Cas13 enzymes also don’t require a PAM sequence... RNA knockdown. In initial tests, Cas13b from Prevotella sep. P5-125 (PspCas13b) yielded 62.9% average... a maximum of 28% editing efficiency in HEK293T cells, as assessed using RNA-seq. Since the REPAIRv1 machinery...including reversibility and use in non-dividing cells. With the expanded targeting ability using both ...Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes." Mol Cell. 66(3) (2017):373-83. PubMed PMID: 28475872. Find...
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