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We narrowed to 1,014 results for: REN;

Showing: 851 - 900 of 1014 results

  1. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...provides something that you can’t obtain at your current institute. A common reason is hands-on training... right for you, start thinking outside of your current research box, send out some feelers, apply to programs...

  3. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...things you can do to prepare. Adapt to WFH while parenting Keep your kids on a schedule so you can rely ...Most Micropublications contain one figure. If you aren’t there yet, write introductions to potential publications...

  5. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...multiple samples without cross contamination. We currently use the CosMCPrep system for this, but thought... because it forced workshop participants from different biotech companies to interact and learn more about...

  6. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...have made it easier to track translation in two different ways: by monitoring the first round of translation...various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide ...

  7. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...permeable to CO2 at low temperatures. We tested two different types of tubes: the ones we use to store lentivirus...also using the thawed virus within 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer...

  9. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...the interview process was several short talks by current faculty members and it just blew me away….  I have...overall mission of making science easier and more transparent. That’s largely my job [at the Broad] too… to...

  10. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...interests, and spends one-on-one time with the current members to share short-term goals and chat about..., but even brief stays there can make all the difference. Choose a lab that brings on the happy dance ...

  11. Hot Plasmids - May 2022

    Type
    Blog Post
    ...peptide. By combining three different localization peptides with four different fluorescent proteins, and...

  12. Antibodies 101: ChIP

    Type
    Blog Post
    ...optimized for each specific sample type. And, if that weren’t bad enough, optimization of one step can affect...protocols when embarking on your ChIP journey!  References and Resources More resources on Addgene’s blog...

  13. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...projecting to the inferior colliculus (IC) are differentially transduced when using the retrograde serotype...significantly updated in 2023 by Jason Nasse.  References 1. Tervo, D. Gowanlock R., et al. "A designer...

  14. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...deliver cargo, it’s important to understand their differences to choose the best one for your experiment. ...Here to Find Viral Vector Resources at Addgene! References and Resources 1. Roe T, et al. “Integration of...

  15. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...illustrates the utility these tools have in many different areas of biology as they grow. The top 15 plasmids...envelope protein enables this virus to infect many different cell types so it is useful in a wide range of ...

  16. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...and mEGFP. Many of these features, such as Cas9, weren’t detected by our in-house software. In fact, SnapGene...static image file (PNG, see above) for your own reference or to paste directly into your notebook. This ...

  17. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...contain 3 methylases that recognize and methylate different stretches of DNA: Dam methylase adds a methyl...could overlap with a methylation site. The quick-reference table below lists 10 common enzymes that may be...

  18. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...your working directory (the folder that you are currently working in) every time you use them, leave them...guest blogger Kate Palozola! Kate Palozola is currently a graduate student at University of Pennsylvania...

  19. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...and Gammaproteobacteria species isolated from different bee species. BTK can be used to introduce fluorescent...distributed cell division counting plasmids here References Leonard, Sean P., et al. "Genetic engineering...

  21. Addgene: The First Twenty Years

    Type
    Blog Post
    ...organizations, knocking on doors, and attending conferences. And when CRISPR arrived in 2012, our repository...’s booth at the Society for Neuroscience 2023 conference. In 2020, the world changed in response to the...

  22. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...whether or not the tags influence behavior. A conference and a breakthrough! During my graduate studies...you can contact her at [email protected]. References 1. Dieterich, Daniela C., et al. "In situ visualization...

  23. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...probably aware that there are several methodologies currently available for approaching it. These include the...even to submit a guest blog entry of your own! References 1. Bernard, P. “Positive Selection of Recombinant...

  24. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...fail you. The benefits of lab management software aren’t just limited to large volume facilities; it can...above, any one of these steps can fail but, in our current setup, there are no explicit instructions on what...

  26. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...same type, but are not cross-compatible. Using different lox site variants allows Cre to catalyze more ...cre-dependent techniques?  Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination...

  30. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...dividing up the essential components of a virus into different plasmids. For a retroviral system this looks like... health and safety department if they are not currently provided. Eye protection or face shields are ...

  32. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ... discrimination is especially important in the current outbreak because of the neurological symptoms associated...applications in health, energy, and sustainability.   References 1. Pardee, Keith, et al. "Rapid, Low-Cost Detection...

  34. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...immunoprecipitate the entire complex in three different cell lines. The Gräslund lab plans to continue...of indels to measure time. These plasmids with different promoters driving expression of Cas9 were used...

  35. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...will produce the same unique SapI 5’ overhang, different combinations of connector and tag donor plasmids...light on previously unknown protein functions. References Schwartz, Matthew L. and Eric M. Jorgensen. “...

  36. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ... new eBook! Our goal was to create a one-stop reference guide for plasmids. We've combined our Plasmids...solutions necessary for advancing the field. References: 1. Internal initiation of translation of eukaryotic...

  37. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ... acidic, or synaptic vesicles). Some FPs have different ex/em spectra (e.g. mKeima) or change fluorescent...Fluorescent Protein (GFP) Three Review Articles on the Different Types of FPs: Stepanenko et. al. (2008) “Fluorescent...

  40. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...enjoy and that plays at least slightly to your strengths. Otherwise, you’re going to drop your project ...scientific training. Cartoons may be read as “for children” or may need text for audience interpretation....

  43. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...editing efficiencies in a number of cell types at different genomic loci — in some cases, over 20-fold higher...prime editor. B, Prime editing efficiencies at different loci in U2OS cells. Image adapted from Yan et ...

  44. Viral Production at Addgene

    Type
    Blog Post
    ... by Ina Ersing in August 2023. Resources and references Resources at the Addgene blog Find and share ...Quantification Viral Vectors 101: An Introduction to AAV References Lock, M., Alvira, M. R., Chen, S.-J., & Wilson...

  45. How to Be an Excellent Trainee

    Type
    Blog Post
    ...protocol you don’t understand the purpose of, or a different way of doing something then you’ve done previously...an active partner Everyone’s learning style is different, so let your mentor know what yours is! For instance...

  47. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...FRET experiments. You may have to design several different constructs to find one that could be used for ...Fluorescent protein FRET: the good, the bad and the ugly. Trends Biochem Sci. 2007 Sep; 32(9): 407-14. Zimmermann...

  49. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...tasks. If you’re constantly switching between different projects, you might not be able to make any reasonable...components: A mechanism for blocking off time for different tasks. I use google calendar for this and try ...

  50. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...Over 25 million people worldwide are currently infected with the lentivirus HIV-1. Today, HIV-1 can be...for some of the world’s most common diseases. References Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski...
Showing: 851 - 900 of 1014 results