...Balancing research with medical practice as an MD/PhD. Darrel does 20% clinical work 80% research and was...for Regenerative Medicine at Boston University Medical School. Darrell’s research focuses on the lungs...Center for Regenerative Medicine, and within the biomedical research community. Along these lines, Darrell...Pathology and Laboratory Medicine. He attends in the Medical Intensive Care Unit, the Pulmonary Consultation...the Pulmonary outpatient clinic, all at Boston Medical Center. In addition he conducts basic stem cell...Regenerative Medicine of Boston University and Boston Medical Center. Special thanks to our sound engineer/producer...

...often scarce and difficult to identify. So what specifically should you do? What follows are just a few suggestions... in fact, some of the very things that were so critical to me landing my own position! Conferences One...Society for Cell Biology (ASCB) or the American Chemical Society (ACS) are just 2 examples of organizations...will sometimes result in a recruiter giving you a call. Many companies enlist the assistance of recruiters...appropriate job candidates. If you do receive such a call, make sure to get that person’s contact information... get the specific position he or she originally called about, you can keep in touch and reach back out...know all in one place! This information can be critical to a focused and effective job search. As with...

...have been used since the 1930’s in a range of biological studies, including investigations into environmental... are powerful resources for investigating any biological process. High quality genome sequence A high-... mutants In the 1990’s, two revolutionary large-scale random mutagenesis screens produced ~1,500 zebrafish...thousands of mutations in genes affecting diverse biological processes—not just development—are available ... zebrafish are short, modified oligonucleotides called morpholinos. When injected into zygotes, morpholinos...One common and robust strategy uses a transposon called Tol2 to randomly integrate foreign DNA into the...Naturally, the best model organism depends on the biological question, and zebrafish are not ideal for every...

... Predoctoral Fellow at University of Rochester Medical Center. During development, complex genetic programs...central nervous system (CNS). To address this technological gap, a trio of recent papers describe the development...Cre under neuron-specific promoters, including calmodulin-dependent kinase II alpha (aCaMKII; excitatory...with different untranslated or coding regions. Typically, each gene isoform has a distinct transcriptional...demonstrated by the Qi Lab, which developed a subgenome-scale CRISPRa screening approach in embryonic stem (ES...Miller-Rhodes from the University of Rochester Medical Center. Patrick Miller-Rhodes is currently a Ruth... Predoctoral Fellow at University of Rochester Medical Center. He studies gene-function relationships ...

...Within that research group, you will also typically receive technical mentorship from a ‘bench mentor’. In ...receive after you’ve found your mentor, though. Typically, you will identify a ‘scientific mentor’: someone...scientific project. Your scientific mentor is typically a professor who operates a research group. Within...component. Documenting during training It’s hard to recall a lot of details, especially when you are in a ...to do? Walk through the protocol mentally (or physically where relevant) and identify any gaps or questions...bring them to your mentor. Pro tip! Consider physically walking through the protocol, by going to each...experiments. Unlike in lab courses, where everything is magically made up by helpful TAs or assistants, and sitting...

... the Biotechnology and Biological Sciences Research Council (BBSRC) and Medical Research Council (MRC)...have tended to avoid cell culture in favour of biochemical assays followed by in vivo testing. The mantra...Estimates of the amount of money wasted in basic oncological research due to contamination are in the tens...prevented and we can progress faster to desired clinical endpoints.   Nick Amiss is an ATCC Outreach Scientist...experience through working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune...talks and seminars on cell culture and other pharmaceutical topics. The LGC Group also provides standards...Learn about the Dangers of Using GFP for Protein Localization Resources on Addgene.org Find Plasmids for...

...for neuronal/CNS expression. Modulated by calcium and calmodulin. GAL1, 10 General expression mRNA Yeast... promoters only work in prokaryotic cells and typically only in the same or closely related species from... be ways to turn them on or off at will with a chemical, heat, or light. In the cell, promoters themselves...scientists have created synthetic promoters, which typically include some combination of other promoter elements...Inducible with Tetracyline or its derivatives.  Typically contains a minimal promoter with low basal activity...CaMKIIa Gene expression for optogenetics mRNA  Ca2+/calmodulin-dependent protein kinase II promoter Specific...phosphage dehydrogenase Constitutive  Very strong, also called TDH3 or GAPDH. ADH1 General expression mRNA Yeast...

... request for phages to the directory does not typically come out until nearly the last minute when all...all other options have been exhausted. Theoretically, phage alerts can be sent out to the directory earlier...team must decide which labs should receive the clinical sample to test their phages. Because the sample...phages targeting organisms closely related to the clinical sample. For treatment, it is best to identify ... only used phage in basic research but not therapeutically: not all labs in Phage Directory have the resources...resources or know what it takes to prepare phages for clinical use. There are also different regulations from... individual phages. Integrating lab protocols, clinical information, phage research job postings, and ...

...Chinese Academy of Medical Sciences University of Texas at Austin University of California, San Francisco  ...scientific community support open sharing. But, one critical part was missing when the COVID-19 plasmids first...Children's Hospital                           California Institute of Technology             Johns Hopkins...                           National Institute for Physiological Sciences Broad Institute                                       ...part of research projects, another way is to systematically offer thousands of constructs for tagged and...enables research on solute carriers (SLCs) for medical research Recently, the Michael Davidson Fluorescent...

...also more precise. Base editors, for example, typically edit all the target bases within the base editing... target nucleotides are positioned within the canonical base editing window, base editing has higher efficiency...products to be indels while the efficiency of HDR is typically less than 10%. In contrast, the original paper...maximum efficiency isn’t everything — prime editing typically needs to be optimized for each application, and...nickase fused to a pentamutant of M-MLV RT. They called this system PE2, which had prime editing efficiencies...optimized for human codons; additional nuclear localization sequences; and two mutations in Cas9 previously... 3′ end, the Liu lab appended RNA pseudoknots, calling this longer RNA an “engineered pegRNA” or epegRNA...

...GFP is tagged with a nuclear localization sequence, allowing it to localize to a different area of the ...mOrange2, EGFP, and mKate2 (red) to solve the problematically low fluorescent intensity of previous versions.... XFPs are introduced using a plasmid construct called Zebrabow-GateDest containing an RFP, CFP, and YFP...developed a version of Tetbow that incorporates chemical tags instead of XFP to optimize it for tissue ...architecture of a fluorescent neuron is visible. Chemical tags are more resistant to tissue clearing methods...neuronal circuits with fluorescent proteins and chemical tags." eLife 7 (2018): e40350. PubMed PMID: 30454553...homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells." Cell 143.1 (2010)...

...short-lived biotin-phenoxyl radical in the presence of hydrogen peroxide. This radical reacts with electron-...capture these interactions in a vast array of biological contexts.  Proximity labelling relies on the ...and determine the topology of the mitochondrial calcium uniporter (Martell et al., 2012). It is important...RNA-protein interactions that can regulate multiple biological functions. RapID (RNA-protein interaction detection...Cell–Cell Interactions." Journal of the American Chemical Society 141.5 (2019): 1833-1837. PubMed PMID: ...et al. "Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy." Nature...total number of protein molecules per cell volume? A call to rethink some published values." Bioessays 35.12...

... important proof of concept for future preclinical and clinical work. Find Plasmids for Disease Research...research, researchers are eager to apply it in a clinical setting. CRISPR is often used for animal germline...exon skipping is a good option for DMD treatment. Clinical trials have used oligonucleotide exon skipping...rate of exon 51, which resulted in appropriately localized dystrophin. In a subsequent study, they used CRISPR...six months. Future directions and obstacles to clinical CRISPR editing Given the success of the CRISPR-mediated...be evaluated separately, increasing the time to clinical approval. Even with the challenges precision editing...become one of the first diseases to be treated clinically with CRISPR. Many obstacles remain for CRISPR...

...generally linked to dopamine (DA), a well-known reward chemical and neurotransmitter. But aside from DA’s famous...specific dopaminergic neurons in a brain region called the substantia nigra, which is the main source ... it can be performed in a live awake animal by surgical implantation of the probe into a specific brain... animals (Patriarchi et al., 2018). Similar to calcium and glutamate sensors, the new dLight1 sensor is...in freely moving mice Record DA dynamics of optogenetically activated and inhibited neurons in live mice...neuronal imaging of dopamine dynamics with designed genetically encoded sensors." Science 360.6396 (2018): eaat4422... PMCID: PMC6287765. Sun, Fangmiao, et al. "A genetically encoded fluorescent sensor enables rapid and ...

...swimming where they periodically beat their tails left and right in a symmetrical motion to move forward...behavioral studies. In neuroscience, opsins are typically ion channels that allow charged particles to cross.... The non-specific cation channel ChR2 and the calcium channel LiGluR both cause ion movement that results...Kolmer-Agduhr cells was sufficient to trigger the symmetrical tail movement unique to spontaneous slow forward... photostimulation in order to drive the full symmetrical response.  The function of Kolmer-Agduhr cells...Aristides B., Filippo Del Bene, and Herwig Baier. "Optical control of zebrafish behavior with halorhodopsin...

...to mind when we think “biomedical research". The family entered the biomedical field’s spotlight after...ways these animals have impacted humans through biomedical research. The Camelid family is composed of ...chromobodies,” which are single-domain antibodies genetically fused to fluorescent proteins and used to visualize...therapeutic was approved in 2018, with many others in clinical trials (Jin, et al. 2023). In addition to therapeutics...) (Xu, et al., 2021).      Figure 2: Genetically modified mice offer an alternative method to ...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...

...strengthened my critical thinking and problem solving abilities, increased my technical know-how, and helped...scientist.” For jobs away from the bench, look for “technical or product support specialist,” “field application...application scientist,” or “scientific or technical writer,” among others. Don’t discount job postings looking... you’re interested in (i.e. biotechnology, pharmaceuticals, etc.) Do their jobs sound interesting? Is ...deeper foundation of scientific knowledge and technical know-how that increased my autonomy and satisfaction...from the lab to a desk, although there’s often technical career tracks for PhDs who want to stay at the...

...Bryan Roth’s lab created a collection of plasmids called TRUPATH to interrogate 14 of the 16 non-visual ...TRUPATH collection allows researchers to measure the physical association of combinations of different Gα, Gβ...acceptor. TRUPATH uses an updated version of BRET called BRET2. Figure 1: TRUPATH measures heterotrimeric...N-terminus of the protein. For the Gα constructs, localization of the Rluc8 was more challenging. The Roth ...experimental refinement to determine the optimal localization of the Rluc8 insertion within the Gα. Despite...transducers and that the potency and efficacy is typically non-uniform across the transducerome for a specific...well known and, as a result, a target of many pharmaceutical drugs, there is still only a partial understanding...

...pAAV-hSyn-DIO-EGFP   Biosensor AAV Calcium sensors Tracking calcium dynamics that govern such activities... from Christopher Harvey’s lab. These plasmids localize opsins to the soma to avoid off target photostimulation...need to determine these transduction patterns empirically for their own experiments. To this end, we offer...studying neural networks. We have expanded our calcium sensor collection to include our first Flp-dependent...Several new options are also available for the calcium sensor jGCaMP7 from Janelia, including three jGCaMP7...technology, see our blog post Overview of AAV Encoded Calcium Sensors). Plasmid Serotype Name 104489 AAVrg...

...bacteria) may be the most numerous and most diverse biological entities on our planet, but we still know collectively... refractory to enzymatic digestion, however, a physical fragmentation strategy is preferable. Nebulization...tank with a pressure regulator. Other methods for physical shearing exist as well, such as sonication and...transform your resultant plasmid libraries into chemically competent E. coli and analyze transformants for...reduce the number of cells that will bind non-specifically to either the membrane itself or to any “sticky... antibody or other protein on hand that will specifically bind your target organism, it can be helpful...2015): 185-191. PubMed PMID: 25708933. 2. Cooper, Callum J., Mohammadali Khan Mirzaei, and Anders S. Nilsson...