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We narrowed to 993 results for: Des

Showing: 341 - 360 of 993 results

  1. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use in ...CRISPR plasmids that have been designed for use in C. elegans. CRISPR... can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...

  2. CRISPR in the Clinic

    Type
    Blog Post
    ...introducing a desired edit, but this particular class of treatments has quite the opposite aim: to destroy. One...potential for treatment is no longer an accurate descriptor; progress in clinical therapy is more fitting...One treatment currently in clinical trials includes Cas3 (the shredder member of the Cas family) as part...CRISPR therapies have made significant clinical strides since we first wrote this blog post. The future...

  3. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...of the Retroviridae or Retrovirus family, which includes the gamma-retrovirus and lentivirus genera, have...dividing and non-dividing cells. More detailed descriptions of the lentiviral elements and the 2nd and 3rd...#48201) This retroviral vector from the Gage lab encodes GFP and Cre-recombinase. The Cre-lox system is ...building block Entry vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial...single ecotropic or amphotropic MuLE lentivirus provides significant genetic power and allows high throughput...

  4. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...widely used in neuroscience. In this post, I’ll describe how rabies virus (RABV) can be used in the brain... by modifying the wild-type RABV genome, which encodes a single envelope protein called rabies glycoprotein... neurons integrated into the brain over time (Deshpande et al., 2013). The study found that at early time...Dec; 18(6): 617–623. Pubmed PMID 19349161.  2. Deshpande A, Bergami M, Ghanem A, Conzelmann KK, Lepier ...Pubmed PMID 26190977. 6. Osakada F, Callaway EM. Design and generation of recombinant rabies virus vectors...

  5. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...Plasmids designed to express genes in a given host cell type are generally broken down into two broad...post-transcriptional additional of multiple adenine (A) nucleotides to the tail of a messenger RNA transcript. The...structure of the hairpin-DNA complex is thought to destabilize the transcription complex, initiating cleavage...transcription of the template and initiating the desired cleavage event, although some engineered terminators...downstream elements. A high affinity terminator may be desired for multi-cistronic constructs where high termination...

  6. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ... asking the panelists not to use any slides for the event. Slides are for presentations, not discussions...discussions. I will rarely work with a panel if slides are allowed. Don't put your panelists behind a table. ...organizations, I asked audience members to stand and describe unique nonprofit models that differed from the...around to meet and greet audience members. Having dessert, drinks and/or coffee available only after the ...

  7. Bacterial Expression Systems

    Type
    Collection
    ... cleavage sites. Other protein tags and signal peptides are used to enhance protein solubility and to ...Commonly used tags, cleavage sites, and signal peptides include: Epitope tags: 6xHis, Flag, Strep II, ...protease, factor Xa, enterokinase, thrombin Signal peptides for periplasmic localization: PelB, MalE, OmpA...Anhydrotetracycline (aTc) Escherichia coli Stanley Qi 11518 pDest-527 T7-lacO Lactose/IPTG Escherichia coli Dominic...a collection of CRISPR plasmids that have been designed for their use in bacteria in our Bacterial CRISPR...

  8. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...138152 pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro Lentiviral vector expressing destabilized EGFP...CoV-2), modification, or variant. ID Plasmid Description Mutations Industry PI Due to export control regulations...neutralizing activity are listed below: ID Plasmid Description PI 44965 NL4-3 mCherry Luciferase Lentiviral ...

  9. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...addition to Taq DNA polymerase, PCR requires free nucleotides (dNTPs), template DNA to amplify from and unique...polymerases require an existing strand of DNA to add nucleotides to.  Using these reagents and a series of heating...protocol page, protocol video and reference on how to design PCR primers.  Types of PCR Since the invention ...  Digital droplet PCR or ddPCR is a method that provides ultrasensitive and absolute nucleotide concentration...Polymerase Chain Reaction (PCR) Protocol: How to Design a primer  Molecular Biology Reference Molecular...

  10. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...library, each plasmid encodes the mutated spike protein and a barcode, and is designed so that the SARS-CoV....1074/jbc.RA120.01289     Novel protein cage provides simple enzyme protection in yeast bioproduction...enzymes in a reaction pathway, the MPyV system provides a simple, efficient method for protecting a single...- Ampl133 targets S. cerevisiae genes with 6-12 guides per gene where gRNA expression is induced by anhydrous...

  11. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...fluorescent proteins, it’s important to note that despite their distinction by name, chromoproteins are actually...chromoproteins have a shorter history. In 1987, scientists described an unusual blue-colored protein from the jellyfish... chromoprotein plasmid that combines the most desirable features: intense color, fast maturation, and ...recombination events: The plasmid carrying the desired genetic modification integrates into the genome...rhamnose-inducible promoter. The resolution step leads to the desired mutation and excision of the vector backbone. To...

  12. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...., 2025). By co-delivering TRV1 with TRV2 that encodes ISYmu1 and an ωRNA under the same promoter, they...different applications require careful selection or design to ensure reliable readout and avoid biases. The...ex vivo HSC expansion cultures. A) Experimental design. B) UMAP of cell states in ex vivo expansion cultures...only at excitatory synapses; however, its modular design allows components to be swapped out, potentially... Huang, H., et al. (2025). ATLAS: a rationally designed anterograde transsynaptic tracer. Nature Methods...

  13. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...Cas11. Type I-E Cascades contains all five of these Cas proteins, while type I-C Cascades do not contain... 1: Schematic of Class 1 CRISPR types. Class 1 includes type I with a Cas3 effector, type III with a Cas10...CRISPR transposase systems utilize with type I-F Cascades. Figure 2: Cascade complex from CRISPR ...maximum efficiency of the process. Various labs have designed all-in-one Cascade plasmids — such as the Tomoji...

  14. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...fluorescent output. The GEVI family of gadgets includes Ace, JEDI, and many more. They can be targeted...component as well as a small molecule which we will describe in more detail below. Types of Voltage Indicators...Drosophila to phosphatases from sea squirts. This design structurally separates the two components of the...membrane, a concern with several of the previously described systems. Several adaptations of this method have...voltage sensing component (i.e., rhodopsin naturally resides in the membrane), or both. Targeting these systems...

  15. Pooled Library Amplifications

    Type
    Blog Post
    ...(Are the inserts of different sizes?), and the desired coverage for the library (How important is it that...  The most important factor to consider is your desired library coverage, since this will help decide what...similar in size and composition as examples. Table 1 provides an overview of pooled library protocols you can...should always experimentally test the protocol you design to ensure that it works for your library before...Addgene. If you have any specific questions about designing your library amplification protocol, feel free...

  16. Of Myc and Men

    Type
    Blog Post
    ...append their proteins of interest with unrelated peptides that already had high quality antibodies available...existence of high quality antibodies for well defined peptides. Munro and Pelham’s first published peptide tagging... tumorigenesis.  Using two different synthetic peptides corresponding to linear sequences within the middle...that perfect. One of the known limitations was described by Schüchner, et al., who published a paper showing...works and allow users to better troubleshoot and design experiments. It turns out that even with the most...

  17. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...such a system is likely to introduce additional nucleotides flanking the insertion. If the DNA ends anneal...MMEJ may also introduce, substitute, or delete nucleotides in addition to the expected deletion, but the...Building on the lab’s previous work, Sakuma et al. describe a detailed protocol for MMEJ-mediated knock-in...PITCh vector via In-Fusion or SLIC cloning. Step 2: Design an insertion locus-specific gRNA The gRNA should...binding in mammalian genomes as assessed by CRISPR design tools at crispr.mit.edu. They tested their system...

  18. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    .... Individual gRNAs are designed in silico using publicly available gRNA design software and synthesized... be independently validated to ensure that the desired modification produces the phenotype you screened...in the first place. With the proper experimental design and validation practices, CRISPR libraries can ...CRISPR Plasmids Read our CRISPR Guide Find tools to design your gRNA ...

  19. Synthetic Biology - Assembly Standards Guide

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    Collection
    ...after the promoter, and so on. The following table provides information for the assembly standards most commonly...will need to be reverse complimented for primer design. The restriction enzymes used for assembly are ..., NotI , PstI Scar: GCTAGT Features: 6 bp scar encodes Ala-Ser; Allows for in-frame fusions; NheI is rare...Enzymes: BamHI , Xhol Scar: GGATCT Features: Scar encodes Gly-Ser in-frame with prefix start codon; Employs...

  20. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...human therapeutics (e.g. using Sleeping Beauty). Despite their diverse utility, DNA transposons have been...(V. cholerae INTEGRATE - hereafter VchINT) and encodes a Type I-F CRISPR-Cas system. The four major components...the same promoter, and a donor plasmid (pDonor) encodes the mini-transposon. pEffector and pDonor can also...: Clone spacer(s) into the CRISPR array Clone desired DNA cargo into the mini-transposon Transform plasmid...even very large genetic cargo, at one or multiple desired genomic loci. Many applications of these systems...
Showing: 341 - 360 of 993 results