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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 660 results for: EPO

Showing: 341 - 360 of 660 results

  3. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...system, Ye’s lab could edit a tdTomato fluorescent reporter gene in mice using Minicircle DNA vectors encoding...seen in the figure below. Next, by using a dual reporter fluorescence assay, Hemphill et al. demonstrated...

  4. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    .... (2013). One-Step generation of mice carrying reporter and conditional alleles by CRISPR/CAS-Mediated...Addgene.org CRISPR guide CRISPR protocols by Addgene depositors gRNA design software CRISPR plasmid collections...

  5. Luciferase Plasmid Collection

    Type
    Collection
    ... 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic ...Multiple Multi-luciferase reporter vector including five transcriptional reporters for NF-kb, TGF-b, c-Myc...for gene expression assays and bioluminescent reporters. Plasmid...Collection Empty Backbones Expression Constructs Reporter Constructs Other Highlights You may also like....-lives, making them useful as transcriptional reporters, but are less-than-ideal protein tags due to their...or gene of interest into to create a luciferase reporter. Unless otherwise noted, these plasmids are meant...luciferase and renilla_polyA Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions...

  6. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...68289 SepOTSθ Plasmid 68288 SepOTSη Plasmid 68287 SepOTSζ Plasmid 68286 SepOTSϵ Plasmid 68285 SepOTSδ Plasmid...Plasmid 68294 SepOTSν Plasmid 68292 SepOTSλ Plasmid 68291 SepOTSκ Plasmid 68290 SepOTSι Plasmid 68289 ...Plasmid 68284 SepOTSγ Plasmid 68283 SepOTSβ Plasmid 53225 MBP-MEK1 S218TAG/S222TAG ( aka. PCRT7 tetR pLtetO...EcAR7 Plasmid 52054 SepOTSµ ( aka. B40 OTS) Strain 34929 BL21ΔserB Plasmid 34624 SepOTSα tRNA-Sep ( aka. ... Depositor Collections Rinehart Lab Phosphoprotein Reagents...#188537) a better choice in place of the popular SepOTSλ (Plasmid #68292) . An important technical note:...optimized phosphoserine orthogonal translation system, SepOTSλ (Plasmid #68292) , they were able to demonstrate...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation process by a novel reporter protein... DsRed1-E5 as a reporter for mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial...Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters via electrochromic...genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes A turquoise...Calcium Ratiometric calcium biosensors from nested reporters of green- and orange-emitting proteins Ratiometric...imaging (Perceval) A genetically encoded fluorescent reporter of ATP:ADP ratio. Nat Methods. 2009 Feb . 6(2)...

  11. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...celebrate Addgene without recognizing our incredible depositors and requestors. Thank you all so much for an ...

  14. Addgene at SfN23!

    Type
    Blog Post
    ...curation manager, Angela, experts on the Addgene repository, are also attending! Finally, our team is rounded...

  16. Bacterial Expression Systems

    Type
    Collection
    ...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events...proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161...protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Visualization Protein Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also like…...bacterium. Plasmids containing easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent ...transcription factor to bind to a small molecule, the reporter plasmid can be used to detect the presence of ...it. Bacterial one-hybrid (B1H) systems also use reporter genes to identify interactions between proteins...

  18. Call for Guest Bloggers

    Type
    Blog Post
    ...for scientists A new plasmid tool your lab has deposited If you’d like to write about a different topic...
Showing: 341 - 360 of 660 results