We narrowed to 706 results for: ESC;
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TypeBlog Post...plasmid encodes a green fluorescent protein gene which makes the bacterium fluoresce green. Comic by Maya...
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Improved Plasmid Maps Powered by SnapGene
TypeBlog Post...differ by only a few nucleotides, such as the fluorescent proteins EGFP and mEGFP. Many of these features...the gene, the name of its gene product, and a description of its function. You can also click one location... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post... Lab’s recent Nature Biotechnology paper, they describe TurboID and miniTurbo, which utilize non-toxic... money, and viruses at low concentrations often escape detection. The Alexander Green lab found a solution... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post... NEB's Resource describing Dam and Dcm Methylases Discovery of DpnI Review describing dam-/dcm- E. coli... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog Post...immediately write a text document with a brief description of the directory contents, at a minimum. This...for a single use, save the script as originalName_descriptionOfEdit in the same directory where it was ... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post...diverges from previously discussed methods. As described above, you design two primers, each containing...vitro recombination. Across all modifications described in Trehan et al., REPLACR-mutagenesis’ median ... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog Post... for over 10 years. Many successful scientists describe having a “posse” of mentors as one key to their...Ellen Daniell. In “Every Other Thursday,” Daniell describes a group of 7 women scientists who met every other... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog Post...mentoring questions I set out to answer. First, I will describe some of the many ways you can approach finding...Watch for my upcoming mentoring post in which I describe how to organize a successful peer mentoring circle... -
Antibodies 101: Fab Fragments
TypeBlog Post...talking about here? Many of the Fab fragments described above can be used for similar applications and...tissue-specific molecules identified by a double-label immunofluorescence technique for monoclonal antibodies. J Histochem... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post..., non-dividing cells. Figure 2: DNA rescue and transduction of a conventional single-stranded...Full-length ssAAV vector genome of both polarities are rescued from the vector plasmid and individually packaged... -
All in a Twist: dsRNA
TypeBlog Post...prone to degradation – those are the words that describe RNA! Double stranded? Well, that’s just for DNA... and repetitive sequence. All of the R-loops described above play distinct roles in promoting cellular... -
Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog Post...simple LED light. Lighting up your gRNA with fluorescent dyes In a scientific world where it often seems...seems like everything glows, why not spread the fluorescence to your gRNAs? Dye labeling the RNA in your ... -
Transferable Skills Guide: Problem Solving
TypeBlog Post...molecular biology experiments was in the job description, but this skill is not just about reacting to...research non-academic careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...in HeLa and MEF cells. For further reading on fluorescent microscopy techniques, the first author of this...El-Samad lab has deposited plasmids encoding fluorescent reporters and other proteins controlled by GAL4... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...Systems Biology in November, Voigt and Alex Nielsen describe what’s possible when multi-input CRISPR/Cas genetic...many deposits to Addgene over the years. Can you describe the toolbox you are putting together? Voigt: ... -
CRISPR in the Clinic
TypeBlog Post...potential for treatment is no longer an accurate descriptor; progress in clinical therapy is more fitting...They found that the cataracts phenotype could be rescued by zygote co-injection of Cas9 and a single-guide... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...efficient. Informed by these mutations and previously described TadA mutations (Gaudelli et al., 2017), they modified...undesired off-target editing effects. ABEs have been described to introduce base changes in cellular DNA and ... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...dividing and non-dividing cells. More detailed descriptions of the lentiviral elements and the 2nd and 3rd... deletion (Cre), together with expression of fluorescent or enzymatic reporters for cellular assays and... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...A recent publication [1] from the Sánchez lab describes the plasmids and protocols required for both WISH... which are available through the deposit) are described in the Sánchez lab publication [1] so that the... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-GFP). The lab deposited AAV...promoter to drive expression of a Cre-dependent fluorescent CatCH-GFP reporter. Expression of GFP will only...