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We narrowed to 637 results for: ESP

Showing: 341 - 360 of 637 results

  1. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ... and these are all symptoms of a negotiation, especially when money is involved. However, it doesn’t have...how much do you need to start your lab”. You can respond by saying, “The exact start up costs may vary greatly...

  3. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...cardiac, and retinal cells (Issa et al., 2023). AAV2 Despite being isolated second, AAV2 is the best studied...expression, and can cross blood vessel barriers. It is especially popular for applications targeting liver tissue...

  4. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ... difficult to quantify accurately, but can be especially helpful to generate more signal from low abundance...the multicolor overlay; this helps all viewers, especially if you have some complex patterns with several...

  6. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...allows you to easily orient your gene insert with respect to the promoter. For example, let’s say your plasmid...being made (post or pre run in the table above, respectively). Some of the above stains require you to visualize...

  8. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ... lab Undergraduate student Roles and responsibilities can vary "Lab assistants" usually wash glassware... my experience in the lab? Ask your questions—especially if it’s scary. I’ve been afraid to ask “dumb ...

  10. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...validation techniques for academia, industry, and especially the clinic. Popular validation assays are insufficient...search the whole genome for off-target sites irrespective of in silico prediction. These techniques differ...

  11. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...lower than the energy of the absorbed photon. Correspondingly, wavelengths of emitted photons are higher ...FRET).   Figure 1: Emission spectra and respective colors of a few commonly used fluorophores. Each...

  13. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ... of a pathway to inhibit to block the cellular response to a particular ligand (such as when a component...pathway. For example, one could study the cellular response to a cytokine known to cause the up-regulation...

  14. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...through the excitation light path. Each hole corresponds to a different location in the sample. As more...step that reassigns out of focus light to its respective focal plane. In sufficiently thin samples, deconvolution...

  15. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...mastered this technique so it might be worth a try, especially since the potential rewards could be substantial...) distance at which 50% FRET occurs. b Range corresponds to calculated D/A distances for observed FRET...

  16. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...bricks” to be added one by one. This approach is especially useful for combinatorial analyses. For example... L-arabinose and anhydrotetracycline (aTet), respectively. pMAZ-SK contains an aTet-inducible gRNA and...

  17. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...labelled with a green or violet, fluorescent dye, respectively. WT and KO cells were mixed in a 1:1 ratio and...the indicated Synaptotagmin-1 antibodies and corresponding Multi-rAb CoraLite® Plus 647 secondary antibodies...

  19. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ... to de-differentiate. However it’s difficult, especially as a newbie reprogrammer, to decide which method...reprogramming transgenes and the up-regulation of corresponding endogenous pluripotency genes. Retroviral vectors...

  20. Deep Dive: qPCR

    Type
    Blog Post
    ... peak indicating 2 amplicons. Image credits (respectively): Zuzanna K. Filutowska; Selma, 2019; Trampuz...you’re using a different brand of qPCR machine, especially if you’re using a rapid-cycle machine such as...
Showing: 341 - 360 of 637 results