We narrowed to 671 results for: FIE

...During the 2003 SARS outbreak, Hyeryun Choe’s lab identified that ACE2 is the functional receptor for SARS-CoV...expressing SARS-CoV-2 open reading frames and identified interactions of these proteins with human proteins...

... few patterns throughout the project where it solidified some concepts that are heard often during undergrad...dreaded coming to work because I truly enjoy the field I am in, the atmosphere, and the people.” My absolute...

...synapse from one cell to another, it must replicate. Modified Rabies Virus: Converting a transsynaptic neuronal...first-order connections to the starter cell, RABV was modified to only infect user-defined starter cell types...

...to select for successfully modified zygotes or embryonic stem cells. Modified embryonic stem cells are ...

...into science communication are as varied as the field itself. Among other things, science communication...blog that I started writing three years ago on a field trip in Indonesia. Since then, I have tried many...

...questions, data, and conclusions should be clearly identified and interpreted in less than a couple of minutes...poster will naturally sit outside of the reader’s field of view because of its length, and will be more ...

...bud into the cell culture supernatant and can be purified by centrifugation. Gag-Cas9 fusion protein The...is incorporated into Nanoblades, just like the unmodified MLV Gag protein. After Gag-Cas9 is incorporated...

...is to collaborate with specialists in different fields that can provide different insight into an area...your work and possibly a new perspective on the field. 3. Highlight your network and your collaborators...

...on or continue their careers in science-related fields. In particular, two recent events have the potential...Workshops will be run by experts in their respective fields: David Cameron, PhD - Director of Media Communications...

...viral RNA is first converted to cDNA and then quantified using qPCR primers targeting specific viral components...with the antibiotic, and colony-forming units quantified. While these methods tend to be more accurate...

...anhydrous tetracycline. Three new anti-CRISPRs were identified that inhibit the S. aureus Cas9, a Cas protein...easier to deliver, but so far has few inhibitors identified.     New from the viral service By Jason Nasse...

... composed of three proteins that can be easily modified in order to facilitate its entry into the target... AdV donor DNA leads to a vast majority of AdV-modified human cells being subjected to scarless homology-directed...

...will depend on the virus type. AAV is typically purified using methods like ultracentrifugation, while ... P0), infecting new Sf9 cells, and getting an amplified P2 virus. This P2 virus can be produced in a greater...

.... CRISPR/Cas9 technology has revolutionized the fields of molecular biology and bioengineering, as it ...eliminated (Fig. 1) (1, 3). The gRNA can be further modified by incorporation of an RNA hairpin, such as the...

... Classification of stem cells Stem cells are classified into two broad types according to their source...Kotton Four Factors that Differentiate the Stem Cell Field Resources on Addgene.org Visit Our Stem Cell Page...

...plasmids (and libraries)? We wanted to know, so we classified CRISPR plasmids containing a nuclease into three...make a precise edit or insertion via a chemical modifier. We used these categories to see what kinds of...

...using a simple PNK reaction or can be purchased 5’-modified from a company. However, unlike an RNA guide, ...your favorite model system, but the genome-editing field is advancing rapidly and it wouldn’t be a surprise...

...our older plasmids may not have an entry in this field. If you’ve deposited a plasmid without a purpose...Backbone: The starting vector that the depositing lab modified to create their new plasmid.  The Addgene Vector...

... protein, RNA, or DNA. Finally, once you have identified the best expression system, you can then choose...embryos. Cas9-gRNA ribonucleoprotein complexes Purified Cas9 protein and in vitro transcribed gRNA are...

...developed using a stereomicroscope to capture large field-of-views (6). For more comprehensive reviews on ... alcohol and methyl salicylate which was later modified by others to produce Murray’s clear solution.  ...