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  2. 6 Tips for Grant Writing

    Type
    Blog Post
    ...is to collaborate with specialists in different fields that can provide different insight into an area...your work and possibly a new perspective on the field. 3. Highlight your network and your collaborators...

  4. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ... use in mice and human brain IHC and uploading verified antibodies onto the mAb3D atlas website. This ... into expression constructs to be conveniently modified and engineered.   Fig. 1: Antibody production...

  9. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...viral RNA is first converted to cDNA and then quantified using qPCR primers targeting specific viral components...with the antibiotic, and colony-forming units quantified. While these methods tend to be more accurate...

  11. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    .... CRISPR/Cas9 technology has revolutionized the fields of molecular biology and bioengineering, as it ...eliminated (Fig. 1) (1, 3). The gRNA can be further modified by incorporation of an RNA hairpin, such as the...

  12. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...will depend on the virus type. AAV is typically purified using methods like ultracentrifugation, while ... P0), infecting new Sf9 cells, and getting an amplified P2 virus. This P2 virus can be produced in a greater...

  13. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...plasmids (and libraries)? We wanted to know, so we classified CRISPR plasmids containing a nuclease into three...make a precise edit or insertion via a chemical modifier. We used these categories to see what kinds of...

  14. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...anhydrous tetracycline. Three new anti-CRISPRs were identified that inhibit the S. aureus Cas9, a Cas protein...easier to deliver, but so far has few inhibitors identified.     New from the viral service By Jason Nasse...

  16. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...using a simple PNK reaction or can be purchased 5’-modified from a company. However, unlike an RNA guide, ...your favorite model system, but the genome-editing field is advancing rapidly and it wouldn’t be a surprise...

  17. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...our older plasmids may not have an entry in this field. If you’ve deposited a plasmid without a purpose...Backbone: The starting vector that the depositing lab modified to create their new plasmid.  The Addgene Vector...

  19. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...developed using a stereomicroscope to capture large field-of-views (6). For more comprehensive reviews on ... alcohol and methyl salicylate which was later modified by others to produce Murray’s clear solution.  ...
Showing: 341 - 360 of 665 results