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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 856 results for: HA

Showing: 341 - 360 of 856 results

  1. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...time in grad school. As you'll learn, grad school has its ups and downs for everyone but you can come out...their grad student communities 5:59 - 8:16 : What has made you unhappy in grad school? As part of this ...

  2. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...repository, and getting feedback from our community. It has been great to hear all the words of support from ...from scientists using Addgene in their labs. And it has been exciting to learn about all the amazing research...

  3. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...of just how big and mainstream the news on CRISPR has gotten, consider these headlines in The New York ...New York Times feature when he said, “All of this has basically happened in a year. It’s incredible.” ...

  4. Pipetting Protocol

    Type
    Protocol
    ... flow rate into the pipette tip. Once the liquid has finished drawing into the pipette tip, lift the pipette...

  6. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...in vivo scientists need a protease reporter that has a large dynamic range and high brightness. The Shu...in the midgut of Drosophila, an observation that has been difficult until now (Zhang et al., 2019). Conclusion...Conclusion FlipGFP is a novel fluorogenic reporter that has a large dynamic range and high brightness. This reporter...

  7. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...Adenovirus is a linear, dsDNA virus naturally. It has ITRs, just like AAV, which encompass the expression...packaging and entry Adenovirus is quite large and has more helper plasmid components than the other viruses...of adenovirus Building viral vectors Addgene has many viral vectors and helper plasmids for all the...

  9. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...15 years of plasmid sharing has certainly taken us on many adventures...From moving office locations ...plasmids are from the Zhang lab. In fact, the Zhang lab has shared over 40,000 CRISPR-Cas9 components over the...Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9) has been widely popular in genome engineering. For use...

  10. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...restricted based on experimental constraints. This has been used for a wide range of purposes, including...Cre-containing adenovirus (Ad-Cre) or AAV (AAV-pgk-Cre) has been used to successfully introduce Cre into cells... can be an effective alternative to Cre-lox, and has also been used in conjunction with it, allowing for...

  11. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...in bacteria, most CRISPR-based genome engineering has taken place in other organisms. In many bacteria,... targets cells in which homologous recombination has not occurred. This powerful negative selection system... works similar to the original pCas/pTargetF but has many other advantages including shorter plasmid curing...plasmids Noting that the more common spCas9 system has generally low efficiencies or can be proteotoxic ...system can be delivered a number of ways but success has been observed with Phagemids (Selle et al., 2020,...microbiome Beyond the human gut, the Barrick lab has deposited plasmids that can be used to engineer the...

  12. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ...Henry Bourne, Professor Emeritus at UCSF. Dr. Bourne has some radical (and some not so radical) suggestions...called The Balsa Group. University of Pennsylvania has the Penn Biotech Group and PBG Healthcare Consulting...

  13. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...and you need the right experimental materials. Who has time for paperwork? Here’s why a Material Transfer...confidence. Benefits of the UBMTA: Peace of mind Addgene has adopted the terms of the Uniform Biological Material...

  14. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...effective than both AAV9 and AAV5, which has tropism for lung cells, and has increased overall transduction throughout...this definition: A systemic capsid is a capsid that has been engineered to have enhanced function after intravenous...both). Therefore, a high-efficiency systemic capsid has better efficacy than AAV9 and/or its intermediate...used in LY6A-nonpermissive mouse strains. **AAV1-X1 has not been tested in rats. For more information on ...neurons in the CNS (Chan et al., 2017). AAV-PHP.eB has frequently been used for screening candidate regulatory...depositing manuscript) across the brain. AAV-BI-28 has been tested in C57BL/6J mice and effectively transduces.... 4c, Jang et al., 2023; Brown et al., 2021.) It has a similar transduction rate to AAV-PHP.eB in the ...

  15. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ... to its unrivaled contrast, fluorescence imaging has emerged as the dominant light microscopy contrast...blue to excite a molecule of GFP. Because red light has approximately half the energy of blue light, two ... be establish for this to happen.Using red light has a second advantage: red light penetrates deeper into...imaging depths closer to 1 mm. Traditionally, 2-photon has been a very slow imaging technique but recent advances... modern explosion in light microscopy techniques has provided biologists with a host of tools and opened...

  16. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ... cell mobility within these complex environments has never been easy and, for many years, researchers ...Intravital imaging powered by two-photon microscopy has uncovered host-pathogen interactions leading to the...proteins can be used in your experiments, experience has shown that some are more reliable and more efficient...important to finely tune the excitation wavelength as it has been shown that even a small incremental change of...this system called Brainbow, the Ubow mouse strain has been created to fate map Langerhans cells and follicular...

  18. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...all four parts of a STAR answer once the interview has started. Instead, I preferred to prepare stories ...stories for an interview, since one story usually has more than one theme and can be used to answer multiple...new idea to the company’s area of expertise. This has potential to make your audience really sit up and...

  20. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ... a lot quicker to pick up new languages!) Jargon has a place in science communication, but the more you...length of your average PhD to achieve!) Each field has its own framework: a particular way of organizing... amount of knowledge and expertise your audience has is a major factor in how quickly they can understand...
Showing: 341 - 360 of 856 results