We narrowed to 433 results for: Hal

1. Plasmids for Endogenous Gene Tagging in Human Cells

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   Blog Post

   ...Institute website at alleninstitute.org. A classic challenge in cell biology is making sure that what we observe...

2. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   ... (budding yeast), S. pombe (fission yeast), A. thaliana (mustard weed), O. cuniculus (rabbit), synthetic...

3. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   ...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different...

4. Hot Plasmids and Viral Preps - January 2021

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   ... is only ~70kDa, with a molecular weight roughly half of Cas9 or Cas12a. This is in part due to the fact...

5. Adapting Toehold Switches to Detect Zika Virus

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   ...symptoms, including the development of fetal microcephaly and Guillain-Barré syndrome. In response, the...

6. The Materials Science of Optogenetics Experiments

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   ...validation experiments we discussed last week are only half of the battle when using optogentics to answer a...

7. Experimenting in an Open Source lab: from CRISPR to Cats

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   ... case.   Funding Makerspaces One of the main challenges when starting a new project at a makerspace is...

8. Hot Plasmids: Winter 2025

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   ... developed in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots....

9. The AAVantages of AAV in CRISPR Screens

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   .... V., Teyssier, N., Pan, A., Danner-Bocks, S., Sanghal, T., Yadanar, L., Tian, R., Ma, K., Palop, J. J...

10. Production of Virus in Insect Versus Mammalian Cells

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    ...Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production Tips for Titering Your...

11. Antibodies 101: Flow Compensation

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    ...Compensating between your fluorescent markers can be challenging! But we hope this guide can help you get started...

12. Twenty Years of Addgene Sharing: CRISPR

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    ... the easy part; using them can be a bit more challenging. Addgene’s free educational resources help scientists...

13. Academic vs. Industry Postdocs

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    ...in-depth discussion about your project might be challenging. In an academic environment, surrounded by lab...

14. Plasmids 101: 5 factors to help you choose the right cloning method

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    Blog Post

    ...technologies currently available, you now face the challenge of trying to figure out what system might work...

15. Transferable Skills Guide: Managing a Team

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    .... Personally I found this to be particularly challenging. As PhD students and postdocs we are used to ...

16. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    ...access to routinely, and it can be especially challenging when trying to develop antibodies against unstable...

17. Truncated gRNAs for Regulating Gene Expression

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    ...different perturbations. Consider, for instance, the challenge of attempting to simultaneously and selectively...

18. PCR Applications for Copy Number Variation Assays

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    ...available, ddPCR may be a better option for more challenging cases.  Price point If budget is a concern...

19. Polymerase Chain Reaction Overview and Applications

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    ...standard curve, which can be time consuming and challenging to get right. ddPCR uses a water-oil emulsion...

20. Of Myc and Men

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    ...in these species, they found that the N-terminal half of the antigen differs significantly between human...