We narrowed to 416 results for: Numb

1. Overcoming the AAV Size Limitation for CRISPR Delivery

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   ...requires a very specific PAM sequence that limits the number of targetable loci, and truncated Cas9 has much...

2. CRISPR-mediated Plant Base Editors

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   ...base editor platforms. Hua et al. constructed a number of plant base editors replacing the SpCas9 with...

3. How to Write a Scientific Review Article

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   ... thing I did was stare in horror at the limited number of hits. So, here, I will share my process with...

4. Plasmids 101: Gateway Cloning

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   ...fits your experiment. This choice will depend on a number of factors, like your organism, desired expression...

5. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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   ...cells in real time. Previously, we have described a number of practical considerations that should be taken...

6. Plasmids 101: E. coli Strains for Protein Expression

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   ...expression strain? Protein expression from high-copy number plasmids and powerful promoters will greatly exceed...

7. Tips for Using FRET in Your Experiments

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   ... will be fixed. The quantum yield of the donor (number of emitted photons per absorbed photons) and the...

8. Plasmids 101: Expressing shRNAs for RNA Interference

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   ...delivery of a plasmid. RNAi is an umbrella term for a number of different applications that involve different...

9. CRISPR 101: Homology Directed Repair

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   ... for each homology arm, but keep in mind these numbers may vary based on your locus of interest and experimental...

10. Quick Guide to Near-Infrared Fluorescent Proteins

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    ...of spectrally distinct (e)miRFPs available. The numbers in (e)miRFP names correspond to the maxima of their...

11. March for Science

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    ..., but data is not going change depending on the number of people supporting it.  3) Linking scientists...

12. Harnessing Bacterial Toxins for Allelic Exchange

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    ...postdoctoral researcher at Harvard. There’s an astounding number of ways to create chromosomal mutations in bacteria...

13. Five Popular Model Organisms

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    ...life cycle of 8 to 14 days, and produce a large number of offspring that are laid externally. Fruit flies...

14. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    ...addition, it increases avidity because it doubles the number of binding sites per unit.  There are several ways...

15. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    ...versions have been developed, which differ in the number, stability, and brightness of the fluorescent proteins...

16. Five Popular Model Organisms, Part 2

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    ...ideal model organism: quick development, a large number of offspring (produces many seeds), self fertilizing...

17. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    ...by the top strand. Both produced about the same number of positive clones. See Supplementary Note 1 in...

18. Using Phosphoserine to Study Protein Phosphorylation

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    ... methods for studying protein phosphorylation A number of classic methods exist for this, the most common...

19. Evolution of Lab Techniques

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    ...in situ hybridization for detection of gene copy number changes." Protocols for Nucleic Acid Analysis by...

20. Treating Muscular Dystrophy with CRISPR Gene Editing

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    ...excited. This approach may also be applicable to a number of rare genetic diseases caused by splicing defects...