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...requires a very specific PAM sequence that limits the number of targetable loci, and truncated Cas9 has much...

...on for your best options. There are an expanding number of Cas9s with looser PAM requirements compared ...

...dropped” in the wrong place, while increasing the number of potential applications. But they weren’t done...

....  PE7 shows enhanced editing efficiencies in a number of cell types at different genomic loci — in some...

...TTAT-3′). The unique TAM requirements expand the number of genomic sites that can be targeted with TnpB...

...procedure to improve throughput by increasing the number of reactions. The main steps of short-read sequencing...

... bacteria in favorable conditions double their numbers rapidly. The knowledge the Singletons gained from...

...cells in real time. Previously, we have described a number of practical considerations that should be taken...

...expression strain? Protein expression from high-copy number plasmids and powerful promoters will greatly exceed...

... will be fixed. The quantum yield of the donor (number of emitted photons per absorbed photons) and the...

...transfection. Test a variety of brands and FBS lot numbers to find one suitable with your protocols. FBS can...-Tip Transducing too many cells relative to the number of virus particles reduces the transduction efficiency...massive cell death upon antibiotic selection. The number of cells transduced should be enough that they ...amount of time, but not so many that they vastly outnumber the virus particles. Incubate the cells with the...lentivirus used, cells may also exhibit varying numbers of integration events. In other words, if an MOI...

...constructed a plasmid, you can easily make an endless number of copies of the plasmid using bacteria, which ...BL21 and its derivatives. We have included a small number of E. coli strains below and recommend checking...

..., but data is not going change depending on the number of people supporting it.  3) Linking scientists...

...by the top strand. Both produced about the same number of positive clones. See Supplementary Note 1 in...

...postdoctoral researcher at Harvard. There’s an astounding number of ways to create chromosomal mutations in bacteria...

...life cycle of 8 to 14 days, and produce a large number of offspring that are laid externally. Fruit flies...

...addition, it increases avidity because it doubles the number of binding sites per unit.  There are several ways...

...versions have been developed, which differ in the number, stability, and brightness of the fluorescent proteins...

...ideal model organism: quick development, a large number of offspring (produces many seeds), self fertilizing...

...of spectrally distinct (e)miRFPs available. The numbers in (e)miRFP names correspond to the maxima of their...