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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 566 results for: PAC

Showing: 341 - 360 of 566 results

  2. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...Furthermore, piggyBac™ offers a large cargo-carrying capacity (over 200 kb has been demonstrated) with no known...certain genes, such as those that have negative impacts on fitness. One consideration when making a transgenic...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Addgene has an extensive collection of plasmids for packaging and expression, see our dedicated Adeno-associated...Addgene has an extensive collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids...

  6. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ... epigenetic modification, bulges) that may also impact off-target edits. As a result, only sequencing ...algorithms may not provide a full picture of the impact of CRISPR editing in the model cell line or organism...

  7. Zhang Lab CRISPR Page

    Type
    Collection
    ...- Cas9 Mouse CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a microbial ...SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene and its single guide RNA ...

  8. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...distance in the placement of plasmid elements can impact expression levels and consequently convolute the... different lentiviral plasmid backbone elements impact viral titer and to compare different Cas enzymes...

  9. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...populations that are the most likely to be positively impacted by the trial. During clinical trials, expansion... strategies that synergize with these positive impacts on the immune system will likely have better treatment...

  11. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...–6 67,366 (Set A) 67,194 (Set B) Cas13a/C2c2 Protospacer flanking site (PFS) Library 79153 Knockout E....E. coli Zhang N/A N/A N/A - The protospacers contained in the library represent all 4096 (4 6 ) combinations...

  12. Plasmids for Stem Cell Research

    Type
    Collection
    ...are proliferative, unspecialized cells with the capacity to differentiate into many different cell types...every other cell type in the body and have the capacity to give rise to an entire organism. iPSC technology...

  13. Tetracycline Inducible Expression

    Type
    Collection
    ...transactivators and inducible tools or consider our AAV Packaged on Request service available for many transactivator...H., & Hillen, W. (2000). Exploring the sequence space for tetracycline-dependent transcriptional activators...

  15. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Bruchez 45944 pTDpelB-C_sfYFPTwinStrep Periplasmic space PelB signal sequence YFP Thorben Dammeyer 54520 ...transported to the Golgi apparatus in vesicles to be packaged and shipped. Many of the proteins that enter the...

  16. Deep Dive: qPCR

    Type
    Blog Post
    ...multiplex your reference genes to save on plate space. For many cell lines and tissue types, appropriate...your reference gene set can save significant plate space and may be worth the initial effort of setting it...

  17. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...combination of all 11 att pairs in series with unique spacer sequences (pMemoryArray) gives a DNA sequence capable...compartment (e.g. the plasma membrane, the extracellular space, or the lysosomes) can take many paths. Understanding... to other viral systems due to its large cargo capacity, minimal cytotoxicity, and broad host-tropism....

  18. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... Gateway-Compatible Toolbox Podcast Segment The impact of KLF4 N-terminal variants on iPSC generation ... expression levels; this altered stoichiometry impacted reprogramming and global gene expression patterns...

  19. Antibody Guide

    Type
    Collection
    ...antibodies are collected, tested against the antigen, packaged, and sold. This is the most cost-efficient way...antibodies to upwards of twenty. Laser and software capacity theoretically allow for roughly fifty different...
Showing: 341 - 360 of 566 results