We narrowed to 653 results for: PPL
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TypeBlog Post... is almost always worth it. Because labels are applied to containers and not the mushy, gooey contents...be!) bear in mind that your barcodes are being applied to the containers, not to the contents. Tightly...
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Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog Post...derived from S. aureus. This means you can likely apply similar rational mutations to your Cas9 homolog ...may decrease worries about off target effects in applied and/or therapeutic settings. Will combining the... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...were so many interesting questions that we could apply [it to].” Using ClonTracer to study drug resistance...Novartis Oncology and is particularly interested in applying what we have learned about tumor heterogeneity... -
Plasmids 101: Golden Gate Cloning
TypeBlog Post...engineering fields. We’ll walk you through how to apply this precise and easy-to-use system to your cloning...different combinations, or other new and exciting applications, this system is an incredibly powerful tool ... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...potential to be used for other types of CRISPR applications. Let’s take a look at the key components of ...After Gag-Cas9 is incorporated, the MLV protease, supplied as part of the MLV Gag-Pol polyprotein, cleaves... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog Post...that can be used to simulate muscle tissue as it applies to C. elegans locomotion. To see the other resources...biology in C. elegans." Nucleic acids research 36.suppl_1 (2007): D57-D62. PubMed PMID: 17986455. PubMed ... -
Engaging with science and society at pgEd
TypeBlog Post...latest developments in genetics research, their applications and regulatory policies; keeping tabs on the...and who will be affected by the knowledge and applications generated from this research. At both a practical... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...Glycoimmunology group based at the Research Unit on Applied Molecular Biosciences (UCIBIO) focused on understanding...different Arg-mutant enzymes could be rescued by supplementation with guanidine hydrochloride (GdnHCl). Rescue... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post...of xanthine into guanine, and without xanthine supplied in the media, these bacteria are unable to divide...was able to grow this strain in minimal media supplemented with various caffeinated beverages, but not ... -
How to Prepare for an Industry Interview
TypeBlog Post...noticed one of the biggest differences between applying for an academic position versus an industry position...interview is an opportunity to learn something and then apply it to the next interview. Perhaps an interviewer... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post...the co-op application process, my advisor encouraged me to keep my values in mind as I applied. Thinking... -
Plasmids 101: Modular Cloning
TypeBlog Post...multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits Most things in biology are...phenotype is best described with “it’s complicated.” Applying MoClo Interested in using MoClo? You don’t have... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...location of the cut and subsequent edit. In these applications, location takes priority over cutting efficiency...in double stranded cleavage and base editing applications, with high editing capacity in both contexts... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post...it comes to targeting precision and practical application. If the CRISPR/Cas9 system is not effective for...lncRNAs with high specificity, particularly in applications requiring smaller delivery systems (Zibitt et... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post... for lightning-mediated gene transfer in soil. Applied and Environmental Microbiology, 67(8), 3440–3444...Bacteria:Their Characteristics and Potential Applications in Biotechnology. Critical Reviews in Biotechnology... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog Post...focused on developing cell-free synthetic biology applications for human health and the environment. Alexander...nanomaterials science, and self-assembly for applications in health, energy, and sustainability. References... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post...eclipse any of the CRISPR nucleases in AAV-based applications. Get More Cpf1 Background Here THE ORIGINAL ...the PAM sequence. Many research and clinical applications require HDR over NHEJ, so comparing HDR efficiency... -
The Materials Science of Optogenetics Experiments
TypeBlog Post...the initial laser output. The same considerations apply to the polishing and preparation of the patch cable...Injectable, cellular-scale optoelectronics with applications for wireless optogenetics. Science. 2013;340... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post... in biology but don’t really know how to start applying their skills to it. The Kittybiome project gives...contributes each month - with only occasional supplementation with grant funds, money is always tight. Of... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog Post...and respond to novel substances. We both had an applied science bent, so the engineering aspect of this...about continuing with the business. We ended up applying to a business accelerator freely stating that ...