We narrowed to 651 results for: PPL
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TypeBlog Post...plasticware is readily available from scientific suppliers and glassware should be treated with a DEPC solution...potentially complicate results of a downstream application such as quantitative PCR to assess gene expression...
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Transferable Skills Guide: Cross-team Communication
TypeBlog Post...types or purposes of each given container, when applicable Any other relevant information Prescribing an...potentially ambiguous terms. Defining words and applying annotation can be particularly useful in academic... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...aggregates. This property can be tolerated in some applications (e.g. labeling of cells or tracking promotor...promotor activity), but it is problematic in applications in which the fluorescent protein is used as an inert... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post...reached at high power may not translate to real applications in which far less power is used. For example...insight into the photostability in the intended application (Goedhart et al, 2012). The third issue relates... -
CRISPRainbow and Genome Visualization
TypeBlog Post...gRNA binding a non-mammalian sequence (GAL4) was supplied. In addition to labeling repetitive telomeric...single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector. Figure 2: CRISPRainbow... -
Developing Lab Management Software for Biology
TypeBlog Post... is almost always worth it. Because labels are applied to containers and not the mushy, gooey contents...be!) bear in mind that your barcodes are being applied to the containers, not to the contents. Tightly... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog Post...derived from S. aureus. This means you can likely apply similar rational mutations to your Cas9 homolog ...may decrease worries about off target effects in applied and/or therapeutic settings. Will combining the... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...were so many interesting questions that we could apply [it to].” Using ClonTracer to study drug resistance...Novartis Oncology and is particularly interested in applying what we have learned about tumor heterogeneity... -
Plasmids 101: Golden Gate Cloning
TypeBlog Post...engineering fields. We’ll walk you through how to apply this precise and easy-to-use system to your cloning...different combinations, or other new and exciting applications, this system is an incredibly powerful tool ... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post...it comes to targeting precision and practical application. If the CRISPR/Cas9 system is not effective for...lncRNAs with high specificity, particularly in applications requiring smaller delivery systems (Zibitt et... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post... for lightning-mediated gene transfer in soil. Applied and Environmental Microbiology, 67(8), 3440–3444...Bacteria:Their Characteristics and Potential Applications in Biotechnology. Critical Reviews in Biotechnology... -
Plasmids 101: Modular Cloning
TypeBlog Post...multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits Most things in biology are...phenotype is best described with “it’s complicated.” Applying MoClo Interested in using MoClo? You don’t have... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...location of the cut and subsequent edit. In these applications, location takes priority over cutting efficiency...in double stranded cleavage and base editing applications, with high editing capacity in both contexts... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...potential to be used for other types of CRISPR applications. Let’s take a look at the key components of ...After Gag-Cas9 is incorporated, the MLV protease, supplied as part of the MLV Gag-Pol polyprotein, cleaves... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog Post...that can be used to simulate muscle tissue as it applies to C. elegans locomotion. To see the other resources...biology in C. elegans." Nucleic acids research 36.suppl_1 (2007): D57-D62. PubMed PMID: 17986455. PubMed ... -
How to Prepare for an Industry Interview
TypeBlog Post...noticed one of the biggest differences between applying for an academic position versus an industry position...interview is an opportunity to learn something and then apply it to the next interview. Perhaps an interviewer... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post...the co-op application process, my advisor encouraged me to keep my values in mind as I applied. Thinking... -
Engaging with science and society at pgEd
TypeBlog Post...latest developments in genetics research, their applications and regulatory policies; keeping tabs on the...and who will be affected by the knowledge and applications generated from this research. At both a practical... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...Glycoimmunology group based at the Research Unit on Applied Molecular Biosciences (UCIBIO) focused on understanding...different Arg-mutant enzymes could be rescued by supplementation with guanidine hydrochloride (GdnHCl). Rescue... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post...of xanthine into guanine, and without xanthine supplied in the media, these bacteria are unable to divide...was able to grow this strain in minimal media supplemented with various caffeinated beverages, but not ...