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  1. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...licensing restrictions. Use these instructions to prepare the PSU ladders! Rosado, et al. Autophagy. 2008...
  2. AAV Production in HEK293 Cells

    Type
    Protocol
    ... °C for 45 min. You are now ready to purify your prep . The clarified supernatant can be kept overnight...mM Tris HCl, 150 mM NaCl, 2 mM MgCl2) Reagent Preparation DMEM Complete : 10% v/v FBS and 4 mM L-alanyl-L-glutamine...et al., 2018 (Link opens in a new window) ). To prepare D1 + 0.1 M sorbitol, add 57 mL of 1.46 M sorbitol...
  3. AAVED

    Type
    Collection
    ...Melina Fan/Leila Haery 5:00 PM Adjourn Meeting Preparation For Speakers: Each speaker will give a 20 minute... reagents)? For All Attendees: To help us best prepare and compile resources for this meeting's publication...commitment to open science! Please see the meeting preparation section for how to best help contribute to this...
  4. Immunology Research Plasmids and Resources

    Type
    Collection
    ...LGR1, MGC141667, MGC141668, ODG1 GAL galanin prepropeptide GALN, GLNN, GMAP, MGC40167 GALP galanin-like...GHRFR, GHRHRpsv, GRFR GHRL ghrelin/obestatin prepropeptide MTLRP, obestatin GHSR growth hormone secretagogue...PGRN GRP gastrin-releasing peptide BN, GRP-10, preproGRP, proGRP GUCA2A guanylate cyclase activator 2A ... OSMRB OSTN osteocrin MUSCLIN OXT oxytocin, prepropeptide MGC126890, MGC126892, OT, OT-NPI OXTR oxytocin...pro-melanin-concentrating hormone MCH PNOC prepronociceptin PPNOC POMC proopiomelanocortin ACTH, CLIP,...
  5. Cre-lox system

    Type
    Collection
    ... of choice. We also offer ready-to-use viral preparations of select Cre expression plasmids. Addgene's...service provides high-quality and high-titer viral preparations of plasmids that are currently in our repository...
  6. Guide to Using Pooled Libraries

    Type
    Guide
    ... simplest level, pooled libraries are single preparations of many different plasmids. Plasmids within ...either case, next-generation sequencing of the maxiprep DNA is recommended to verify that the library ...process. Amplify the library (electroporation and maxiprep). If delivering as virus, make virus; this creates...
  7. Viral Vectors

    Type
    Collection
    ...array of research purposes. Ready-to-use viral preparations for select plasmids are now available through...
  8. COVID-19 Resources

    Type
    Collection
    ... Coronavirus Resource Hub COVID-19 SARS-CoV-2 preprints from medRxiv and bioRxiv Elsevier's Novel Coronavirus...
Showing: 341 - 360 of 388 results