We narrowed to 399 results for: PREP

1. CRISPR-based Genome Editing Systems For Editing in E. coli

   Type

   Blog Post

   ...and the BsaI linearized pEcgRNA backbone can be prepared in large amounts and frozen for future use or ...

2. Transferable Skills Guide: Career Planning Resources

   Type

   Blog Post

   ...of some of the resources you can use to better prepare for your career, and increase your chances of landing...

3. Career Insights: Technical Support Specialist

   Type

   Blog Post

   ... A degree in the life sciences prepares one for numerous non-academic careers. Still, many start their...

4. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

   Type

   Blog Post

   ...fluorescence visualizer with 9V battery Approach :This preprint uses a previously developed method to detect SARS-CoV...

5. New Optogenetic Tools for Cytoskeleton and Membrane Control

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   Blog Post

   ...7. https://doi.org/10.1038/s41557-023-01214-0. Preprint on bioRxiv: https://doi.org/10.1101/2022.09.26.509516...

6. Plasmids 101: Biotinylation

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   Blog Post

   ...Forster, A. C., Skingle, D. C., & Symons, R. H. “Preparation and uses of photobiotin.” Methods in Enzymology...

7. Transferable Skills Guide: Time Management

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   Blog Post

   ... can spend accomplishing small tasks (like... preparing plasmids to send to Addgene). Following my advice...

8. How to Negotiate a Successful Lab Start Up Budget

   Type

   Blog Post

   ...community is the ultimate value proposition. By preparing a budget before hand, you’re saving the institute...

9. A Control for All Seasons

   Type

   Blog Post

   ...antibody binding. For this control, samples are prepared following the standard protocol, but the primary...

10. Antibodies 101: The Basics of Western Blotting

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    Blog Post

    ... (SDS-PAGE) is the first step of a western. To prepare the samples for SDS-PAGE, measure the protein content...

11. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type

    Blog Post

    ... and here I summarize what I learned both in preparation for my talk and at the minisymposium. You can...

12. Generating Mouse Models Using CRISPR/Cas9

    Type

    Blog Post

    ...confused. It was supposed to be hard. I was mentally prepared to go through a learning curve that would result...

13. Finding Your Perfect Job After University

    Type

    Blog Post

    ...lacking during degree courses; they never really prepare you for the amount of failure and problem solving...

14. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    ...writing fellowships, giving oral presentations, and preparing for job interviews, expanded profesional networks...

15. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    Blog Post

    ... but it’s a very good practice for prevention. Prepare a small quantity of media stock that would be used...

16. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    ...Identity of Recombinant Adeno-Associated Viral Preparations from Viral DNA Extracts. Human Gene Therapy ...

17. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type

    Blog Post

    ...your work. Immediate steps that you can take to prepare for the green card process Update your CV and ...

18. Technical Design of a Western Blot

    Type

    Blog Post

    ...binding to your protein of interest. Protein preparation Before you can blot your proteins, you’ll need...

19. 15 Hot Plasmids from 2017

    Type

    Blog Post

    ...licensing restrictions. Use these instructions to prepare the PSU ladders! Rosado, et al. Autophagy. 2008...

20. AAV Production in HEK293 Cells

    Type

    Protocol

    ... °C for 45 min. You are now ready to purify your prep . The clarified supernatant can be kept overnight...mM Tris HCl, 150 mM NaCl, 2 mM MgCl2) Reagent Preparation DMEM Complete : 10% v/v FBS and 4 mM L-alanyl-L-glutamine...et al., 2018 (Link opens in a new window) ). To prepare D1 + 0.1 M sorbitol, add 57 mL of 1.46 M sorbitol...