We narrowed to 741 results for: REV
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TypeBlog Post...Cre-estrogen receptor ligand binding domain-fusion can prevent this toxicity, but with the caveat of partial rather...toxicity. FLP/FRT recombinases (from Saccharomyces cerevisiae) had been used successfully in Drosophila, so...
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Future of Research Conference - Remarkable Opening Session
TypeBlog Post...to control their own future in science (see my previous blog on this). Indeed, that was a primary message...rules and practices of indirect costs. The peer review and grant awards systems need to be radically overhauled... -
9 Tips to Achieve Success in Academia
TypeBlog Post...variety of careers outside of academia. She has had previous graduate students and postdocs go on to work in...what you are truly passionate about. Check out my previous post if you're looking for more information about... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog Post...lentiviral infection into target cells that have previously been stably transfected for Cas9 expression. ... in bacterial toxin resistance, identifying 4 previously unknown genes. “With this work, it is now possible... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...organism, all cells of that type are affected. Previous tools to overcome this restraint include an AAV-based...into an attB site. In the SPARC system, PhiC31 irreversibly recombines one of the two competing attP sites... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog Post...Since the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that...genome, thus providing an avenue for temporary/reversible CRISPR modification. In addition to these subpages... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog Post...Check Out Previous Addgene-Seeding Labs Plasmid Grant Awardees Listen to Our Interview with Previous Awardee... -
Build Your CRISPR Vocabulary
TypeBlog Post...bacterial chromosome that stores a genetic record of previously encountered foreign nucleic acids. The unusual...CRISPR–Cas immunity and genome editing enzymes. Nature Reviews Microbiology, 20(11), 641–656. https://doi.org/... -
Year of the Camelid: Antibody Style
TypeBlog Post...the Camelid family, I wanted to take a moment to revisit some of the ways these animals have impacted humans...Odongo S, Radwanska M, Magez S (2023) Nanobodies: A Review of Generation, Diagnostics and Therapeutics. Int... -
Antibodies 101: Affinity Tags
TypeBlog Post...and the species they are compatible with are all reviewed here. What are affinity tags? Affinity tags are...chromatography requires proper folding of the tag, which prevents insoluble fusion proteins from being purified ... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post... with inappropriate activin-ACVR1 interactions prevents FOP bone growth in mouse models. As this biologic...Resources on the Addgene Blog Learn about Addgene's Previous Work with Rare Disease Researchers Check Out Potentiall... -
RUBY-Red Siliques
TypeBlog Post...CRISPR construct needs to be segregated away to prevent continued production of new alleles or off-target... generation when CRISPR needs to be removed to prevent further editing, then green offspring can be selected... -
Antibodies 101: Epitope Availability
TypeBlog Post...other hand, if it opens up an epitope that was previously hidden, an antibody might suddenly work that ...denatured, allowing the antibody to bind to a previously hidden epitope (right). Created withBioRender.com... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post...visualizing, and documenting their procedures. Preventable errors in the design of cloning strategies set...related versions that have different properties. To prevent misidentification, we augment the database with... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post...cell via endocytosis. In our study, we aimed to prevent the uptake of AAV2 viral particles by our packaging... cells with the endocytosis inhibitor sucrose. Previous work by Stoneham et al. demonstrated that the ... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post... are also of practical interest since they’ve previously yielded valuable enzymes. Famously, Taq polymerase...PNAS. Two other thermostable Cas9 enzymes have previously been characterized: GeoCas9 from the Doudna lab... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog Post...Congress on Peer Review and Scientific Publication, where diverse approaches to peer review were discussed... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...context, it’s hypothesized that base removal helps prevent the invading genome from “recovering” its original...cannot switch guides. While this fidelity would prevent other small nuclear DNAs from binding NgAgo, it... -
Addgene's Top 10 Blog Posts of 2015
TypeBlog Post... need to get started using plasmids and the revolutionary CRISPR technology in their own labs. We’re overjoyed...new technologies that will bring about the next revolution in biological research. We’re incredibly happy... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog Post...Genetics and Hereditary Symbiosis" Physiological Reviews 32.4 (1952) 403-430. Pubmed PMID: 13003535. Lederberg...Bacterial Plasmids: A Proposal.”Bacteriological Reviews 40.1 (1976): 168–189. PubMed PMID: 16350226. PubMed...