We narrowed to 665 results for: SIM

...and design described above can be used to create similar donor plasmids for introducing tags into your gene...

...plasmid deposit process can be broken down into four simple steps: Send plasmid information to Addgene Material...the Deposit Spreadsheet is the quickest option. Simply download the spreadsheet and copy and paste your...

...their parents. Here’s how to cross mice, using a simple example with the goal is to generate mice that ...raised in the same environment and will harbor similar epigenetic modifications. Special breeding cases...

...and Sibernetic, a fluid mechanics simulator that can be used to simulate muscle tissue as it applies to ...

...CRISPR systems based on Cas9 were thought to be the simplest CRISPR systems and the easiest to adapt to genome...et al. suggest that AsCpf1’s off-target rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9...

..., Scientific American or National Geographic. Similarly, media fellowships from the British Science Association... Media Production at Imperial College London. Similarly, the University of Cardiff offers an MSc in Science...

... For some transgenes, transfection optimization simply is not enough to get a sufficient titer; this is...volumes of lentivirus routinely, you may opt for simply increasing the number of 10 cm2 dishes. While this...

...CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability... albino mice ranging from 11% to 100%, a level similar to other approaches.  Nanoblades can also be used...

...OpenWorm project. The main goal of OpenWorm is to simulate C. elegans as a computer model. When I first heard...genome. While C. elegans are often thought of as simple organisms , they have many levels of complexity...

...End point PCR, which is commonly referred to as simply PCR, is used for an array of different molecular... or pathogen identification. Scientists can simultaneously detect several different pathogens in one specimen...

...jbc.RA120.01289     Novel protein cage provides simple enzyme protection in yeast bioproduction pathways... a reaction pathway, the MPyV system provides a simple, efficient method for protecting a single enzyme...

... change across the membrane of cardiac cells. Similarly, neuronal signaling is carried out by action potentials...detailed below. Voltage Sensitive Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to...

...after transforming them into your cells, you can simply look for blue colonies to determine which cells...cost yet. However, many fluorescent proteins face similar limitations, and many labs are already using these...

...administered through the tail vein — many researchers will simply refer to these as “tail vein injections.” However...Intranasal instillation Intranasal instillation is very similar to its intratracheal counterpart, as both procedures...

...see if any other fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling... tools, they’re optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools.  Embracing...

...insect cells require demanding culture conditions similar to mammalian expression systems. Bacterial expression...expression. Protein expression in bacteria is quite simple; DNA coding for your protein of interest is inserted...

...vectors can be used to tag endogenous genes and simultaneously introduce a selection marker for screening ... CRISPR/Cas Plasmids - Protein Tagging. Beyond simply monitoring protein localization and activity, CRISPR...

...Compared to humans and mice, flies have a relatively simple genome. They have four chromosomes: the X (and ... phenotypes, check out the Classical Genetics Simulator website. One interesting thing to know is that...

...transfer plasmid. The good news is there’s a few simple ways to deal with this issue. One way is to grow...cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging PEI transfections...

... helper viruses like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication. The ...purify your rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of ...