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  2. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...CRISPR systems based on Cas9 were thought to be the simplest CRISPR systems and the easiest to adapt to genome...et al. suggest that AsCpf1’s off-target rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9...

  3. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...OpenWorm project. The main goal of OpenWorm is to simulate C. elegans as a computer model. When I first heard...genome. While C. elegans are often thought of as simple organisms , they have many levels of complexity...

  4. Common Injection Routes in Mice

    Type
    Blog Post
    ...administered through the tail vein — many researchers will simply refer to these as “tail vein injections.” However...Intranasal instillation Intranasal instillation is very similar to its intratracheal counterpart, as both procedures...

  5. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...see if any other fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling... tools, they’re optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools.  Embracing...

  6. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ... helper viruses like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication. The ...purify your rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of ...

  7. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ... change across the membrane of cardiac cells. Similarly, neuronal signaling is carried out by action potentials...detailed below. Voltage Sensitive Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to...

  8. PhD Applications After COVID

    Type
    Blog Post
    ... tends to go something like this:   However, as simply as it seems laid out above, there are many places...potential programs and universities stressful or simply not feasible. Through the virtual systems created...

  10. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...insect cells require demanding culture conditions similar to mammalian expression systems. Bacterial expression...expression. Protein expression in bacteria is quite simple; DNA coding for your protein of interest is inserted...

  11. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...End point PCR, which is commonly referred to as simply PCR, is used for an array of different molecular... or pathogen identification. Scientists can simultaneously detect several different pathogens in one specimen...

  12. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...jbc.RA120.01289     Novel protein cage provides simple enzyme protection in yeast bioproduction pathways... a reaction pathway, the MPyV system provides a simple, efficient method for protecting a single enzyme...

  15. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...transfer plasmid. The good news is there’s a few simple ways to deal with this issue. One way is to grow...cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging PEI transfections...

  16. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...vectors can be used to tag endogenous genes and simultaneously introduce a selection marker for screening ... CRISPR/Cas Plasmids - Protein Tagging. Beyond simply monitoring protein localization and activity, CRISPR...

  17. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...figure below). The double-MMEJ strategy looks very similar to HDR, but it is accomplished using much smaller... of MMEJ. This double MMEJ strategy looks very similar to HDR, but it is completed using much shorter ...

  18. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...over previous genome editing techniques is its simplicity and versatility. CRISPR/Cas9 consists of two ...Pooled lentiviral CRISPR libraries (referred to simply as “CRISPR libraries”) consist of a heterogeneous...

  19. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins from ...reporter cloned in. In these plasmids you would simply clone your gene of interest into the multiple cloning...
Showing: 341 - 360 of 649 results