We narrowed to 472 results for: Sele

1. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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   Blog Post

   ...requires less biotin, and allows scientists to more selectively target fusion proteins to specific locations ...

2. Antibodies 101: Introduction to Immunofluorescence

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   Blog Post

   ...carefully review your options. If possible, try to select a couple of antibodies that seem promising to compare...

3. New Optogenetic Tools for Cytoskeleton and Membrane Control

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   Blog Post

   ...directed-evolution methods, the activity-based selection in mammalian cells used here could be an attractive...

4. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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   Blog Post

   .... Cre production drives recombination and XFP selection, followed by Cre self-excision. These constructs...

5. Plasmids 101: Common Lab E. coli Strains

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   Blog Post

   ...worked hard designing your plasmid – you carefully selected the optimal promoter for your gene of interest...

6. CRISPR References and Information

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   Collection

   ...This tool can identify positively or negatively selected sgRNAs, genes, or pathways. For use with genome-scale...text file you upload. Currently supports a wide selection of organisms. From the Database Center for Life...sgRNA sequences to maximize on-target activity for selected target(s). Uses human, mouse, and rat reference...PDF, 364 KB Goldstein Nematode: Injection and selection for Cas9-triggered homologous recombination Cas9...Cas9-sgRNA target construct ; pMA122 (negative selection marker); pGH8 (neuronal co-injection marker); ...

7. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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   Blog Post

   ...regulate gene expression) that uniquely express selectable and/or sortable reporters in certain states of...

8. Plasmids 101: Restriction Cloning

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   Blog Post

   ... other materials pass through; and, finally, selectively elute the plasmid DNA from the column using a...

9. CRISPR 101: Epigenetics and Editing the Epigenome

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   Blog Post

   ...Co-expression markers EGFP and PuroR enable sorting and selection of transduced cells. Grant Challen’s lab also ...

10. A Control for All Seasons

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    Blog Post

    ...101: Choosing the Right Isotype Antibodies 101: Selecting the Right Antibody Resources on Addgene.org Addgene's...

11. Building Global Connections with the International Mentorship Program USA-EUROPE

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    Blog Post

    ...suggested important things to emphasize in order to be selected in foreign labs.” Elena Grosso (Mentee IMP 2014...

12. Plasmids 101: Dimers and Multimers

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    Blog Post

    ...although this can be prevented via antibiotic selection (Crozat, 2014). How to Detect Plasmid Multimers...

13. Bacterial Expression Systems

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    Collection

    ...Consortium (SGC) collection page contains an extensive selection of plasmids and tags for protein purification.... and mammalian cells. Addgene Blog A Guide to Selecting Fluorescent Dyes and Ligands GFP Fusion Proteins...Check out our Fluorescent Protein Guide for a selection of mammalian and bacterial FRET-related vectors...Inducible Expression Collection for an extensive selection of plasmids using tetracycline-regulated transcriptional.... Check our Cre-Lox Plasmids Collection for a selection of plasmids containing recombinases to induce ...

14. 9 tips for a successful postdoctoral experience

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    Blog Post

    ...Similarly, there are several factors to consider when selecting a postdoc mentor and lab. These topics have been...

15. Sequencing Options for CRISPR Genotyping

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    Blog Post

    ...worth the cost and labor when single clones are selected. Conversely, translational research may require...

16. Finding Your Science Policy Path

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    Blog Post

    ...your general scientific expertise and then once selected, you can pivot to a completely new field without...

17. Antibodies 101: Validation

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    Blog Post

    ...Still trying to choose an antibody? Check out our Selecting an Antibody blog post! Learn more about Antibodies...

18. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    Blog Post

    ...interest and recombinase-dependent AAV vectors for selective transgene expression. While this is a powerful...

19. Live and Let Dye: Self-Labeling Protein Tags

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    Blog Post

    ...Through Chemistry Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands GCE4All: Making Genetic...

20. CRISPR Plasmids - Tagging

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    Collection

    ...(eg GFP, Flag, YFP, etc) and selection markers. Two independent selection markers are available. An improved... repair) donor plasmid with homology arms and selection marker. The first deposited plasmids in this CRISPR-Cas... the Cas9-programming sgRNA and HR donors for selectable genome modification. This system can be used ...