We narrowed to 466 results for: Sele
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TypeCollection...This tool can identify positively or negatively selected sgRNAs, genes, or pathways. For use with genome-scale...text file you upload. Currently supports a wide selection of organisms. From the Database Center for Life...sgRNA sequences to maximize on-target activity for selected target(s). Uses human, mouse, and rat reference...PDF, 364 KB Goldstein Nematode: Injection and selection for Cas9-triggered homologous recombination Cas9...Cas9-sgRNA target construct ; pMA122 (negative selection marker); pGH8 (neuronal co-injection marker); ...
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Cre-lox system
TypeCollection...removing Stop sequences; Puro selection. See plasmid 35688 for Hygro selection. Mammalian Jacks 11925 pBS302...We also offer ready-to-use viral preparations of select Cre expression plasmids. Addgene's viral service...pBS-OMP-CreFNF OMP targeting vector with Cre FNF (neo-selectable marker flanked by FRT sites) Mammalian Mombaerts...IRES-CreFNF M71 targeting vector with IRES CreFNF (neo-selectable marker flanked by FRT sites) Mammalian Mombaerts...evaluating infection/transfection efficiency, selecting desired clones, and making transgenic mice. ID...Hughes 16422 betaRBPIG Double fluorescent, double selectable cre/loxP reporter Mammalian Hescheler 12463 p231... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post...suggested important things to emphasize in order to be selected in foreign labs.” Elena Grosso (Mentee IMP 2014... -
A Control for All Seasons
TypeBlog Post...101: Choosing the Right Isotype Antibodies 101: Selecting the Right Antibody Resources on Addgene.org Addgene's... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...Co-expression markers EGFP and PuroR enable sorting and selection of transduced cells. Grant Challen’s lab also ... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...although this can be prevented via antibiotic selection (Crozat, 2014). How to Detect Plasmid Multimers... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog Post...regulate gene expression) that uniquely express selectable and/or sortable reporters in certain states of... -
Plasmids 101: Restriction Cloning
TypeBlog Post... other materials pass through; and, finally, selectively elute the plasmid DNA from the column using a... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...worth the cost and labor when single clones are selected. Conversely, translational research may require... -
9 tips for a successful postdoctoral experience
TypeBlog Post...Similarly, there are several factors to consider when selecting a postdoc mentor and lab. These topics have been... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post...Through Chemistry Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands GCE4All: Making Genetic... -
Antibodies 101: Validation
TypeBlog Post...Still trying to choose an antibody? Check out our Selecting an Antibody blog post! Learn more about Antibodies... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...interest and recombinase-dependent AAV vectors for selective transgene expression. While this is a powerful... -
Finding Your Science Policy Path
TypeBlog Post...your general scientific expertise and then once selected, you can pivot to a completely new field without... -
CRISPR Plasmids - Tagging
TypeCollection...(eg GFP, Flag, YFP, etc) and selection markers. Two independent selection markers are available. An improved... repair) donor plasmid with homology arms and selection marker. The first deposited plasmids in this CRISPR-Cas... the Cas9-programming sgRNA and HR donors for selectable genome modification. This system can be used ... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post...is constantly in flux and is dependent on the selective pressures placed upon it by its environment and... -
Typing CRISPR Systems
TypeBlog Post... RNA interference, as it can target and degrade select RNA transcripts, or be engineered to conduct direct... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post... experimental requirements, it’s a good idea to select an available kit that best fits your needs and ... -
Antibodies 101: Flow Cytometry
TypeBlog Post... In this example, the lymphocyte population is selected and analyzed further by plotting CD3 versus CD19... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...cell type you work with (optimization includes selection of transfection buffer and electroporation pulse...