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...Still trying to choose an antibody? Check out our Selecting an Antibody blog post! Learn more about Antibodies...

...interest and recombinase-dependent AAV vectors for selective transgene expression. While this is a powerful...

...your general scientific expertise and then once selected, you can pivot to a completely new field without...

...Similarly, there are several factors to consider when selecting a postdoc mentor and lab. These topics have been...

...worth the cost and labor when single clones are selected. Conversely, translational research may require...

... In this example, the lymphocyte population is selected and analyzed further by plotting CD3 versus CD19...

... RNA interference, as it can target and degrade select RNA transcripts, or be engineered to conduct direct...

...cell type you work with (optimization includes selection of transfection buffer and electroporation pulse...

...is constantly in flux and is dependent on the selective pressures placed upon it by its environment and...

... experimental requirements, it’s a good idea to select an available kit that best fits your needs and ...

...Through Chemistry Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands GCE4All: Making Genetic...

...predicting CGBE editing outcomes and assisting sgRNA selection is available at http://www.sunlab.fun:3838/BE_...

...accumulation of harmful mutations More, due to selection from more cycles of proliferation Less Cell ...

...pay for, but one that you are invited to join (selected due to expertise in field). Publicity in major...

...first, the Liu lab used phage-assisted evolution selection systems to generate ABE8e(TadA-8e V106W), which...

...Experiment Introduction Select Genetic Manipulation Design and Clone gRNA Select Expression and Delivery...expression vectors . Select Your Expression and Delivery System Once you have selected your target and designed... to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering... CRISPR experiment. Created with BioRender.com. Select Your Desired Genetic Manipulation Different genetic...manipulations require different CRISPR components. Selecting the genetic manipulation you plan to perform will... in mind when designing and cloning your gRNAs. Select Genetic Element to be Targeted The exact region...of your protein expressed in your system. HDR : Select a cut site as close as possible to the location...

... plasmid with puromycin selection. See plasmid 24150 for hygromycin selection. Bob Weinberg 10878 pLKO... of shRNA with puromycin selection. See plasmid 21916 for neomycin selection. Dmitri Wiederschain 11578...expression of shRNA with neomycin selection. See plasmid 21915 for puromycin selection. Dmitri Wiederschain 36084...with EGFP marker. See plasmid 14749 for Thy1.1 selection. Christophe Benoist and Diane Mathis 21915 Tet-pLKO-puro...shRNAs or miRNAs, and use a wide variety of drug selection markers. See Campeau et al. (2009) for additional... CMV-driven expression of cDNA with puromycin selection. Ie-Ming Shih 25737 pSLIK-Hygro 3rd Tet-based ... gateway plasmid, and constitutive hygromycin selection. See Shin et al. (2006) for additional variants...

...Promoter Selectable Marker PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter Selectable Marker...Promoter Selectable Marker PI Publication Return to top Nick ID Plasmid Gene/Insert Promoter Selectable Marker...Promoter Selectable Marker PI Publication Return to top RNA Editing ID Plasmid Gene/Insert Selectable Marker...Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR...Mammalian". Activate ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Base Edit...Return to top Epigenetics ID Plasmid Gene/Insert Selectable Marker PI Publication Return to top Interfere...top Prime Edit ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Purify ID...

... you can find or select for only the cells that received the plasmid. Selectable Marker...plasmids with G418 selection Find nematode transgenesis plasmids with Neomycin selection Puromycin Mammalian...A selection of Addgene's empty vector backbones curated by species, epitope tags/fusion proteins, function...function, selectable markers, and more. Plasmid...cloning site (MCS), an inducible promoter, and/or a selectable marker. They are frequently used in molecular...many elements to consider. Here is a guide to a selection of Addgene's empty vector backbones. For the most...Genome Modification, mRNA Regulation, and More Selectable Markers Species-Specific Expression If you want...

...Schwab, M., Suda, K., and Thoenen, H. (1979). Selective retrograde transsynaptic transfer of a protein...