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We narrowed to 562 results for: Spr

Showing: 341 - 360 of 562 results

  1. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ..., they eliminate the expression of the gene (by CRISPR knockout, shRNA depletion, etc.) and ask what the...e67667. DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007...

  5. Cultivating Community Science at BosLab

    Type
    Blog Post
    ..., our journal club discussion on Kevin Esvelt’s CRISPR/Cas9 gene drive paper was very popular. People ...Blog Experimenting in an Open Source Lab: from CRISPR to Cats Stem Cell Models for Disease & Open Science...

  6. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...analysis script should return the same output: a spreadsheet of read counts. You can use these counts to confirm...Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries Resources on Addgene.org Browse Pooled...

  8. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ... of this far-red/red light-responsive system is spread across three plasmids, with the response regulator...limited by a few factors. These bulky systems were spread over a number of plasmids, with one RR (ompR) encoded...

  9. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Representative Empty Backbones CRISPR Genome modification lentiCRISPR v2 - 3rd gen lentiviral vector ... Lentiviral shRNA expression lentiCRISPRv2 hygro - Variant of lentiCRISPR v2 that confers hygromycin resistance...for RNA-guided genome editing in plants Visit our CRISPR Plasmids and Resources collection, and browse our...

  11. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...inducibly depending on the vector and virus. Viral CRISPR-Cas9 tools have been engineered to introduce frameshift...Discuss Gene Therapy with AAV Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells...

  13. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...should be come available in the next few weeks. CRISPRi pooled library The Dolcetto pooled library is now...now available as a lentiviral prep. It's a human CRISPR inhibition library that inhibits over 18,000 human...

  14. AAV Q&A with Tim Miles

    Type
    Blog Post
    .... The continued development of more and smaller CRISPR tools is an exciting area for future AAV application...- a primer for beginners Adenoviral delivery of CRISPR/Cas9 Tips for a first time AAV user Resources ...

  15. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...chemiluminescent proteins for imaging, but it has precluded widespread adoption of the technology. In 2012, Saito et...genes for endogenous protein fusions created using CRISPR/Cas9. eNLs offer high luminescent signal for imaging...

  16. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...Save Time and Track Your Experiments Try out the CRISPR Software Matchmaker Additional Resources on Addgene.org...Protocols for Your Next Experiment Find Our List of CRISPR gRNA Design Tools ...

  17. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...needs to be added to media after autoclaving. If spread on top of pre-made plates, make sure it is evenly...Although blue-white screening is probably the most widespread way to select for plasmids containing an insert...

  18. Plasmid Collections

    Type
    Collection
    ...plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors... Genome Engineering Genome Engineering Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent...pooled libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular set of plasmids...view all collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic...

  19. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...transient gene expression in a particular cell type to CRISPR genome editing, optogenetics, and chemogenetics...different ages." Retinal Degenerative Diseases. Springer International Publishing, 2016. 501-507. PubMed...
Showing: 341 - 360 of 562 results