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  1. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn ...Addgene.org Check out Our Microbiology Resource Page CRISPR plasmids designed for use in bacteria Deposit Your...

  2. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...you’re looking for. Tip 3: Share protocols and spreadsheets with lab members Now that you’ve organized your... may also want to consider moving your paper spreadsheets for reagent storage locations, such as the list...

  3. Distribution to Industry

    Type
    Collection
    ...Mutation Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation...Featured Collections COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson...

  4. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...proteins to monitor the localization of these genes, CRISPR to disrupt their function, and C. elegans overexpression...peritoneal mesothelioma tumorigenesis. They plan to use CRISPR and lentiviral plasmids from Addgene to knockout...

  7. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ..., they eliminate the expression of the gene (by CRISPR knockout, shRNA depletion, etc.) and ask what the...e67667. DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007...

  11. Cultivating Community Science at BosLab

    Type
    Blog Post
    ..., our journal club discussion on Kevin Esvelt’s CRISPR/Cas9 gene drive paper was very popular. People ...Blog Experimenting in an Open Source Lab: from CRISPR to Cats Stem Cell Models for Disease & Open Science...

  12. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...analysis script should return the same output: a spreadsheet of read counts. You can use these counts to confirm...Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries Resources on Addgene.org Browse Pooled...

  14. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ... of this far-red/red light-responsive system is spread across three plasmids, with the response regulator...limited by a few factors. These bulky systems were spread over a number of plasmids, with one RR (ompR) encoded...

  16. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...should be come available in the next few weeks. CRISPRi pooled library The Dolcetto pooled library is now...now available as a lentiviral prep. It's a human CRISPR inhibition library that inhibits over 18,000 human...

  18. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...inducibly depending on the vector and virus. Viral CRISPR-Cas9 tools have been engineered to introduce frameshift...Discuss Gene Therapy with AAV Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells...

  19. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...chemiluminescent proteins for imaging, but it has precluded widespread adoption of the technology. In 2012, Saito et...genes for endogenous protein fusions created using CRISPR/Cas9. eNLs offer high luminescent signal for imaging...

  20. AAV Q&A with Tim Miles

    Type
    Blog Post
    .... The continued development of more and smaller CRISPR tools is an exciting area for future AAV application...- a primer for beginners Adenoviral delivery of CRISPR/Cas9 Tips for a first time AAV user Resources ...
Showing: 341 - 360 of 556 results