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  1. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ..., M., & Al-Qahtani, M. (2018). Evaluation of in vitro chondrocytic differentiation: A stem cell research...Minguela Puras, A., Pons, J. A., & Muro, M. (2018). In vitro intracellular IFNγ, IL-17 and IL-10 producing T ...

  2. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...fusing mutants of Cas9 deficient in DNA cutting activity to a transcriptional activation domain (Fig 1)...Worry less about off targets Unlike Cas9 cutting activity, off target effects are generally not regarded...

  3. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...using microscopy techniques. FRET measurement sensitivity makes it suitable for studying interactions within...used to measure conformational changes, cleavage activity using FRET-based biosensors, and interactions ...

  5. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...indirect labeling is through antibody host species reactivity. Just like for western blotting, a primary epitope-targeting...biotin-streptavidin binding and antibody host species reactivity. When sorting cells with a high resolution for...

  6. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...vector that enables protein expression in any in vitro translation system. The gene set was carefully chosen...been used to produced recombinant proteins for in-vitro assays: Koch, Daniel, et al. "A pull-down procedure...

  9. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...Read on to learn how CRISPR can take on HIV-1 in vitro, and what obstacles must be overcome for clinical...off-target cleavage. In both cases, translating the in vitro work into an animal model comes with two key hurdles...

  10. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...from pLac repressed more   LacZ β-galactosidase activity abolished   lacY Lactose permease inactivated...increases plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability, reduced...

  11. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...promoter upstream of the left homology arm, perform in vitro transcription, and use the IVT product as a template... design has been shown to increase T7 promoter activity. To avoid re-cutting by Cas9 after editing, make...

  12. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for endogenous protein tagging as it is only 19...No photoexcitation High Ion specificity High sensitivity Easy expression Gives ratiometric data The result...

  13. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...genome to be probed for proteins with host-binding activity without the need for genome sequencing or annotation...nitrocellulose for subsequent probing for cell-binding activity.  First, cut out 20 Petri dish-sized circles of...

  14. A Control for All Seasons

    Type
    Blog Post
    ...specificity control, label control, and cross-reactivity control when multiplexing. A, wild-type and knockout... included to uncover secondary antibody cross-reactivity. Non-specific binding  Primary antibody specificity...

  15. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...problems for gene therapy applications. Cross-reactivity of AAV serotypes varies by species and route ...therapeutic outer retinal gene delivery from the vitreous. Science Translational Medicine, 5(189), 189ra76...

  16. Antibodies 101: Validation

    Type
    Blog Post
    ...core goals of antibody validation:   Confirm reactivity with the target antigen - Recognition of an immunogen...methods. These approaches have relatively low sensitivity, which can be problematic if you are trying to...

  17. Finding Your Science Policy Path

    Type
    Blog Post
    ...Plus, just one role can encompass many of these activities leading to a rich day-to-day work life.  Action...professional societies, and industry through convening activities, formal lobbying, or strategy setting.  The “...

  19. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    ...have allowed for real-time monitoring of neuronal activity across substantial 3D volumes. In addition to ...Weijian, and Rafael Yuste. In vivo imaging of neural activity. Nature Methods 14.4 (2017): 349-359. PubMed PMID...
Showing: 341 - 360 of 576 results