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  1. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ... lead to a wealth of additional experiments investigating the role of individual genes or entire pathways...genome-wide screening. Avoid false positives and false negatives – As with any experiment, the use of appropriate...

  3. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...professional. Dr. Cook served as a principal investigator for several research grants. One of the benefits...benefits of being a principal investigator is that you are asked to review grant proposals from others. ...

  5. The Challenges of Cell Culture

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    Blog Post
    ...Virologists too have benefited from the ability to propagate viruses in cell culture; if there were no cells...line consisting of records of the origin and propagation of the line, and can include assays for authentication...

  6. Neuronal labeling with Spaghetti Monster

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    Blog Post
    ...Also, overexpression of FPs can trigger protein aggregation which is toxic for the cells and affects the ...Furthermore, many of these FPs are cytotoxic, prone to aggregation and cross-react with anti-GFP antibodies, making...

  7. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...translation. The ribosome may slow and pause during elongation which may actually be necessary for proper protein...Wobble base-pairing slows in vivo translation elongation in metazoans. RNA 17:2063–2073 . https://doi....

  8. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post
    ...of several popular strains of E. coli for DNA propagation. While great for cloning purposes, these E. coli...hydrophobic regions or multiple disulfide bonds may aggregate and become insoluble. These insoluble globs of...

  9. Tips for Using FRET in Your Experiments

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    Blog Post
    ...used with Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest... is difficult to address especially when you investigate unknown molecular complexes. But it is a parameter...

  10. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...expression mRNA Strong mammalian expression from human elongation factor 1 alpha Constitutive  Tends to give consistent...TEF1 General expression mRNA Yeast transcription elongation factor promoter Constitutive  Analogous to mammalian...

  11. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    Blog Post
    ...has a positive prediction rate of 95% and a 100% negative prediction rate. It is also the fastest test that...University. Shravanti Suresh is a PhD candidate who investigates cellular aspects of CRISPR/Cas immune systems...

  13. Tips for Getting a Faculty Position

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    Blog Post
    ...faculty search committees. Having successfully navigated the trials and tribulations of the process, I’...It helps to come from the lab of a well-known investigator. If you only have middle author papers or no...

  14. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    Blog Post
    ... IDLVs were first discovered by researchers investigating HIV integrase mutants in the early 1990s (Leavitt...of SCID-X1 patients. The Journal of Clinical Investigation, 118(9), 3143–3150. https://doi.org/10.1172/...

  15. Five Popular Model Organisms

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    Blog Post
    ...misfolding that leads to a build-up of protein aggregates termed “lewy bodies” in the brain. Yeast models...other model organisms. Worms have been used to investigate an array of biological systems and processes ...

  18. Adeno-associated Viruses (AAVs) for Genome Editing

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    Blog Post
    ...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency.... "Intact cohesion, anaphase, and chromosome segregation in human cells harboring tumor-derived mutations...

  19. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ... to be replicated. Because E. coli are easily propagated and have a generation time of around 20 minutes...you are working with Arabidopsis check out the GreenGate Toolkit which allows for easy cloning of constructs...

  20. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...financial values. All too often, we see early investigators making unnecessary short term sacrifices that...solutions to reach your goals. For example, a junior investigator turned down a faculty position because none ...
Showing: 341 - 360 of 625 results