We narrowed to 633 results for: gats

...Also, overexpression of FPs can trigger protein aggregation which is toxic for the cells and affects the ...Furthermore, many of these FPs are cytotoxic, prone to aggregation and cross-react with anti-GFP antibodies, making...

...Virologists too have benefited from the ability to propagate viruses in cell culture; if there were no cells...line consisting of records of the origin and propagation of the line, and can include assays for authentication...

...of several popular strains of E. coli for DNA propagation. While great for cloning purposes, these E. coli...hydrophobic regions or multiple disulfide bonds may aggregate and become insoluble. These insoluble globs of...

...used with Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest... is difficult to address especially when you investigate unknown molecular complexes. But it is a parameter...

...expression mRNA Strong mammalian expression from human elongation factor 1 alpha Constitutive  Tends to give consistent...TEF1 General expression mRNA Yeast transcription elongation factor promoter Constitutive  Analogous to mammalian...

... to be replicated. Because E. coli are easily propagated and have a generation time of around 20 minutes...you are working with Arabidopsis check out the GreenGate Toolkit which allows for easy cloning of constructs...

...has a positive prediction rate of 95% and a 100% negative prediction rate. It is also the fastest test that...University. Shravanti Suresh is a PhD candidate who investigates cellular aspects of CRISPR/Cas immune systems...

... IDLVs were first discovered by researchers investigating HIV integrase mutants in the early 1990s (Leavitt...of SCID-X1 patients. The Journal of Clinical Investigation, 118(9), 3143–3150. https://doi.org/10.1172/...

...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency.... "Intact cohesion, anaphase, and chromosome segregation in human cells harboring tumor-derived mutations...

...faculty search committees. Having successfully navigated the trials and tribulations of the process, I’...It helps to come from the lab of a well-known investigator. If you only have middle author papers or no...

...misfolding that leads to a build-up of protein aggregates termed “lewy bodies” in the brain. Yeast models...other model organisms. Worms have been used to investigate an array of biological systems and processes ...

...several ways to measure stickiness. In vitro, ultracentrifugation or gel filtration is the most common analytical...protein of interest usually outweigh potential negative consequences of the tag. Many thanks to our guest...

...introduced using a plasmid construct called Zebrabow-GateDest containing an RFP, CFP, and YFP downstream of ...Mouse Model of Limbal Stem Cell Deficiency." Investigative ophthalmology & visual science 59.3 (2018): ...

...proteins this would lead to protein/organelle aggregation. The Ting lab hypothesized that the low sensitivity...Interactions through Spatial Proximity." Journal of Investigative Dermatology 137.12 (2017): e197-e203. PubMed...

...financial values. All too often, we see early investigators making unnecessary short term sacrifices that...solutions to reach your goals. For example, a junior investigator turned down a faculty position because none ...

...involved in endoplasmic reticulum quality control navigate a large and interconnected system of dynamic and...most prevalent inherited diseases. The authors investigated the role of cytosolic ubiquitin ligases in the...

...population-wide (as opposed to single-cell) approach is to interrogate state with qPCR. The re-arrangement of DNA segments... in each state. The three methods of state interrogation mentioned above (Sanger sequencing, qPCR, and...

...adequate expression is reached you may obtain a false negative or conclude the viral vector is not working.  ...expression from recombinase-dependent AAV vectors and mitigation with cross-over insensitive ATG-out vectors. ...

...postdoc” is, and that “PI” stood for principal investigator. In basic science (as opposed to translational...in a lab. Position Common tasks Principle investigator (PI) Runs the lab Writes a lot of grants Varying...

... developing recombinant antibodies, antibody conjugation, chimeric antibodies, and smaller engineered ...engineered antibodies, including chimerization, conjugation, and antibody fragments. Fab: fragment antigen...