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We narrowed to 633 results for: gats

Showing: 341 - 360 of 633 results
  1. The Challenges of Cell Culture

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    Blog Post
    ...Virologists too have benefited from the ability to propagate viruses in cell culture; if there were no cells...line consisting of records of the origin and propagation of the line, and can include assays for authentication...
  2. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post
    ...of several popular strains of E. coli for DNA propagation. While great for cloning purposes, these E. coli...hydrophobic regions or multiple disulfide bonds may aggregate and become insoluble. These insoluble globs of...
  3. Tips for Using FRET in Your Experiments

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    Blog Post
    ...used with Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest... is difficult to address especially when you investigate unknown molecular complexes. But it is a parameter...
  4. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post
    ...expression mRNA Strong mammalian expression from human elongation factor 1 alpha Constitutive  Tends to give consistent...TEF1 General expression mRNA Yeast transcription elongation factor promoter Constitutive  Analogous to mammalian...
  5. Adeno-associated Viruses (AAVs) for Genome Editing

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    Blog Post
    ...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency.... "Intact cohesion, anaphase, and chromosome segregation in human cells harboring tumor-derived mutations...
  6. Five Popular Model Organisms, Part 2

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    Blog Post
    ... to be replicated. Because E. coli are easily propagated and have a generation time of around 20 minutes...you are working with Arabidopsis check out the GreenGate Toolkit which allows for easy cloning of constructs...
  7. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    Blog Post
    ...has a positive prediction rate of 95% and a 100% negative prediction rate. It is also the fastest test that...University. Shravanti Suresh is a PhD candidate who investigates cellular aspects of CRISPR/Cas immune systems...
  8. Five Popular Model Organisms

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    Blog Post
    ...misfolding that leads to a build-up of protein aggregates termed “lewy bodies” in the brain. Yeast models...other model organisms. Worms have been used to investigate an array of biological systems and processes ...
  9. Tips for Getting a Faculty Position

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    Blog Post
    ...faculty search committees. Having successfully navigated the trials and tribulations of the process, I’...It helps to come from the lab of a well-known investigator. If you only have middle author papers or no...
  10. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    Blog Post
    ... IDLVs were first discovered by researchers investigating HIV integrase mutants in the early 1990s (Leavitt...of SCID-X1 patients. The Journal of Clinical Investigation, 118(9), 3143–3150. https://doi.org/10.1172/...
  11. How to Negotiate a Successful Lab Start Up Budget

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    Blog Post
    ...financial values. All too often, we see early investigators making unnecessary short term sacrifices that...solutions to reach your goals. For example, a junior investigator turned down a faculty position because none ...
  12. Exploring Applications of the Bioluminescent HiBiT Tag

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    Blog Post
    ...involved in endoplasmic reticulum quality control navigate a large and interconnected system of dynamic and...most prevalent inherited diseases. The authors investigated the role of cytosolic ubiquitin ligases in the...
  13. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...establish your own lab and get an independent investigator grant (e.g. an R01 from the NIH, in the United...can be found during conversations with senior investigators. Discussions can reveal mutual scientific interests...
  14. Transferable Skills Guide: Teamwork

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    Blog Post
    ...talent, of human knowledge, that has ever been gathered at the White House - with the possible exception...someone to coordinate, someone to decide, someone to delegate, etc. If you find that there is not someone (or...
  15. Important Considerations When Using AAVs

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    Blog Post
    ...Drugs, DREADDs) and optogenetics as tools to investigate the roles of certain cell types in locomotion...cause the least amount of immune response. Any investigator who is using an in vivo animal model should ...
Showing: 341 - 360 of 633 results