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Showing: 341 - 360 of 878 results

  1. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...research? Check out Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0...1.0, 1.1, 2.1 plasmids Brainbow 3.0, 3.1, 3.2 plasmids References: Transgenic strategies for combinatorial... of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick primer on how...

  2. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    ... labs have deposited plasmids to Addgene and we distribute over 130,000 plasmids in 2014. Thus, we are...trends for Zinc Finger, TALE, CRISPR, and SynBio plasmids over time. You can see these data in the slide...molecular biology technology? Have you deposited your plasmids yet?      ...

  3. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... Researchers have been sharing plasmids ever since there were plasmids to share. Back when I was in the...specific strategies and tactics for getting the plasmids he wanted. Addgene makes it so much easier to ...CyOFP1-NLuc-CyOFP1; Antares). Direct injection of plasmids for Antares or firefly luciferase into mouse livers...Additional Resources on Addgene.org Browse Luciferase Plasmids Browse the Fluorescent Protein Collection Find...

  4. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...thumb, we avoid target sites that code for amino acids near the N’ terminus of the protein, in order to...Likewise, we avoid target sites that code for amino acids close to the C’ terminus of the protein, to maximize...dCas9-mediated transcriptional repression. Nucleic Acids Res 44:e141–e141 . https://doi.org/10.1093/nar/gkw583...scarless human stem cell genome editing. Nucleic Acids Research 41:9049–9061 . https://doi.org/10.1093/...CRISPR Basics with Our CRISPR Guide Find CRISPR Plasmids for Your Research Find CRISPR Pooled Libraries...

  6. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...repository, and explaining how to deposit and request plasmids, but I also spend a lot of time listening. I’ve...scientists (and our growing collection of 25,000+ plasmids from 1,600+ labs around the world!) that have ...inside scoop from scientists who have deposited plasmids at Addgene.  Learn how discoveries were made and... and get practical tips on how to use the plasmids in your own lab. Summaries of the latest technologies...

  7. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    ...quickly. There are over 2,300 COVID-19 related plasmids currently in Addgene’s repository which have been...plasmid deposits came in this year, there were some plasmids deposited as early as 2006 that have helped scientists... of the Addgene community. Perhaps you’ll find plasmids and new tools that will speed your science along...lab closures, we’re expecting to reach ~14,000 plasmids deposited this year once they all get processed...

  8. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    ...Christopher E. French. Dr. Duedu was recently awarded plasmids from Addgene as a successful applicant to the ... fellow nonprofit, Seeding Labs, to distribute plasmids to researchers in developing regions and thereby...plans to use his research experiences as well as plasmids from Addgene to bring new opportunities to Ghanaian...out our Microbiology Pages Find Synthetc Biology Plasmids Find Molecular Biology Resources ...

  9. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...phage pL promoter.  It is a good idea to choose plasmids that also express the repressors associated with...needed. This approach is best suited for modifying plasmids or BACs because it results in stable integration...restriction enzyme digest can be used to screen plasmids for the appropriate mutations.  Point mutations... Homology Directed Repair Use Knockout/Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR Methods...Browse our Microbiology Pages  Find Lambda Red Plasmids and Strains Check Out Other Molecular Biology ...

  10. Reflections and Looking Ahead

    Type
    Blog Post
    ...accelerating scientific discovery. Over 150,000 plasmids empowering researchers worldwide. Countless breakthroughs...countries enriched our repository with 15,020 new plasmids — a testament to the scientific community's commitment...toward giving you more choice in how you receive plasmids — as DNA or bacterial stabs — ‌and reinventing...sequences and characterization data for over 150,000 plasmids, empowering bioinformaticians, data scientists...

  11. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    ...collection of popular plasmid sequences. These plasmids contain features such as antibiotic resistance... boundaries for control elements in commercial plasmids were inconsistent and sometimes clearly wrong....work to fill any common annotation gaps in the plasmids available through the Addgene website. In addition...plasmid analysis powered by SnapGene Browse All Plasmids ...

  12. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...constructs from Christopher Harvey’s lab. These plasmids localize opsins to the soma to avoid off target...neighboring neurons. We have also packaged hChR2(H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn... has now expanded to offer many of our control plasmids in new serotypes. See our Serotype Testing AAV... jGCaMP7 from Janelia, including three jGCaMP7 plasmids now packaged in AAVrg. jGCaMP7s, the variant with...

  13. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...), 339-350 (2015). PubMed. Find the Plasmids @Addgene: Plasmids for CRISPRi are described here: http...http://www.addgene.org/crispr/qi/ Plasmids described in  Zalatan et al. Cell  (2015) can be found here...https://www.addgene.org/browse/article/9593/ Plasmids described in Qi et al. Cell  (2013) can be found...

  14. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...ll work with you to get the word out about your plasmids and their potential educational uses. We believe...30083609. PubMed Central PMCID: PMC6070313. Find the plasmids from this publication at Addgene! Additional ...developmental biology Use the pathways over time plasmids to engage students in functional genomics research...out our molecular biology reference pages Find plasmids from the BioBits BrightTM publication here ...

  15. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...19213877 PMCID: PMC2746483 Find ribosome profiling plasmids at Addgene. 2. Halstead, James M., et al. “Translation...1367-71. PMID: 25792328 PMCID: PMC4451088 Find plasmids from this paper at Addgene. 3. Yan, Xiaowei, ...976-89. PMID: 27153498 PMCID: PMC4889334 Find plasmids from this paper at Addgene. Additional Resources...Resources on Addgene.org Find Fluorescent Protein Plasmids Check Out Other Hot Plasmid Technologies ...

  16. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids for Bacterial Expression ...produced R-bodies that could extend at different pHs. Plasmids expressing expressing both wild type and mutant...acssynbio.5b00237. PubMed PMID: 26814170. Find Plasmids from This Publication at Addgene 4. Petroni, ...

  17. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...just your desired genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric fluorescent...embryonic stem cells.  Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter...Zhao T-T, Connelly JP, Pruett-Miller SM, Blanc V, Davidson NO, Madison BB (2017) A stable but reversible ...Addgene.org Read our CRISPR guide Find CRISPR plasmids for your research Find resources and software ...

  18. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...different plasmids. Harvard’s DRSC/TRiP Functional Genomics Resource Center has various plasmids for overexpression...filtering by fly as species of gene. Find Gal4 and UAS plasmids at Addgene Choosing a vector to generate a transgenic...Recombination Resources at Addgene.org Browse Drosophila Plasmids ...

  19. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...Resources on Addgene.org Browse Cancer Plasmids Browse Immunology Plasmids Find Kits for Cancer Research  ...immunosurveillance have a look at our Immunology Research Plasmids and Resources. Studying the immune system and ...analyse the tumor microenvironment (one of the plasmids on her poster is pTomo-Ras which has been deposited...

  20. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...: 1262. PMID: 23232392 PMCID: PMC3535334 Find plasmids from this paper at Addgene. Takai, Akira, et ...4352-6. PMID: 25831507. PMCID: PMC4394297 Find plasmids from this paper at Addgene. Suzuki, Kazushi, ... 13718. PMID: 27966527 PMCID: PMC5171807 Find plasmids from this paper at Addgene. Additional Resources...All Our Fluorescent Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein ...
Showing: 341 - 360 of 878 results