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We narrowed to 876 results for: lic

Showing: 341 - 360 of 876 results

  1. Tags and Other Markers

    Type
    Collection
    ...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control by Addgene...Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other antibodies...

  2. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...adapted from Andrews et al., 2019 under CC-BY 4.0 license.   Selecting your antibodies When selecting...primary antibodies that have been validated in IF applications (find IHC validated or ICC validated antibodies...978-1-4939-8935-5_26 Jambor, H., Antonietti, A., Alicea, B., Audisio, T. L., Auer, S., Bhardwaj, V., Burgess...informative image-based figures for scientific publications. PLOS Biology, 19(3), e3001161. https://doi....Immunocytochemistry Protocols for other antibody applications...

  3. Neuroscience

    Type
    Collection
    ...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control by Addgene...Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other antibodies...

  4. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    ...within that article you’ll find new plasmids. Simply click on the badge to be taken directly to the plasmids...account on PubMed. Once you have an account, simply click on your account name in the top right corner of ...PubMed to navigate to your account settings. Next, click on the link in the “NCBI Site Preferences” box. ...Preferences page, navigate to “PubMed Preferences” and click on the “Filters & Icons” link (see image to the ...

  6. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ... wanted to inactivate the gene BRAF (a kinase implicated in several human cancers) in A549 cells (a human... Cas9 expressing lentivirus at the indicated multiplicity of infection (MOI). Anti-vinculin Western blotting...need to use FACS (like they did in the original publication). I thought I could see GFP expression with a...expression, but I didn’t. 2. In the original publication they see GFP expression in about 20% of their...an MOI of 0.3, as recommended by the original publication. Pro-Tip: 1. Use multiple gRNAs to target different...

  7. CRISPR Plasmids - Epigenetics

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    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection...

  8. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...immunoprecipitation applications, but none of the traditional tags were suitable for both applications. To prove...These tags are easy to use in multiple common applications. Due to their limited effects on native protein...particularly in single-molecule or high-throughput applications. Perhaps IT5 and IT6 will even replace our older...

  9. Fluorescent Protein Guide

    Type
    Collection
    ...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... Use this guide to learn more about the many applications of fluorescent proteins and to find plasmids...

  10. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled... changes. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression...

  11. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...including that it is easy to implement due to the simplicity of gRNA design, programmable for different genomic...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a ...

  12. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled... Plasmid Gene/Insert Vector Type Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression...

  13. CRISPR Plasmids - Parasites

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled... ID Plasmid Gene/Insert Selectable Marker PI Publication Libraries Toxoplasma CRISPR Knockout Pooled Library...

  14. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot... Sattarzadeh et al., Sci Rep. 2015 CC BY 4.0 Applications and limitations of GFP photoconversion One concern...2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM...nmeth.1664. PubMed. Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein...

  15. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ... QC/QA team as a DNA Sequencing Intern. The application process When I first saw the comprehensive job...immediately clear that Addgene wanted to ensure applicants understood what the expectations were for the...the intended position. After submitting my application, I was contacted to participate in a brief fifteen...the depositing labs intended. With the use of applications like SnapGene and Geneious, I was able to determine...

  16. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ...funding and volunteer opportunities, protocols, publications, and more to accelerate the pace of research...is based in Canada, much of the information is applicable to researchers worldwide. “COVID-19 has spread...advance COVID-19 research to hopefully help guide policies and ultimately treatments in a timely fashion.... a significant impact on both clinical care and public health for this current COVID-19 pandemic, and ...

  17. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...modified the GCN4 sequence to increase its alpha-helical structure and stability, creating v4 SunTag. Since...aggregation, it’s recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al...flames,” it’s clear that the system is broadly applicable across many areas of research. Beyond fluorescence...activation of target genes, but we’ll save that application for another day! References 1. Tanenbaum, Marvin...

  18. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...simply writing down your data doesn’t guarantee a publication. There is an art to composing manuscripts and...field of microbiology with multiple high-profile publications. My classmates consisted of graduate students...well as being critiqued added an extra level of application not present in the online class.   Four ways ...paper will be generated. After you complete a publication quality figure, produce the figure, its legend...

  19. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...experiments The Tian lab used dLight1 both in brain slices and freely behaving mice to gather the following...and concentration of released DA in mouse brain slices Visualization of DA release dynamics during locomotion...GRABDA in drosophila, zebrafish, and mouse brain slices and freely moving mice to gather the following ...from a single dopaminergic fiber in mouse brain slices Demonstrate DA detection in a single neuron in ...

  20. A Tour of Addgene's Most Popular Pooled Libraries

    Type
    Blog Post
    ...them useful for lineage tracing, among other applications. Cells can be identified using fluorescence,...Created with BioRender.com. The popular (and deliciously named) Brie and Brunello libraries have been ...sgRNAs to guide Cas9 to targeted genes. In these applications, however, nuclease-dead Cas9 is fused to a transcriptional...library of all human transcription factors, including splice isoforms, to allow comprehensive screening, cellular...
Showing: 341 - 360 of 876 results