We narrowed to 560 results for: mme
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TypeBlog Post...different role? I think the secret sauce can be summed up in one word – initiative. Initiative is the...
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Management for Scientists: What Makes a Good Manager Anyway?
TypeBlog Post...knowledge-intensive workers”. I have added a few comments of my own to this list. Need feedback on their... -
Choosing Your Perfect Empty Backbone
TypeBlog Post...backbone. Or just ask – leave a message in the comments below and an Addgenie will help you find what ... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog Post...ingenuity to solve this problem. None of the commercial boxes were small enough to minimize the required... -
Antibodies 101: Epitope Tags
TypeBlog Post...from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol. (2003) 60... -
Antibodies 101: Chimeric Antibodies
TypeBlog Post...anti-Protein B antibody (Fig. 3) Figure 3: Recommended secondary antibodies for rat IgG1 and mouse 1gG2a... -
Plasmids 101: Origin of Replication
TypeBlog Post...something clarified? If so please, post in the comments. References Chatterjee A, Sun SB, Furman JL, ... -
5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog Post...that you've been working on, let us know in the comments below! Additional resources on the Addgene blog... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog Post... (pAAV.hSyn-FLEX.iGABASnFR.F102G) is noticeably dimmer than iGABASnFR, and accumulates in the endoplasmic... -
Tetbow: Bright Multicolor Labeling for Neuronal Tracing
TypeBlog Post...large brain samples. For large brain samples, we recommend pretreatment with ScaleCUBIC1 (Susaki et al., ... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post...Limitations with GFP in imaging While GFP has been an immensely powerful tool to study proteins in vivo, it does... -
The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog Post..., office of knowledge transfer, office of commercialization Additional resources on Addgene.org Addgene... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog Post... and named the protein mTFP1. The researchers recommend using mTFP1 with a 445/30 nm excitation filter... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog Post...model system is a mouse, given the wealth of commercially available mouse monoclonal antibodies. The good... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog Post... in ordering from ABRC for the first time, we recommend reaching out to us at [email protected] for help... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog Post...mouse (NEB, E8032S) antibodies against MBP are commercially available. Factor Xa loves calcium, but metal-chelate... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post... annotated boundaries for control elements in commercial plasmids were inconsistent and sometimes clearly... -
The Strength of Story Telling
TypeBlog Post...relationships are all things almost all of us are programmed to appreciate and understand. We build narratives... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...using NgAgo? Let us know how it’s working in the comments section! Read About NgAgo User's First Impressions... -
Plasmid Cloning by PCR
TypeBlog Post...piece (see NEB for more information). Thus, we recommend that you add 3-6 bases upstream of your restriction...