...application of long or short dsRNA. However, it is important to note that naked and unmodified dsRNA elicits... Together, this excellent work highlights the importance of using orthogonal methods to confidently conclude...genome, whereas shRNAs are exogenously derived. Importantly, instead of the assortment of siRNAs generated...

...hybridoma-derived monoclonal antibodies. It is also important to seek out supportive data on candidate antibodies...and/or intracellular labeling (or both). It is important to test for the protocol you intend to use in ...Although the expected location of the target is an important factor in deciding which protocol is likely to...

... temperature should be 60-65°C For qPCR, the important primer variable is the annealing temperature, ...help you in your execution:  Accuracy is not as important as precision for relative quantification. A consistent...better choice (Tichopa, 2003. Marino, 2003) - an important note, as the other most commonly used method is...

...transposition. INTEGRATE consists of four major components including (1) a CRISPR RNA, (2) four proteins... a mammalian cell line (Qi et al., 2013). Two important features of this early system are its regulatable...transcription factors, phage proteins, and RNAP components (Dong et al., 2018). They found a transcription...

...more commonly known as fruit flies, have been an important animal model for research, particularly genetics...stay there. Yet capturing the flies is nearly impossible, due to their small size and inconspicuous coloring...

...protocol and protocol video!  Antibodies The most important part of your western is your antibodies, particularly... weight proteins Long running time   It’s important to note that Bis and Tris gels run proteins in...and better linearity within detection limits (important if you want to quantify your blots). Fluorescent...

...story of Rosalind Franklin and her incredibly important role in solving the structure of DNA. 2. The...popular videos. Describes fossil rocks and their importance in understanding biological history. 4. Please...

...Seeding Labs, Addgene also feels it’s incredibly important for scientific breakthroughs, reagents, and know-how...continues to face global challenges, we hope to empower those that need to face these challenges locally...

...Realizing that Addgene would be successful and the importance of lentiviral vectors in ensuring that success... Addgene's Future, the research team, and the importance of sharing information/knowledge Outro Many ...

...neutralization or targeted destruction.  Antibodies are composed of two light chains and two heavy chains. The ...repositories, such as Addgene and the DSHB, play an important role in scientific exchange. In addition to ensuring...

...identify what your plasmid contains and other important information. Tip: A lowercase "p" is often used...simple piece of information can often convey many important details. Once you know the backbone a plasmid ...

...: Topic 0:00 - 0:50 : Intro 0:51 - 3:08 : The importance of developing a strong graduate student community...you changed throughout graduate school? - The importance of criticism and feedback 15:38 - 17:06 : Post...

...proteins to migrate…so don’t try eliminating one component entirely. If you are trying to transfer proteins...increases (Figure 3A).  This step is especially important for highly expressed proteins, including “housekeeping...don’t want to skimp on your washing steps! It’s important to let them wash for the full amount of time, ...

...ready-to-use recombinant antibodies collection, currently composed of over 100 antibodies. Everything in this collection...validation data, sharing what works and, equally importantly, what doesn’t. Reports receive a DOI for citation...

...mutations, both in mice and in human cells. But that important advance was quickly followed by even splashier...All this, and scientists are still working out important details in their understanding of how CRISPR/Cas9s...

...least one site. Phosphorylation is particularly important for regulating signal transduction and measuring...the nucleus and the cytoplasm, nuclear export and import rates can influence KTR translocation. Work from...

...mammalian cell lines. Cas9, of course, is the key component of most CRISPR systems and generates double strange...are some additional features. The first and most imporant is that, upstream of the Cbh promoter, this plasmid...

...water of HR work. What do you think is the most important resource that Addgene provides the scientific ... how to operate during the pandemic, but it’s important to develop a plan for moving forward. Sooner or...

...containing these base editors Spatially and temporally regulated AAV-based genome editing In 2016, Jonathan...AAV-based genome editing system is regulated not only temporally with doxycycline, but it can also be regulated...

... genetic circuits, allowing for tighter spatio-temporal control of gene expression within a cell. Thanks..., designer PUF proteins can be fused to other components to lend them many other functions for use in ...