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We narrowed to 760 results for: mpo

Showing: 341 - 360 of 760 results

  1. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...application of long or short dsRNA. However, it is important to note that naked and unmodified dsRNA elicits... Together, this excellent work highlights the importance of using orthogonal methods to confidently conclude...genome, whereas shRNAs are exogenously derived. Importantly, instead of the assortment of siRNAs generated...

  2. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...editing technology led to the development of many important medical and agricultural products. But it also...instead of recognizing nucleotide triplets, the components recognize individual nucleotides. This imparts...SciFi is one of the most imaginative and most important genres of literary fiction. It has always served...

  3. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ... phenomenon? Exploration can be important, but it is equally important to have a plan to get to mechanism...outcome, e.g. where could you publish this and how important is the result? Is there a tractable question ...

  4. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ... the University of Cambridge. One of the most important steps in the CRISPR experimental process is validating...silico prediction. These techniques differ in important ways, but can also complement one another by providing...tissue from whole animal models, no exogenous component required (e.g. dsODN), doesn't require high read...

  5. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...hybridoma-derived monoclonal antibodies. It is also important to seek out supportive data on candidate antibodies...and/or intracellular labeling (or both). It is important to test for the protocol you intend to use in ...Although the expected location of the target is an important factor in deciding which protocol is likely to...

  6. Deep Dive: qPCR

    Type
    Blog Post
    ... temperature should be 60-65°C For qPCR, the important primer variable is the annealing temperature, ...help you in your execution:  Accuracy is not as important as precision for relative quantification. A consistent...better choice (Tichopa, 2003. Marino, 2003) - an important note, as the other most commonly used method is...

  8. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...more commonly known as fruit flies, have been an important animal model for research, particularly genetics...stay there. Yet capturing the flies is nearly impossible, due to their small size and inconspicuous coloring...

  9. Technical Design of a Western Blot

    Type
    Blog Post
    ...protocol and protocol video!  Antibodies The most important part of your western is your antibodies, particularly... weight proteins Long running time   It’s important to note that Bis and Tris gels run proteins in...and better linearity within detection limits (important if you want to quantify your blots). Fluorescent...

  10. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    ...story of Rosalind Franklin and her incredibly important role in solving the structure of DNA. 2. The...popular videos. Describes fossil rocks and their importance in understanding biological history. 4. Please...

  11. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...Realizing that Addgene would be successful and the importance of lentiviral vectors in ensuring that success... Addgene's Future, the research team, and the importance of sharing information/knowledge Outro Many ...

  14. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ...identify what your plasmid contains and other important information. Tip: A lowercase "p" is often used...simple piece of information can often convey many important details. Once you know the backbone a plasmid ...

  15. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...: Topic 0:00 - 0:50 : Intro 0:51 - 3:08 : The importance of developing a strong graduate student community...you changed throughout graduate school? - The importance of criticism and feedback 15:38 - 17:06 : Post...

  16. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...proteins to migrate…so don’t try eliminating one component entirely. If you are trying to transfer proteins...increases (Figure 3A).  This step is especially important for highly expressed proteins, including “housekeeping...don’t want to skimp on your washing steps! It’s important to let them wash for the full amount of time, ...

  17. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...ready-to-use recombinant antibodies collection, currently composed of over 100 antibodies. Everything in this collection...validation data, sharing what works and, equally importantly, what doesn’t. Reports receive a DOI for citation...

  18. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...mutations, both in mice and in human cells. But that important advance was quickly followed by even splashier...All this, and scientists are still working out important details in their understanding of how CRISPR/Cas9s...

  20. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...mammalian cell lines. Cas9, of course, is the key component of most CRISPR systems and generates double strange...are some additional features. The first and most imporant is that, upstream of the Cbh promoter, this plasmid...
Showing: 341 - 360 of 760 results