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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 563 results for: pac

Showing: 341 - 360 of 563 results

  1. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... a good story (1). From a deep sea shrimp to a compact powerhouse of bioluminescence, NLuc is 100-fold...LumiFluor reporter constructs were designed to be packaged into a retrovirus for infection of host cells....

  2. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...thus frameshifts that disrupt the protein-coding capacity of a locus. When using S. pyogenes Cas9 (SpCas9.../doi.org/10.1038/s41587-020-0561-9 Dempster JM, Pacini C, Pantel S, Behan FM, Green T, Krill-Burger J,...

  3. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...modifications to DNA or histones, the proteins that help package DNA, were associated with gene expression or silencing...particularly large protein, and this fusion allows for packaging into viral vectors and targeted delivery into ...

  4. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...needed to replicate inside a host cell but with space to insert your genetic cargo of choice. After hearing...taken them for a test drive! We validated viral packaging and strong expression after inserting EGFP and...

  5. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...sample migrate through the stacking gel at the same pace and enter the resolving gel at the same time where...popular due to its durability and high binding capacity. In addition, PVDF membranes can be repeatedly...

  7. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...Furthermore, piggyBac™ offers a large cargo-carrying capacity (over 200 kb has been demonstrated) with no known...certain genes, such as those that have negative impacts on fitness. One consideration when making a transgenic...

  10. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ... epigenetic modification, bulges) that may also impact off-target edits. As a result, only sequencing ...algorithms may not provide a full picture of the impact of CRISPR editing in the model cell line or organism...

  11. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...populations that are the most likely to be positively impacted by the trial. During clinical trials, expansion... strategies that synergize with these positive impacts on the immune system will likely have better treatment...

  12. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...distance in the placement of plasmid elements can impact expression levels and consequently convolute the... different lentiviral plasmid backbone elements impact viral titer and to compare different Cas enzymes...

  15. Deep Dive: qPCR

    Type
    Blog Post
    ...multiplex your reference genes to save on plate space. For many cell lines and tissue types, appropriate...your reference gene set can save significant plate space and may be worth the initial effort of setting it...

  16. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...combination of all 11 att pairs in series with unique spacer sequences (pMemoryArray) gives a DNA sequence capable...compartment (e.g. the plasma membrane, the extracellular space, or the lysosomes) can take many paths. Understanding... to other viral systems due to its large cargo capacity, minimal cytotoxicity, and broad host-tropism....

  17. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... Gateway-Compatible Toolbox Podcast Segment The impact of KLF4 N-terminal variants on iPSC generation ... expression levels; this altered stoichiometry impacted reprogramming and global gene expression patterns...

  18. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...each DNA target have high sequence identity to the spacer sequence and contain a PAM sequence just downstream...

  20. Resolute Plasmid Collection

    Type
    Collection
    ...research output, RESOLUTE expects to accelerate the pace of research in the field of SLCs to the global benefit...
Showing: 341 - 360 of 563 results