...new window) Link to Video Making LB Agar Plates Create plates to culture bacteria in the lab Watch the...Video! Over-Agar Antibiotic Plating Quickly add antibiotic to a pre-poured plate Watch the Video! Streaking...Bacteria Isolate single bacterial colonies on an agar plate Watch the Video! Inoculating a Liquid Bacterial ...

...with shaking platform set to 120 rpm 37 °C bead bath Vortex Stir bar Magnetic stir plate pH meter Reagents...water in a 1 L bottle and stir on a magnetic stir plate. Stir until all particles have dissolved. Note: ...flask.Incubate in a 37 °C, 5% CO 2 incubator on a shaking platform set to 120 rpm. Pro-Tip Do not use cells that ...

...days. Single cells were then plated in individual wells of a 96-well plate and left undisturbed for 13 ... well (each well gets one dilution, so a 6-well plate will hold 5 dilutions plus one 'no virus' control...

...competent cells and retransform. Bacteria on an LB agar plate can be stored at 4°C for a few weeks. However, if...stock unthaw! Streak the bacteria onto an LB agar plate . Grow your bacteria overnight at the appropriate...

...(μL) Volume of Virus Added to Plate (μL) Volume of Cells Added to Plate (μL) Final Viral Dilution 1:10...

...controller Hazardous waste container pH meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex...reach 100% confluence (80–90% is ideal). Pass and plate the cells the day before the transfection. Thaw ...

..., it is a good idea to place the gel on a glass plate if available. Unlike the plastic tray, this will...

...2-3μL into your favorite competent bacteria and plate. Be sure to pick multiple colonies for mini-prepping...

...sequence is used, plus a minimum of 18 bp of your template sequence. 5' and 3' primers will have different...

...liquid into different tubes or into wells of a microplate, you may consider using a multichannel pipette...

...bacterial culture. For details on how to streak a plate to get individual colonies and to generate liquid...

...spectrophotometer 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead bath Clamp stand and...