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We narrowed to 792 results for: post

Showing: 341 - 360 of 792 results

  1. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...use restriction enzymes or ligase, and requires no post-PCR procedures. Sounds easy right? The technique...thymine (T) to hybridize and form hydrogen bonds. This post focuses on "sticky end" TOPO (also called TOPO-TA...

  2. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...This post was contributed by guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’... detailed investigations into the release of DNA post-cut. Using substrate DNA labeled on both sides of... optional donor DNA. Chris Richardson is a Postdoctoral Researcher in Jacob Corn’s lab investigating...

  4. 2022 Depositor Week

    Type
    Blog Post
    ... our Depositor Week schedule:  Monday: Thank you post (this is it!)  Tuesday: Stats roundup on Twitter...

  6. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...again, this post has a lot of acronyms, so we've included a glossary at the end of this post! Figure...In our last post, we talked about the first base transversion editors: CGBEs, or C → G Base Editors. ...

  7. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...right time. Like many of the posts in our Transferable Skills series, this post is not meant to be a comprehensive...details and vocabulary for this skill. What this post may do is help you realize whether or not you have... a conference poster session, you don’t necessarily want everyone who visits your poster to agree with...

  8. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of ...obstruct binding sites or motifs that are necessary for post-translational modifications. Despite these potential...Addgene blog Read our fluorescent proteins blog posts Download the fluorescent proteins 101 eBook Read...

  9. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...array of different techniques and protocols. In this post we will discuss the natural role of biotin, biotinylation...ligase (BPL) is responsible for this highly specific post-translational protein modification. Most organisms.... E. “The enzymatic biotinylation of proteins: A post-translational modification of exceptional specificity...

  10. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of .... This is clearly the case when proteins undergo post-translational modification with fatty acyl chains...Introduction to GFP Read our Fluorescent Proteins Blog Posts Download the Fluorescent Proteins 101 eBook Resources...

  11. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ... covered five model organisms in a previous blog post, but here are five more model organisms that have... scientific community. (And, check out this blog post to see even more genetic systems for emerging model...organisms in the first Five Popular Model Organisms blog post Read more about plasmids in our Plasmids 101 blog...

  12. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...cloning we once relied exclusively on. In this blog post, I will go over some advantages, disadvantages, ...Independent Cloning (SLIC) in a previous Plasmids 101 post. Although SLIC may be more cost effective, Gibson...the Addgene Blog Browse All of Our Plasmids 101 Posts Read About Other Cloning Techniques Additional ...

  13. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ... smear across your FSH vs reporter plot. In this post, I’ll walk you through how to interpret the antibody...  Note that for the flow plots discussed in this post, voltage has been adjusted appropriately and compensation...

  14. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...hundred trillion connections (synapses.)  In this post we’ll describe the AAV retrograde serotype (AAVrg...new tool, go forth and trace some neurons!  This post was originally written by Leila Haery in 2017 and...

  16. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...This post was contributed by Ignacio Amigo and María Hernández Sánchez from Crowdfight. In March 2020... resources on Addgene's blog Check out our blog post about remote collaborations Read our Transferable...science. Others are experienced researchers, such as postdocs and principal investigators. They also come from...IgnacioAmigoH     María Hernández-Sánchez is a postdoctoral researcher at Instituto de Investigación Biomédica...

  17. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...to improve processes in the Addgene lab. In this post, I’ll tell you a little bit about what I learned... got to summarize my experience in my first blog post! I highly recommend doing the same if you ever go...

  18. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...This post was contributed by Mary Tamer from BioBuilder, an innovative nonprofit bringing the study of...Optogentics for E. coli Browse All Synthetic Biology Blog Posts Resources on Addgene.org Guide to SynBio Assembly...chapter and an illustrated, downloadable classroom poster — with both of these resources road tested over...

  19. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    .... To dive deeper into this field read our recent post “Cancer and the Immune System: Deciphering the Relationship...on the Addgene Blog Read Additional Cancer Blog Posts Addgene's Cancer Collection Pages Cancer and the...open-source! Impressions from the poster sessions During the poster session I got a chance to talk with...tumor microenvironment (one of the plasmids on her poster is pTomo-Ras which has been deposited at Addgene...
Showing: 341 - 360 of 792 results