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TypeBlog Post...Resources Find more career posts on the Addgene blog Read our recent post on Synbio start up Revbio ...
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Lentiviral Vector Guide
TypeGuide... sequence. WPRE in cis Woodchuck hepatitis virus post‐transcriptional regulatory element; stimulates the... -
2022 Depositor Week
TypeBlog Post... our Depositor Week schedule: Monday: Thank you post (this is it!) Tuesday: Stats roundup on Twitter... -
Introducing the Addgene Antibodies 101 eBook!
TypeBlog Post...Each chapter brings together all of our 101 blog posts on an antibody-specific topic, allowing you to easily... -
CRISPR 101: Any Base Transversion Editors
TypeBlog Post...again, this post has a lot of acronyms, so we've included a glossary at the end of this post! Figure...In our last post, we talked about the first base transversion editors: CGBEs, or C → G Base Editors. ... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post...right time. Like many of the posts in our Transferable Skills series, this post is not meant to be a comprehensive...details and vocabulary for this skill. What this post may do is help you realize whether or not you have... a conference poster session, you don’t necessarily want everyone who visits your poster to agree with... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post...This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of ...obstruct binding sites or motifs that are necessary for post-translational modifications. Despite these potential...Addgene blog Read our fluorescent proteins blog posts Download the fluorescent proteins 101 eBook Read... -
Plasmids 101: Biotinylation
TypeBlog Post...array of different techniques and protocols. In this post we will discuss the natural role of biotin, biotinylation...ligase (BPL) is responsible for this highly specific post-translational protein modification. Most organisms.... E. “The enzymatic biotinylation of proteins: A post-translational modification of exceptional specificity... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of .... This is clearly the case when proteins undergo post-translational modification with fatty acyl chains...Introduction to GFP Read our Fluorescent Proteins Blog Posts Download the Fluorescent Proteins 101 eBook Resources... -
Five Popular Model Organisms, Part 2
TypeBlog Post... covered five model organisms in a previous blog post, but here are five more model organisms that have... scientific community. (And, check out this blog post to see even more genetic systems for emerging model...organisms in the first Five Popular Model Organisms blog post Read more about plasmids in our Plasmids 101 blog... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post...cloning we once relied exclusively on. In this blog post, I will go over some advantages, disadvantages, ...Independent Cloning (SLIC) in a previous Plasmids 101 post. Although SLIC may be more cost effective, Gibson...the Addgene Blog Browse All of Our Plasmids 101 Posts Read About Other Cloning Techniques Additional ... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog Post... smear across your FSH vs reporter plot. In this post, I’ll walk you through how to interpret the antibody... Note that for the flow plots discussed in this post, voltage has been adjusted appropriately and compensation... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post...hundred trillion connections (synapses.) In this post we’ll describe the AAV retrograde serotype (AAVrg...new tool, go forth and trace some neurons! This post was originally written by Leila Haery in 2017 and... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog Post...This post was contributed by Ignacio Amigo and María Hernández Sánchez from Crowdfight. In March 2020... resources on Addgene's blog Check out our blog post about remote collaborations Read our Transferable...science. Others are experienced researchers, such as postdocs and principal investigators. They also come from...IgnacioAmigoH María Hernández-Sánchez is a postdoctoral researcher at Instituto de Investigación Biomédica... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...to improve processes in the Addgene lab. In this post, I’ll tell you a little bit about what I learned... got to summarize my experience in my first blog post! I highly recommend doing the same if you ever go... -
A Deep Dive into BioBuilder
TypeBlog Post...This post was contributed by Mary Tamer from BioBuilder, an innovative nonprofit bringing the study of...Optogentics for E. coli Browse All Synthetic Biology Blog Posts Resources on Addgene.org Guide to SynBio Assembly...chapter and an illustrated, downloadable classroom poster — with both of these resources road tested over... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post...plasmids, glycoproteins and other proteins that require post-translational modification may not be easily expressed...the Addgene blog Read all of our COVID-19 blog posts Learn about other COVID-19 plasmids available at... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post.... To dive deeper into this field read our recent post “Cancer and the Immune System: Deciphering the Relationship...on the Addgene Blog Read Additional Cancer Blog Posts Addgene's Cancer Collection Pages Cancer and the...open-source! Impressions from the poster sessions During the poster session I got a chance to talk with...tumor microenvironment (one of the plasmids on her poster is pTomo-Ras which has been deposited at Addgene... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...Check out more synthetic biology posts! Check out more optogenetics posts! ... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog Post...questions. Starting at the Broad Institute For his post-doc, Doench worked on using RNAi screens to learn...others at the Broad Institute here Read other blog posts about CRISPR Learn more about the Broad Institute...