We narrowed to 361 results for: reporter

1. Important Considerations When Using AAVs

   Type

   Blog Post

   ... complexes to study neural function. Often the reporter proteins associated with these complexes are so...

2. Antibodies 101: Flow Cytometry

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   Blog Post

   ...tag your protein of interest with a fluorescent reporter on the N- or C-terminus of the protein. One downside...

3. Cancer and the Immune System: Deciphering the Relationship

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   Blog Post

   ...), and ability to metastasize. There have been reports of clinical trials going amiss and patient deaths...

4. Special Delivery: Fluorophore Targeting for FRET Studies

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   Blog Post

   ...protein kinase dynamics in living cells with FRET reporters." Methods 40.3 (2006): 279-286. PubMed PMID: 16908183...

5. Chemogenetics Guide

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   Guide

   ...control expression of a chemogenetic reporter ensures that the reporter remains silent until a cell or tissue-specific...

6. Validated gRNA Sequences

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   Collection

   ...pyogenes 23929339 Sheen pGL3-Basic-8x-gRNA-eGFP reporter synthetic AAAGGTCGAGAAACTGCAAA 60719 activate ...

7. Guide to Using Pooled Libraries

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   Guide

   ...die or do not pass selection (in the case of a reporter) Sort “winning” cells Sequence those cells that...

8. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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   Blog Post

   ...second. The first successful irreversible PA-FP reported was PA-GFP. PA-GFP was derived from Jellyfish ...

9. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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   Blog Post

   ...insulin-producing β-like-cells [37, 38]. In 1987, Davis et al. reported one of the earliest examples of transdifferentiation...

10. CRISPR 101: Cytosine Transversion Editors

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    Blog Post

    ...Adenine Base Editors Read about four base editor reporters CRISPR 101: Prime Editing CRISPR 101: Cas9 Structural...

11. Antibody Validation for Flow Cytometry

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    Blog Post

    ... specific detection of human TRPA1. Scientific Reports, 9(1), 18500. https://doi.org/10.1038/s41598-019...

12. Molecular Biology Reference

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    Guide

    ...information on CRISPR check out Addgene’s CRISPR guide . Reporter Plasmids - Used for studying the function of genetic... genetic elements. These plasmids contain a reporter gene (for example, luciferase or GFP) that offers...

13. AAV Purification by Iodixanol Gradient Ultracentrifugation

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    Protocol

    ...and unlike other density gradient media, it is reportedly not necessary to remove iodixanol prior to use...

14. CRISPR 101: Cytosine and Adenine Base Editors

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    Blog Post

    ...editing for plants Read about four base editor reporters Additional Resources on Addgene.org Catch up...

15. Troubleshooting and Optimizing a Western Blot

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    Blog Post

    ...blot, you may want to consider using a different reporter or, if you don’t need to compare the protein amounts...

16. Gamma-Retroviral Vector Guide

    Type

    Guide

    ...the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection marker...

17. Optogenetics Guide

    Type

    Guide

    ...optical switches Sensors are genetically-encoded reporters of molecular signals; e.g., calcium indicators...

18. Adenovirus Guide

    Type

    Guide

    ...Adenovirus Vector as a Zika virus vaccine . Scientific Reports, 10 (1), 3613. https://doi.org/10.1038/s41598-020...

19. Lentiviral Vector Guide

    Type

    Guide

    ...the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection marker...

20. Viral Vectors 101: Systemic Capsids

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    Blog Post

    ...expression from mouse to primate neocortex. Cell Reports, 34(13). https://doi.org/10.1016/j.celrep.2021.108754...