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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 369 results for: reporter

Showing: 341 - 360 of 369 results

  2. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...Integrase Gene Editing (PASSIGE), their previously-reported technique for precisely integrating large DNA ...

  3. Finding Your Science Policy Path

    Type
    Blog Post
    ...science policy skills in graduate school via this report from the Scientific Citizenship Initiative.    ...

  7. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...tag your protein of interest with a fluorescent reporter on the N- or C-terminus of the protein. One downside...

  9. CRISPR Guide

    Type
    Guide
    ...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman...mapping multiprotein complexes in human cells. Cell Reports . 13 (3), 621–633. PMID: 26456817 Doench, J. G....

  11. Validated gRNA Sequences

    Type
    Collection
    ...pyogenes 23929339 Sheen pGL3-Basic-8x-gRNA-eGFP reporter synthetic AAAGGTCGAGAAACTGCAAA 60719 activate ...

  15. Chemogenetics Guide

    Type
    Guide
    ...control expression of a chemogenetic reporter ensures that the reporter remains silent until a cell or tissue-specific...

  16. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    .... (2013). One-Step generation of mice carrying reporter and conditional alleles by CRISPR/CAS-Mediated...

  17. Guide to Using Pooled Libraries

    Type
    Guide
    ...die or do not pass selection (in the case of a reporter) Sort “winning” cells Sequence those cells that...

  18. Molecular Biology Reference

    Type
    Guide
    ...TALEN Plasmids and Kits , Genome Engineering Kits Reporter Plasmids Used for studying the function of genetic...genetic elements. These plasmids contain a reporter gene (e.g., luciferase or GFP) that offers a readout...
Showing: 341 - 360 of 369 results