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Showing: 361 - 380 of 420 results

  3. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...2025). In retrotransposon insertion, a combined endonuclease-reverse transcriptase uses homology arms flanking...

  11. DNA Quantification

    Type
    Protocol
    ...your substance maximally absorbs. In the case of nucleic acid (DNA and RNA), the maximal absorbance is at...Protein maximally absorbs at 280nm and the ratio of nucleic acid to protein (260/280) is generally used as ...

  14. Cre-lox system

    Type
    Collection
    ...65727 pcDNA3.1-CMV-CFP;UBC-Cre25nt Cre with a 25 nucleotide extracellular vesicle targeting sequence and ...Zeng 140135 pAAV-EF1a-iCreV Split Cre fused to nuclear localized wild-type VVD EF-1 alpha AAV Zeng 140284...

  16. Technical Design of a Western Blot

    Type
    Blog Post
    ...membrane-bound subcompartments, like the mitochondria or nucleus, will likely require a harsher lysis buffer than...

  17. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...Retrograde AAV: Making the journey from axon to nucleus Rabies and neuronal tracing Important considerations...

  18. Lentivirus ddPCR Titration

    Type
    Protocol
    ...targets. Primers and a probe against the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) are used as a control...primers/probe (HEX/VIC) 1 µL 9 µL 900 nM, 250 nM Nuclease-free water 4 µL 36 µL Total Volume 16 µL 144 µL...

  20. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes, it generates a double-strand...
Showing: 361 - 380 of 420 results