We narrowed to 732 results for: SON

1. Tag Your Favorite Yeast Genes with Ease

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   Blog Post

   ...systems have been developed in the labs of Anne Robinson, Eishi Noguchi, and Melissa Moore, to name a few...

2. Scientific Sharing in the Time of COVID-19: Databases and Resources

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   ... prioritizing research into this disease. A key reason this research has been able to progress so rapidly...

3. What's New in CRISPR - March 2020

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   ...CRISPRi plasmids A multiplexed gRNA piggyBac transposon system for human pluripotent stem cells Lindy...

4. New Norepinephrine Indicators: nLightG and nLightR

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   ... Rachel Leeson...

5. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

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   ... Rachel Leeson...

6. Addgene is Expanding Our Viral Vector Service!

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   ...  Rachel Leeson...

7. A Better Way to Get Customer Support: The Help Center

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   ...than ever to get the support you need. If for some reason you can’t find the answer that you’re looking for...

8. Future of Research Conference - Remarkable Opening Session

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   ...words of experience and encouragement.  Dr. Sibby Anderson-Thompkins, Director of the Office of Postdoctoral...

9. London Calling - Addgene’s New Europe Office in London

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   ...onset of spondylarthritis, using Bioluminescence Resonance Energy Transfer (BRET) technique, and plasmids...

10. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

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    ... O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang...

11. What's the Best Way to Elute and Store Your Plasmid DNA?

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    ...protocol to remove endonucleases activity. For these reasons choosing the correct solution is fundamental, and...

12. Plasmids 101: What is a plasmid?

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    ...restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning method is ultimately chosen ...

13. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

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    ...question is: how should the gRNAs be oriented in comparison to each other? The gRNAs must target different...

14. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

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    ...interactions for subsequent sheering of DNA by sonification. Though it helps capture weaker or more transient...

15. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

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    ...data about a disease or therapeutic Side-by-side comparison of the same experiment from different cell lines...

16. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

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    ...This series was written for selfish reasons: I wanted to learn about careers in science communication...

17. Some Like it Hot: Thermostable GeoCas9

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    ...optimal growth temperature of 55 °C. For a brief comparison of GeoCas9 vs. SpCas9, see the table below and...

18. SunTag and Fluorescent Imaging

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    ...the Vale and Weissman labs took this biological lesson and created a system to amplify fluorescent signals...

19. Tips for Improving Your Next Manuscript

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    ...practical understanding of how to apply it. The personal interaction of critiquing others’ manuscripts ...

20. Hot Plasmids and Viral Preps - July 2021

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    ...off-target effects.   New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

21. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

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    ...it from anywhere. Its ease of use is one of the reasons why this was the first program I used to create...

22. Bringing Sustainable Practices to the Lab: Recycling

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    ...easy to spot and even easier to ship back. Be that person! If you use serological pipettes from Corning...

23. Cellosaurus: A Cell Line Information Database

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    ...post was co-authored by Susanna Stroik and Rachel Leeson. Here at Addgene, we like to help share useful ...

24. FastCloning: A Newer, Simpler Cloning Technique

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    ...tips for applying it in your own lab. Table 1: Comparison Between Standard Restriction Cloning and Fast...

25. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

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    ...months down the line!) and to visit scientists in person to figure out ways we can make Addgene better and...

26. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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    ...found myself extremely grateful to my colleague Jason for choosing Simon Singh's "The Code Book" for this...

27. Choosing Your Perfect Empty Backbone

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    ... Lianna Swanson...

28. Advice for Choosing a Research Project

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    ...has multiple benchmarks. Think about what your personal goals are and set milestones along the way so ...

29. History of CRISPR Cas - A tale of survival and evolution

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    ...467-477. Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR.  Nature. 2013 Jan; 493, 429–432. Cong L,...

30. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    ...purification of tTALE-eGFP and tTALE-mCherry. In comparison to intercalating fluorescent dyes which can cause...