We narrowed to 643 results for: ato
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TypeBlog Post... one step further, you can inhibit cell cycle regulatory proteins responsible for the transition out of...
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Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post... in Parkinson’s disease. Knowing the primary investigator where the plasmid was created can narrow your... -
Plasmids 101: Protein tags
TypeBlog Post...proteins can be purified using metal-affinity chromatography, usually using a Ni2+ column. Like other affinity... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog Post...especially for young researchers. It was a really nice incubator for me. In Japan, of course, it is optimized for... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...optogenetics at OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking/selection... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog Post... the proper cell line is used; several common laboratory cell lines including 293 were immortalized with... -
To Codon Optimize or Not: That is the Question
TypeBlog Post...an organism that contains expression limiting regulatory elements or use a non-canonical code (Gustafsson... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post... and is commonly used to enrich adult mouse hematopoietic stem cells. Its ligand(s) has not been identified... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post...Einstein College of Medicine in the Department of Anatomy and Structural Biology, and now serves as the Director... -
Supporting Reproducibility with a Connected ELN
TypeBlog Post...researchers and PIs, science librarians and data curators, IT managers, the commercialization office, the... -
Plasmids 101: Shuttle Vectors
TypeBlog Post...antibiotic works. Another example is Zeocin, a DNA intercalator, which affects all kingdoms of life. Of course... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...seeing our resources shared via social media, on collaborators’ websites, or as a recommendation in a Reddit... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post... screen did fail to identify some known ricin-regulators, but most of these genes are also essential for... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...successfully to derive iPSCs from PBMCs. Most laboratories use Sendai virus to reprogram PBMCs efficiently... -
Bioinformatics at Addgene
TypeBlog Post...much information as possible from depositing laboratories and associated publications, but our quality... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post.... Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts in CRISPR evolution... -
Transferable Skills Guide: Creativity
TypeBlog Post...This is why perfectionists tend to also be procrastinators. If you fall into this category, get out of... -
Antibodies 101: Immunoprecipitation
TypeBlog Post...Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog Post.... The pDonor plasmid pRE57-Ter with multiple terminators can serve to interrupt genes (or gene clusters... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...fluorophore. The fluorescent signal can be used as an indicator to determine whether the target sequence is present... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog Post...several years ago – recently collaborated with Senator Barbara Boxer to introduce the Alan Reinstein Ban... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...paid. Institutional salary policies are one indicatator of how salaries may changes due to the FLSA. ... -
Transferable Skills Guide: Career Planning Resources
TypeBlog Post...and post-doctoral programs lack any formal or mandatory professional development training. Further, even... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express... -
Plasmids 101: Control Plasmids
TypeBlog Post...hypothesis: proper controls! For every experiment, an investigator needs a standard against which the results can... -
Lab Safety for Biosafety Levels One and Two
TypeProtocol... PPE protocols . Working in a BSL-2 laboratory requires laboratory glasses in addition to coat and gloves... eat, drink, chew gum, or apply makeup in the laboratory. Before you start your experiment, make sure ...Only mechanical pipetting should be done in the laboratory. Don’t mouth pipette! There may be some times...officer. BSL-2 Guidelines Remember, the BSL-1 laboratory guidelines above are expected to be followed ...not be worn outside of the BSL-2 area. BSL-2 laboratories must be clearly marked as “BSL-2.” The names...names and contact information of the laboratory manager should be clearly visible in the room and on the ...vaccination or titer prior to starting work in the laboratory. For some biohazardous waste, an autoclave or... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...10% expression in primary human T cells and hematopoietic stem and progenitor cells (HSPC)s to ~40% expression... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post...et al. "Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent... -
Hot Plasmids: Summer 2024
TypeBlog Post...dCas9, zinc finger proteins, and transcription activator-like effectors (TALEs), with fifteen total variants... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...