We narrowed to 933 results for: KIN

...round of evolution (AID-2) to reduce some of the leakiness associated with the system, but the general concept...

... experiments. Considerable effort has gone into making FPs monomeric, yet many investigators remain ignorant...

...found yourself bamboozled by all of the different kinds of information on our plasmid pages? Well, to help...

...complex trendlines. If you don’t have access to this kind of software, don’t give up! Rather than plotting...

...Multi-color labeling of neurons is for more than making pretty pictures (see figure 4). It also enables...

...phenotypic analysis it is appropriate to begin working in the T2 generation. The selection process itself...

... Addgene Blog Read this blog post about CRISPR Kinome Libraries available at Addgene Learn about the ...

...array. Workflow and technical considerations Making insertions in bacterial genomes with the V. cholera...

...resources References Bauzon, M., Castro, D., Karr, M., Hawkins, L. K., & Hermiston, T. W. (2003). Multigene expression...

...been limited, in part, due to the necessity for working with this virus under the highest biosafety level...

... version of TnpB is just 408 amino acids long — making it one of the most compact genome editors to date...

...choose the best method based on the plasmid. By working with the Data team, the QC team has also improved...

...assembly of the split luciferase is reversible, making PILATeS suitable for live imaging with a time resolution...

...through a second recombination event between the two flanking repeats. In cells where recombination has not ...

...neuron-specific promoters, including calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin...

...Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target, but many...

...APOBEC-Cas9 editosome would restore eGFP fluorescence, making it easy to quantify single base editing efficiency...

... repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or reverted...

...So, as long as it doesn't look done, just keep working on it. Also bear in mind that this is just a review...

...results can be compared; results from an experiment lacking the proper controls are invariably inconclusive...

...create double-stranded breaks in the DNA backbone, making them significantly less prone to indel mutations...

...Chartrand. "Using fluorescent proteins to study mRNA trafficking in living cells." Methods in cell biology 85 ...

.... Li, K et al. PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et...

...lamps are better, and xenon lamps are the best for working in this spectral range. You can also check that...

... you chose for your experiment, but, if you’re working with plants, don’t be afraid to try your hand at...

...low to no off-target activity at the ten highest-ranking predicted off-target sites. Each paper characterizes...

...2008). It can also cross the blood-brain barrier, making it useful for applications targeting the CNS (Issa...

...sites for conditional deletion of exon 6 of Syt1, mimicking a previously made mouse model. To avoid disrupting...

... using UV as it can cause damage to the eyes and skin. When running a gel for purification purposes it...

...inversion, and then lox sites excision, therefore locking the opsin into the correct orientation to be transcribed...

...general suggestions given by the International Working Group on Antibody Validation (Uhlen, et al., 2016...

...green), CARD14 (red), and pNFkB (blue) in lesional skin from a psoriasis patient, showing an inflammatory...

...induced pluripotent stem cells to mature functional Purkinje neurons. Sci Rep, 2015. 5: p. 9232. PubMed PMID...