We narrowed to 929 results for: KIN

1. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   ...were the only realistic options for scientists working in regenerative medicine, but the longing for a...

2. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   ... experiments. Considerable effort has gone into making FPs monomeric, yet many investigators remain ignorant...

3. Anatomy of a Plasmid Page at Addgene

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   ...found yourself bamboozled by all of the different kinds of information on our plasmid pages? Well, to help...

4. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   ...array. Workflow and technical considerations Making insertions in bacterial genomes with the V. cholera...

5. AAVs CREATed for Gene Delivery to the CNS and PNS

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   ...Multi-color labeling of neurons is for more than making pretty pictures (see figure 4). It also enables...

6. Great Results Start with Great Standard Curves

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   ...complex trendlines. If you don’t have access to this kind of software, don’t give up! Rather than plotting...

7. An Introduction to Adenovirus

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   ...resources References Bauzon, M., Castro, D., Karr, M., Hawkins, L. K., & Hermiston, T. W. (2003). Multigene expression...

8. Tips for arabidopsis transformation

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   ...phenotypic analysis it is appropriate to begin working in the T2 generation. The selection process itself...

9. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ... Addgene Blog Read this blog post about CRISPR Kinome Libraries available at Addgene Learn about the ...

10. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    ...been limited, in part, due to the necessity for working with this virus under the highest biosafety level...

11. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ...Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target, but many...

12. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    ...APOBEC-Cas9 editosome would restore eGFP fluorescence, making it easy to quantify single base editing efficiency...

13. New Tools Enable CRISPRa for Neuroscience Applications

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    ...neuron-specific promoters, including calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin...

14. CRISPR-mediated Plant Base Editors

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    ... repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or reverted...

15. How to Write a Scientific Review Article

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    ...So, as long as it doesn't look done, just keep working on it. Also bear in mind that this is just a review...

16. Hot Plasmids: Fall 2024

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    ...assembly of the split luciferase is reversible, making PILATeS suitable for live imaging with a time resolution...

17. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    ...through a second recombination event between the two flanking repeats. In cells where recombination has not ...

18. Plasmids 101: Control Plasmids

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    ...results can be compared; results from an experiment lacking the proper controls are invariably inconclusive...

19. Fluorescent Proteins 101: When GFP lets you down

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    ...Chartrand. "Using fluorescent proteins to study mRNA trafficking in living cells." Methods in cell biology 85 ...

20. Quick Guide to Near-Infrared Fluorescent Proteins

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    ...lamps are better, and xenon lamps are the best for working in this spectral range. You can also check that...

21. CRISPR 101: Homology Directed Repair

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    .... Li, K et al. PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et...

22. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    ... you chose for your experiment, but, if you’re working with plants, don’t be afraid to try your hand at...

23. Treating Muscular Dystrophy with CRISPR Gene Editing

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    ...low to no off-target activity at the ten highest-ranking predicted off-target sites. Each paper characterizes...

24. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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    ...sites for conditional deletion of exon 6 of Syt1, mimicking a previously made mouse model. To avoid disrupting...

25. Plasmids 101: Restriction Cloning

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    ... using UV as it can cause damage to the eyes and skin. When running a gel for purification purposes it...

26. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    ...inversion, and then lox sites excision, therefore locking the opsin into the correct orientation to be transcribed...

27. Antibodies 101: Validation

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    ...general suggestions given by the International Working Group on Antibody Validation (Uhlen, et al., 2016...

28. Antibodies 101: Introduction to Antibodies

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    ...green), CARD14 (red), and pNFkB (blue) in lesional skin from a psoriasis patient, showing an inflammatory...

29. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    ...induced pluripotent stem cells to mature functional Purkinje neurons. Sci Rep, 2015. 5: p. 9232. PubMed PMID...