We narrowed to 382 results for: BRE

1. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

   Type

   Blog Post

   ...the basics of Gibson assembly as a parody of “Breakfast at Tiffany’s.” Overview of Gibson assembly The...

2. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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   Blog Post

   ...distinct cell populations. To decrease the animal breeding necessary to produce animals with Brainbow transgenes...

3. Hot Plasmids: Spring 2025

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   Blog Post

   ...resistance gene makes stable cell line creation a breeze. Modular by design: Key elements (promoter, tag...

4. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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   Blog Post

   ...1, rAAV2/2, and so on, although we often use abbreviated nomenclature (AAV1, AAV2, etc.) to distinguish...

5. Viral Vectors 101: AAV Variables That Matter

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   Blog Post

   ...., Hu, H., Lee, Y., D’Azzo, A., Messing, A., & Brenner, M. (2004). Expression specificity of GFAP transgenes...

6. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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   Blog Post

   ...incredibly useful technique simply by following the abbreviated methods section found within a published manuscript...

7. Technique: Probe Phage Genomes for Host Binding Proteins

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   Blog Post

   ...her on Twitter @JessicaSacher.   References 1. Nobrega, Franklin L., et al. "Revisiting phage therapy:...

8. Plasmids 101: Restriction Cloning

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   Blog Post

   ...of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.   ...

9. Finding Your Perfect Job After University

   Type

   Blog Post

   ...was hard; I was researching the epidemiology of breast cancer and I loved the practical aspects of testing...

10. Building Global Connections with the International Mentorship Program USA-EUROPE

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    Blog Post

    ... IMFAHE, Inc. She is particularly interested in breast cancer and metastasis as well as teaching and mentoring...

11. Plasmids 101: Using Transposons in the Lab

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    Blog Post

    ...terminal repeats (LTRs), induces a double-stranded break, and excises the transposon from the donor DNA. ...

12. A Guide to Getting Started in Undergrad Research

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    Blog Post

    ...basket. There are many factors that can make or break a lab experience. Previous Addgene blogs have discussed...

13. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    ...talk about it. DNA ligases are enzymes that seal breaks in DNA by joining 5 ́-phosphorylated DNA termini...

14. Quick Guide to All Things Lentivirus

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    Blog Post

    ... find will recommend using a polycation, like polybrene (protocol) or DEAE dextran (protocol), which help...

15. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post

    ...PMCID: PMC5024581. 15. Celli, Susanna, Béatrice Breart, and Philippe Bousso. "Intravital two-photon imaging...

16. Molecular Biology Reference

    Type

    Guide

    ...bases, adenine, thymine, cytosine, and guanine (abbreviated to A, T, C, and G respectively) that are organized...process of molecular cloning enabled scientists to break chromosomes down to study their genes, marking the...series covers topics ranging from the newest breakthroughs in plasmid technologies and research, to overviews...

17. Plan Your Experiment

    Type

    Guide

    ... specific base pair edits without double-strand breaks Base editor (dCas9 or Cas9 nickase fusions with... a homology template, a large percentage of DNA breaks will be repaired by NHEJ instead of incorporating...

18. Promoters

    Type

    Guide

    ...series , covers topics ranging from the newest breakthroughs in plasmid technologies and research to overviews...

19. Cloning

    Type

    Guide

    ...protocol for restriction cloning and an in-depth breakdown of restriction digests on our website. Restriction...

20. Gamma-Retroviral Vector Guide

    Type

    Guide

    ...v3060677 PMID: 21994751 Mekkaoui, L., Tejerizo, J. G., Abreu, S., Rubat, L., Nikoniuk, A., Macmorland, W., Horlock...