We narrowed to 703 results for: ESC;

...A recent publication [1] from the Sánchez lab describes the plasmids and protocols required for both WISH... which are available through the deposit) are described in the Sánchez lab publication [1] so that the...

...dividing and non-dividing cells. More detailed descriptions of the lentiviral elements and the 2nd and 3rd... deletion (Cre), together with expression of fluorescent or enzymatic reporters for cellular assays and...

...for binding to the target protein. The protocol describes going through two rounds of phage display to increase...interest. The Seeger lab used the ELISA technique described in their protocol because it is less susceptible...

...visualize proteins in vivo by tagging them with fluorescent proteins to study localization or purify proteins...Expression Systems Recombinant protein expression in Escherichia coli: advances and challenges Production of recombinant...

...Center are now available at Addgene along with a description of their experimental protocol. The three plasmids...systems also work equally well in dividing and quiescent, post-mitotic mammalian cells. Applications of...

...Building on the lab’s previous work, Sakuma et al. describe a detailed protocol for MMEJ-mediated knock-in... transgenes. It’s important to note that the fluorescence level observed will be dependent on both the...

...revealed that cell lines from individuals of African descent have approximately 20% more single nucleotide polymorphisms...are more common in individuals of African descent.Correcting the bias  While their thorough analysis uncovered...

...into two components: 𝜃x(t) and 𝜓z(t). 𝜃x(t) describes the exponential decay process we observed, while...model for each region using stochastic gradient descent in Pytorch, which gave us the maximum likelihood...

...region In 2013, the Hodaka Fujii Laboratory first described a method to purify a specific genomic region using...plasmid. Experimental use Since the initial report describing CRISPR-mediated purification of a specific genomic...

...targeted approach using RNPs has recently been described in vitro, with the hope of eventually using this...is required, such as when a Cas9 fusion to a fluorescent protein is used to label nucleic acid targets...

...variations, including many that use auxotrophic rescue genes as reporters and some that repress instead...gene fusions for analyzing gene expression in Escherichia coli and yeast. Methods Enzymol. 1983;100:293...

...VSV-G is also toxic at high concentrations as described above and is inactivated by the complement system...proteins that can be used for specific purposes as described previously (Table 1, Gutierrez-Guerrero, et al...

...quantitative reporters and use a substrate to produce bioluminescence. Luciferases usually have shorter mRNA and ...tNOS & pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS. Fluorescent proteins (FP, e.g. GFP) can be used in many ways...

...plasmids in our inventory from the lab of Eric Wieschaus that express sqh.   cookie monster, a meiotic...1905980. Nüsslein-Volhard, Christiane, and Eric Wieschaus. "Mutations affecting segment number and polarity...

...calendar invite over to the r/Science team with a description of our AMA and Joanne’s preferred time to start...receive via email. The cool features are too many to describe here, but I have over 2,000 notes which Evernote...

...Overgrown cultures stimulate quiescence, the resting phase of the cell cycle; quiescent cells do not take up ...

...plasma membrane. In previous publications, they described tools to control formation of lamellipodia and... unnatural head groups for applications like fluorescent labeling via click chemistry.    Figure 4: SuperPLD...

...feedback. Many of these documents are too vaguely described in other sources of guidance or the document examples...in the Endoplasmic Reticulum, optimization of fluorescent proteins,  and live cell imaging approaches. ...

...listeners to be thinking “What happens next?”  Describe how you pushed to a solution or overcame obstacles...term, but are worried that this might sound condescending, consider explaining the term on a slide but...

..., and ultraviolet light. In this post, we will describe the mechanism of homology directed repair, one...generally preferred for large insertions such as fluorescent proteins or selection cassettes. This class of...