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We narrowed to 637 results for: ESP

Showing: 361 - 380 of 637 results

  1. Technical Design of a Western Blot

    Type
    Blog Post
    ...primary antibodies can be more difficult to source, especially if you need a specific conjugate, and changing...detection quickly (~1 minute), and have a linear response over a broad dynamic range that typically spans...

  2. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ... protein increases (Figure 3A).  This step is especially important for highly expressed proteins, including... with biorender.com.    Since antibodies, especially polyclonal antibodies, can vary lot-to-lot, this...

  3. Adenovirus Guide

    Type
    Guide
    ...disease. Innate immunity Non-specific immune response that responds to general signals of infection or damage... line are in forward or reverse orientation, respectively. Created with BioRender.com. Adenoviruses are...diseases in humans and other animals, most commonly respiratory infections, such as the common cold. The immune...elements rapidly and can mount a strong immune response quickly, which has limited their use in therapeutic... rAdV vectors were shown to elicit less immune response when administered in vivo, however deletion of...Addgene #16400) is suitable for most purposes. For especially long transgenes, the use of pAdEasy-2 (Addgene.... Due to this, and to the strong host’s immune response against the transduced cells, transgene expression...

  4. Chemogenetics Guide

    Type
    Guide
    ...chemogenetic tools are unresponsive to native ligands and are engineered to respond to small molecules that...PSAM that responded to a clinically approved drug, the PSAM domain was engineered to respond specifically... development of mutated receptors that while unresponsive to endogenous ligands, could be activated by...Gs-DREADD, rM3D, that was created by replacing the corresponding intracellular region of a turkey erythrocyte...Muscarinic-receptor based DREADDs were engineered to respond to nM concentrations of clozapine N ‐oxide (CNO... shown to be an agonist against hM4Di, and is especially attractive for use in translational studies because...of a suite of muscarinic-based DREADDs that all respond to the same ligands was that bidirectional experiments...

  5. New Antibody Animation!

    Type
    Blog Post
    ... what antibodies are, their role in the immune response, and the different antibody isotypes found in ...

  6. Antibody Guide

    Type
    Guide
    ...animal, which then mounts an immune response to it. Once the immune response has developed, the antibodies ...Antibodies, produced as part of the immune system’s response to foreign bodies, are the most commonly used ...Figure 1. The two arms of the Y structure are responsible for binding the antigen, and are called the antigen...bind to a specific antigen, triggering an immune response. The stem of the Y structure and the lower part...isotype of the antibody. B cells, the immune cell responsible for producing antibodies, can change the constant...binding site. In B cells, the genetic region responsible for the variable region goes through a randomized...Different isotypes play different roles in the immune response, and can be used to increase flexibility or specificity...

  18. Optogenetics Guide

    Type
    Guide
    ... activation by light, these channels and pumps respond by opening or closing, which conducts the flow ...variety of naturally occurring microbial opsins that respond to different wavelengths of light, like blue or...opsins also initiate different electrochemical responses, such as nonspecific cation influx vs. proton ... used to trigger and study certain behavioral responses in model organisms like mice, zebrafish, and Drosophila...Opsin Variants Opsin Type Variant Description Peak Response Spectra (nm) Channelrhodopsins: cation channels...you’d pick an excitatory or inhibitory opsin, respectively. Color of activation light. There are a variety...expression of the opsin is reached. This approach is especially powerful when combined with site specific recombinase...

  19. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ... AAV Packaged on Request  has seen a fantastic response from the research community. Customers have taken...
Showing: 361 - 380 of 637 results