We narrowed to 671 results for: FIE

...cells. FPs classified by the emission color (emission wavelength range) FPs are usually classified by emission...

... termini. It doesn’t matter if the DNA is PCR amplified, restriction digested or even a synthesized gene... are assembled in vivo to produce the multiply modified product. For example, by combining multiple primers...

...you than your technical expertise in a specific field. While searching for jobs and making connections...identify it as a personal strength until it is identified by another person. Similar reasoning may also...

...barrier - only those that cross the barrier can be identified by PCR later. The PNS presents a different problem...out tables 1 and 2 below for a summary of the quantified transduction efficiency of AAV-PHP.eB and AAV-PHP.S...

... global activity in our particular scientific subfields.  Plasmid requests correlate with paper publication...The effects of COVID-19 on many other scientific fields could be different.  Conclusion Figure 7:...

...You have just purified your plasmid and are ready to move on to your downstream application — but wait...blue lines in B-D represent the spectra for the specified contaminant, while the grey lines in B-D represent...

...now. When titrating AAV by qPCR, viral DNA is amplified and monitored in real time. When the PCR is complete...each one. Droplets that fluoresce contain the amplified target sequence. After all of the droplets have...

...Francisella), was annotated in several genomes and is classified as a type V CRISPR system. Like Cas9, Cpf1 contains...and off-target efficiency When Cpf1 was first identified, we didn't know much about its on-target and ...

...Secondly, 3rd generation packaging systems have modified the 5’ LTR of the transfer plasmid to eliminate...cellular genes. Q7: Which genes are deleted or modified to result in replication deficiency? What percent...

...and attR sites.   Gateway vectors contain modified versions of the att sites so that scientists can...thanks to the Gateway vectors’ design. They have modified versions of the attB, P, L and R sites that recombine...

...method with Agrobacterium: If you are new to the field, the following are useful tips for your first transient... Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based transformation method for...

... must be screened to identify effective ones. Simplified in vivo delivery using SPH mice CRISPRa has largely...mouse (Zhou et al., 2018). This mouse expresses a modified version of the “Suntag” Cas9 activator that recruits...

...Kleinstiver et al. 2015). From these screens, they identified four novel SpCas9 variants with altered PAM binding... be used to modify genomic loci that cannot be modified using wild-type SpCas9. The number of off-target...

...sequence-specific protease used to cleave affinity tags from purified recombinant proteins at small and large scale....biotinylation. Once induced, the NBs could be easily purified by one-step immobilized metal ion adsorption chromatography...

...editing by APOBEC-Cas9 Though the ACE reporter quantifies APOBEC-Cas9 efficiency in real-time, it relies...reporters- eGFP L202, eGFP L138, and eGFP L93. The modified eGFPs were placed downstream of wild-type mCherry...

...quantities of the target!) and use them in numerous fields including research and development, diagnostics...could be an endogenous sample of wild type cells, purified protein, or a peptide. The positive control will...

...sequencing also relies on sequencing by synthesis, but modifies the original Sanger procedure to improve throughput... base identity and length, but disregards the specified discrepancies. Any sequences that are a perfect...

...experimental purposes. But what now? MoClo really simplifies vector construction by combining restriction ...process to produce a multigene construct. Figure exemplifies the extraction and use of a coding sequence of...

... microscopy, GVs visible in cells via TEM, and purified GVs under TEM. (c) Ultrasound imaging of tumor...231 cells (top, scalebar = 10µm) and TEM of GVs purified from MDA-MB-231 cell lysate after 4-days of expression...

...Meeting seven other times I had subsequently changed fields and my cohort of friends and colleagues had moved...effort in the future to match people within similar fields (ASM Microbe has eight tracks), something we didn...