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  2. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...barrier - only those that cross the barrier can be identified by PCR later. The PNS presents a different problem...out tables 1 and 2 below for a summary of the quantified transduction efficiency of AAV-PHP.eB and AAV-PHP.S...

  3. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ... termini. It doesn’t matter if the DNA is PCR amplified, restriction digested or even a synthesized gene... are assembled in vivo to produce the multiply modified product. For example, by combining multiple primers...

  5. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...now. When titrating AAV by qPCR, viral DNA is amplified and monitored in real time. When the PCR is complete...each one. Droplets that fluoresce contain the amplified target sequence. After all of the droplets have...

  6. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...Francisella), was annotated in several genomes and is classified as a type V CRISPR system. Like Cas9, Cpf1 contains...and off-target efficiency When Cpf1 was first identified, we didn't know much about its on-target and ...

  7. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...Secondly, 3rd generation packaging systems have modified the 5’ LTR of the transfer plasmid to eliminate...cellular genes. Q7: Which genes are deleted or modified to result in replication deficiency? What percent...

  8. Lentivirus Production

    Type
    Protocol
    ...) transfection protocol. This procedure can be modified for alternative packaging cell lines or transfection...without limiting cell growth. Left panels: bright field images; right panels: GFP channel images. Last reviewed...

  9. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...and other online career resources Once you’ve identified places you’d like to work, get online and take... a personal connection. That way, once you’ve identified an appropriate job opening, you can send your...

  10. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...method with Agrobacterium: If you are new to the field, the following are useful tips for your first transient... Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based transformation method for...

  11. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ... must be screened to identify effective ones. Simplified in vivo delivery using SPH mice CRISPRa has largely...mouse (Zhou et al., 2018). This mouse expresses a modified version of the “Suntag” Cas9 activator that recruits...

  12. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...and attR sites.   Gateway vectors contain modified versions of the att sites so that scientists can...thanks to the Gateway vectors’ design. They have modified versions of the attB, P, L and R sites that recombine...

  13. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Kleinstiver et al. 2015). From these screens, they identified four novel SpCas9 variants with altered PAM binding... be used to modify genomic loci that cannot be modified using wild-type SpCas9. The number of off-target...

  14. Hot Plasmids - August 2020

    Type
    Blog Post
    ...sequence-specific protease used to cleave affinity tags from purified recombinant proteins at small and large scale....biotinylation. Once induced, the NBs could be easily purified by one-step immobilized metal ion adsorption chromatography...

  17. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...quantities of the target!) and use them in numerous fields including research and development, diagnostics...could be an endogenous sample of wild type cells, purified protein, or a peptide. The positive control will...

  19. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...into the BL21(DE3)/pCas strain. In response, we modified the pCas/pTargetF system and updated it into a...Sun B, Chen J, Zhang Y, Jiang Y, Yang S (2021) A modified pCas/pTargetF system for CRISPR-Cas9-assisted ...
Showing: 361 - 380 of 665 results