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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 672 results for: FIE

Showing: 361 - 380 of 672 results

  2. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...developed using a stereomicroscope to capture large field-of-views (6). For more comprehensive reviews on ... alcohol and methyl salicylate which was later modified by others to produce Murray’s clear solution.  ...

  4. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...has been optimized for adherent cells but can be modified for suspension cells. The number of monoclonal...the cells are overly confluent or the medium is acidified (i.e., the medium appears orange/yellow if using...

  5. Kit Free RNA Extraction

    Type
    Protocol
    ...protocol for DNA quantification , which can be modified for RNA. Store your RNA sample(s) at -80 °C to...protocol for DNA quantification , which can be modified for RNA.Store your RNA sample(s) at -80 °C to ...

  7. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...now. When titrating AAV by qPCR, viral DNA is amplified and monitored in real time. When the PCR is complete...each one. Droplets that fluoresce contain the amplified target sequence. After all of the droplets have...

  10. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ... termini. It doesn’t matter if the DNA is PCR amplified, restriction digested or even a synthesized gene... are assembled in vivo to produce the multiply modified product. For example, by combining multiple primers...

  11. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...barrier - only those that cross the barrier can be identified by PCR later. The PNS presents a different problem...out tables 1 and 2 below for a summary of the quantified transduction efficiency of AAV-PHP.eB and AAV-PHP.S...

  12. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...Francisella), was annotated in several genomes and is classified as a type V CRISPR system. Like Cas9, Cpf1 contains...and off-target efficiency When Cpf1 was first identified, we didn't know much about its on-target and ...

  13. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...Secondly, 3rd generation packaging systems have modified the 5’ LTR of the transfer plasmid to eliminate...cellular genes. Q7: Which genes are deleted or modified to result in replication deficiency? What percent...

  14. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Kleinstiver et al. 2015). From these screens, they identified four novel SpCas9 variants with altered PAM binding... be used to modify genomic loci that cannot be modified using wild-type SpCas9. The number of off-target...

  15. Hot Plasmids - August 2020

    Type
    Blog Post
    ...sequence-specific protease used to cleave affinity tags from purified recombinant proteins at small and large scale....biotinylation. Once induced, the NBs could be easily purified by one-step immobilized metal ion adsorption chromatography...

  18. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...and other online career resources Once you’ve identified places you’d like to work, get online and take... a personal connection. That way, once you’ve identified an appropriate job opening, you can send your...

  19. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...sequencing also relies on sequencing by synthesis, but modifies the original Sanger procedure to improve throughput... base identity and length, but disregards the specified discrepancies. Any sequences that are a perfect...
Showing: 361 - 380 of 672 results