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We narrowed to 856 results for: HA

Showing: 361 - 380 of 856 results

  1. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...antibody is biotin-conjugated, streptavidin (which has a high affinity for binding biotin), is conjugated...detecting one protein from the sample. A direct ELISA has many fewer steps than a sandwich or competitive ELISA...linear portion or the OD of your experimental samples has reached 4.0, you can resolve this issue when you ...

  2. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...called the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent...visualize genomic loci in vivo The flexibility of CRISPR has improved our ability to target almost every genomic... system and induce genome editing. Such a system has been previously described for transcriptional activation...

  3. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...CombiGEM-CRISPR: The marriage of two simple platforms CRISPR has revolutionized how we decode the genome by making...guide RNAs (gRNAs) for genome editing in large-scale has led to the rapid generation of genome-wide gRNA libraries...the users’ specific research questions. CombiGEM has been successfully applied to functionally characterize...

  4. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...auditory neuronal circuitry in vivo. A variant of RABV has been developed to only label a neuronal connection...example only dopaminergic neurons, neuronal tracing has the added benefit of only labelling the subset of... regions used. Evidence for retrograde transport has been found for AAV1, -2, -5, -7, -8 (Taymans et al...AAV5 (Aschauer et al., 2013). Anterograde transport has been observed for AAV1, -5, -8 (McFarland et al.,...variability compared to chemical tracers. rAAV2-retro also has lower affinity for heparin, which possibly explains...vectors cover some of these attributes and their use has allowed for the visualization of single connection...intracerebral application, which is more invasive and has a much higher risk of complications. References...

  5. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...IgG2a  Rat   Targeting integrins in the lab The Ha and Springer labs recently showed RGD-binding integrins... interpret the roles of individual integrins. The Ha lab — in collaboration with the Springer lab, the...Leucine-Valine-Aspartate-Proline (LVDP) motif. The Ha and Springer teams employed IPI synthetic antigen-binding... J., Yan, J., Waterman, C. M., Springer, T. A., & Ha, T. (2022). Single-molecule characterization of subtype-specific...

  6. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...in the same field that I do: prostate cancer. She has guided me in my next steps. For example, how to get...to travel abroad, how to write cover letters, and has suggested important things to emphasize in order ...insecurities… all shrink when shared with a mentor who has experienced them before and who can serve as a role...universities and future generations of professionals has made possible a significant qualitative and quantitative...

  7. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...lentiviral service is going strong, the AAV service has shown incredible growth this year. pAAV-hSyn-DIO-...pmSyn1-EBFP-Cre from Hongkui Zeng’s lab. This AAV has had over 150 orders since coming online! pmSyn1-EBFP-Cre...

  8. Lentivirus Production

    Type
    Protocol
    ...10 min and then recheck the pH to ensure that it has not drifted. Filter the solution through a 0.22 μm...

  9. Colony Formation Titering Assay

    Type
    Protocol
    ...mL) x dilution factor e.g., If the 1:100,000 well has 75 colonies, then there are 75 colonies /1.5 mL x...

  10. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...community has always emphasized the need for open science and collaboration. The field already has comprehensive...first virtual organism in a computer.” This project has produced various resources for the worm community...

  11. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...Cas12a. This is in part due to the fact that it only has a single active site. The RuvC active site in Casɸ...rely on multiple active sites. In addition, Casɸ has a minimal PAM requirement. For instance, for Casɸ...library here A new SaCas9 ABE variant (microABEI744) has improved on-target editing efficiency and reduced...

  12. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...the idea that DNA methylation is linked to disease has now become much like the 1980s song “Don’t Stop Believin...none existed. Recently, however, a particular tool has gained widespread popularity – and notoriety – as...may later be excised by Cre recombinase. Addgene has several great options: pLV hUbC-dCas9-T2A-GFP for...

  13. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...a look at the secondary antibodies that your lab has on hand and choose a primary antibody that will work...secondary antibody pairing such that each target has a unique species or isotype that can be probed with...polyclonal primary antibodies.  I hope this post has explained when and why antibody isotypes matter and...

  14. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...version of PubMed.  Key features This new version has an improved search algorithm which was trained on...your search terms. Key features Semantic Scholar has several ways to focus your search results. A bar ...paper’s entry, the number of citations that paper has is displayed at the top of the page, as well as the...

  15. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    ... a raise in salary, particularly given that this has precipitated increases to the NIH recommended pay...both of these changes share a common thread that has the potential to profoundly shift the academic science...rather than as trainees in a special category, as has been the ‘labor-of-love’ philosophy of academic science...

  16. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...Epigenetics has recently been hitting the headlines, with sotires like the potential devastation of the...them. Global hypomethylation, on the other hand, has also been implicated in the development and progression...difficult to overcome until now. Alice Ting’s lab has developed a new, plasmid-based biosensor to visualise...

  17. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...    In the video above, Channelrhodopsin 2 (Chr2) has been expressed in the motor cortex of a mouse. Upon...appropriate wavelength to stimulate your opsin. Each opsin has an excitation spectrum (use the resources discussed...controlled. As discussed above, each opsin variant has slightly different activation/inactivation kinetics...

  18. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ...your supervisor’s expectations Every supervisor has their own management style. A hands-on supervisor...agree with all of your ideas, but he or she probably has reasons for it. Do not take criticism personally ... of Finish Your Thesis, an online community that has supported thousands of graduate students since 2009...

  19. Editor's Choice, August 2016

    Type
    Blog Post
    ... Our Editor's Choice Blog Posts The Addgene Blog has had quite the up-tick in views over the month of ...recommend taking a look at all of our posts as each one has something great to offer, I just need to get back...

  20. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...the group? Has it grown over time? If not, why not? It may not be a bad thing if the lab has stayed that...purpose, but it could also be a sign that the lab head has trouble attracting scientists. To get a better idea...intervenes if there are interpersonal conflicts PI has no idea who is dating whom Expanding your skillset...
Showing: 361 - 380 of 856 results