Addgene

We narrowed to 400 results for: HRE

Showing: 361 - 380 of 400 results

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New Optogenetic Tools for Cytoskeleton and Membrane Control

Type

Blog Post

...Lab used such a strategy to dissect the roles of three Rab proteins during a key stage of zebrafish development...
CRISPR 101: Homology Directed Repair

Type

Blog Post

...sister chromatid, plasmid, etc.), and consists of three pathways: classical double-strand break repair (...
CRISPR 101: Validating Your Genome Edit

Type

Blog Post

... insertion or deletion that isn’t a multiple of three will do. A popular screening method for knockouts...
Antibodies 101: Normalization and Loading Controls for Western Blots

Type

Blog Post

...probed with β-actin (1:2000). Representative of three independent blots. B–C) Graphs showing the relative...
Transferable Skills Guide: Time Management

Type

Blog Post

...processes, I’ve broken down time management into three conceptual chunks: prioritization, planning, and...
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Type

Blog Post

..., Gao C, Qiu J-L (2014) Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable...
Evolution of Lab Techniques

Type

Blog Post

...technological innovations. Let’s take a look at three historical techniques: southern blotting, restriction...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Type

Blog Post

... Essentially, the immune system doesn’t see any threat to which it should respond. Latently infected cells...
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

Type

Blog Post

...Quadros et al. saw success with Easi-CRISPR across three independent core facilities for 13 projects, with...
Viral Vectors 101: Optogenetic Tools

Type

Blog Post

...Optogenetic tools Types of control We will discuss three types of tools: light-gated ion channels, optogenetic...
Antibodies 101: Multiplex Immunofluorescence

Type

Blog Post

...Whether you’re just getting started with two or three targets or you’re pushing the limits like Addgene...
PiggyBac-ing Through the Genome Editing Field

Type

Blog Post

...particularly useful when the gene being expressed has threshold effects, such as with signaling molecules or transcription...
RNA Interference in Plant Biology: New Tools for an Old Favorite

Type

Blog Post

.... A typical RNAi experiment can be divided into three fundamental steps: creation of the RNAi trigger...
Mycoplasma Contamination: Where Does It Come From and How to Prevent It

Type

Blog Post

...should not have been changed for at least two or three days before sampling (Uphoff and Drexler, 2011)....
Antibodies 101: Validation

Type

Blog Post

...you are aiming to achieve. In general, these are three core goals of antibody validation: Confirm reactivity...
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

Type

Blog Post

...Vectors Important Considerations When Using AAVs Three Key Considerations For Precise Neuronal Targeting...
Sequencing Options for CRISPR Genotyping

Type

Blog Post

...2010). As the field moves toward standardized thresholds for validating CRISPR experiments, many are turning...
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

Type

Blog Post

...technique was first Introduced to immunology by three papers in the journal Science in 2002. Now, this...
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

Type

Blog Post

...Jellyfish (Aequorea victoria) GFP (wtGFP) by mutating Threonine 203 to Histidine (T203H) (1). wtGFP normally contains...
Cancer and the Immune System: Deciphering the Relationship

Type

Blog Post

...progression. Dunn et al in 2002, introduced the “Three Es of Immunoediting” nomenclature. 1. Eliminate ...

Showing: 361 - 380 of 400 results

Results per page:

New Optogenetic Tools for Cytoskeleton and Membrane Control

CRISPR 101: Homology Directed Repair

CRISPR 101: Validating Your Genome Edit

Antibodies 101: Normalization and Loading Controls for Western Blots

Transferable Skills Guide: Time Management

CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Evolution of Lab Techniques

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

Viral Vectors 101: Optogenetic Tools

Antibodies 101: Multiplex Immunofluorescence

PiggyBac-ing Through the Genome Editing Field

RNA Interference in Plant Biology: New Tools for an Old Favorite

Mycoplasma Contamination: Where Does It Come From and How to Prevent It

Antibodies 101: Validation

A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

Sequencing Options for CRISPR Genotyping

Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

Cancer and the Immune System: Deciphering the Relationship

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