We narrowed to 433 results for: Hal

1. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   Blog Post

   ...for instance in inherited disorders such as B-thalassemia, Sickle Cells Disease, Duchenne Muscular Dystrophy...

2. Plasmids 101: Optimizing Plasmid Yields

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   ...and can therefore be cultured in media containing half the normally recommended antibiotic concentration...

3. PITChing MMEJ as an Alternative Route for Gene Editing

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   ...cell lines can be created in about a month and a half, without the need for complicated cloning of homology...

4. Plasmids 101: Multicistronic Vectors

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   ...segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes...

5. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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   ...fluorescent proteins. Visualizing fluorescence can be challenging in plants because of the auto-fluorescence of...

6. Great Results Start with Great Standard Curves

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   ...allergy testing lab, and the grad student down the hall from you have in common? All of them rely on standard...

7. An Introduction to Adenovirus

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   ... like other viral vectors, though significant challenges remain (Wold & Toth, 2013).  While the pre-existing...

8. SciComm: Taking Science to Elementary and Middle Schools

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   ...much in the making. One of the exciting, and challenging, aspects of talking to 8th graders was the broad...

9. Summer SciComm Series: Modes of Communication

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   ...graphics for visuals. However, it’s much more challenging to design a hands-on interactive activity using...

10. AAVs in Retinal Gene Therapy

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    ... completing her PhD and medical training in Ophthalmology. Gene therapy was in its infancy, but the eye...

11. Transferable Skills Guide: Identifying Your Transferable Skills

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    ...presents itself even outside of work, consider challenging yourself, take the opportunity, and discover ...

12. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...also theoretically possible, but likely more challenging, with lentiviral expression. For more detail ...

13. A Guide to Starting Your Own Journal Club

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    ...success” of the journal club. This can be a challengingly vague metric but there are a few things you ...

14. Tips for arabidopsis transformation

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    ... method of identifying transformed Arabidopsis thaliana seedlings following floral dip transformation’...

15. Simplify Cloning with in vivo Assembly

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    ... to amplify away from the insertion site. Encode half the new sequence at the 5’ end of each primer, with...

16. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    ... include: Self-labeling tags (e.g., SNAP-tag, HaloTag, TMP-tag) – These widely used systems consist of...

17. Tips for Screening with Yeast Two Hybrid Systems

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    ...functional luciferase only when fusions of the two halves of the luciferase protein interact and enable intein...

18. A Career in Grants: How To Become a Grant Professional

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    ...Becoming a grant professional is rewarding and challenging!  Jo Miller, GPC, CSMS is the founder and principal...

19. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    ...and when they come into close proximity, the two halves of EGFP undergo folding, maturation, and fluorescence...

20. Twenty Years of Sharing: Expanding Accessibility

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    ...persisted despite our best efforts. With a little over half of our orders coming from researchers outside the...