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We narrowed to 933 results for: Kin

Showing: 361 - 380 of 933 results

  1. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...protein AraC binds O and I1 sites upstream of pBad, blocking transcription. The addition of arabinose causes... cAMP and represses pBad, decreasing promoter leakiness.   Other examples of chemically induced promoters... be compatible with your experimental system. Leakiness If you’re expressing a gene that may be toxic,...inducible promoters are also known for their low leakiness. Inducibility level How much do you want to induce... inducible promoters can vary a lot. If you’re looking for high inducibility, the Tet-On system may be...

  2. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...responsibilities. After my second postdoc, I realized that working in the wet lab wasn’t the right path for me. I ...style of a new, junior team member who had values, working styles, and skills that were quite different from...from them. I started at my previous company by taking on responsibilities such as mentoring a junior ...Beyond Academia: Stories and Strategies for PhDs Making the Leap to Industry." He successfully transitioned...Resources  Resources for aspiring managers Books: The Making of a Manager by Julie Zhuo Radical Candor by Kim...

  3. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...can leave them at room temperature when you’re working with them, but avoid freeze-thaw cycles, as they...bind to amine groups present in some conjugates, blocking the conjugation reaction. Many commercial antibodies...for your ELISA and Western, I’d highly recommend sticking with what works.  To balance this out, however...in your machine before you make your purchase. Picking a single fluorophore for an experiment is easy,...accurate read and reduce needed compensation.    Working with antibodies can get a bit complicated, but ...

  4. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ... in Your Working Day" video from Science Media Savvy At this point, you might be be thinking social media...first blog post out of boredom. I had just started working in a new lab and I was the only person in the lab...first post so I signed up to write a second. While working on this post, I was offered and accepted a position...on the Addgene blog Read about online social networking for scientists Learn why scientists should be...

  5. The time and cost required to make a plasmid

    Type
    Blog Post
    ...digest/ligate methods and picking the right one can save you time if you’re working at speed or money if budget...you can explore to maximize the use of both when making plasmids. Use repositories Repositories such as...free. Plasmids ship out 2-3 days after ordering, making it a toss-up as to whether it’s faster to order...experiment. Make your work “high-throughput” Instead of making one plasmid at a time, try constructing several...

  6. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ... unique cutting features and evolved PAM sites, making Cas9 an adaptable choice for many genome engineering...same mechanism as traditional spCas9. If you are looking to modify RNA, Cas9 just won’t cut it – literally...editing. Fast facts – PAM requirement: protospacer flanking sequence – A, U, or C. Best for: targeting single...use. Fast facts – PAM requirement: protospacer flanking sequence – none. Best for: targeting single stranded...single base pair edits, these enzymes are worth taking a look at. Choosing the right Cas can really put...

  7. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ... the mice to increase their nose-poking behavior. Strong nose-poking behavior is exactly what was seen...a channelrhodopsin, such as cell localization, kinetics, and spectral properties, must be optimized simultaneously...photocurrent strength, wavelength sensitivity, and off-kinetics.   This model was then used to predict which channelrhodopsins...photocurrent amplitude, wavelength sensitivity, and off-kinetics correlated with the model’s predictions.  Three...

  8. RNA Extraction Without A Kit

    Type
    Blog Post
    ...on everything, including your skin. Here are some general tips for working with RNA, even if you are using...basics of isolating RNA without a kit. Tips for working with RNA (whether you are using a kit or not) Although..., 2006).  View our protocol for RNA extraction! Making the simple protocol even more foolproof (still ...still without a kit!) As mentioned above, working with RNA requires keeping your samples cold until homogenization...

  9. Developing Lab Management Software for Biology

    Type
    Blog Post
    ... it’s monumentally difficult without software. Tracking lab procedures also gives you access to hard data...repeated. We also now have a good interface to begin tracking analytics data for lab procedures. With this data...are distinct from their contents. You should be tracking both, you should easily be able to move contents...less time getting up and running and more time working on interesting biotechnology code.There are valid...reasons to choose a non-Python stack, but if you're working with scientists it's unlikely they will have exposure...

  10. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ... There are currently 17 sites around the USA undertaking BEST programs, including local schools in Boston...contributing to the NIH’s Biomedical Research Workforce Working Group. Along with her participation in this panel...Pubmed Central PMCID: PMC3787295. 6. Marder, Eve. "Looking out for future scientists." eLife 3 (2014): e04901...PMC4185419. 7. NIH 2012. Biomedical research workforce working group report. http://acd.od.nih.gov/biomedical_...in Science in Our Career Posts! Get Advice on Networking at Scientific Meetings from Addgene Executive...

  11. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...edit and homology to the genomic DNA locus. After nicking by Cas9, the homologous pegRNA sequence hybridizes..., as it can edit near to or far from PAM sites, making it less constrained by PAM availability than other...previously used when they developed base editing. By nicking the non-edited strand, they can cause the cell ...efficiencies 2-3-fold. However, the process of double nicking increases indel formation slightly. Designing the...the edited strand, as in the PE3b system, guides nicking of the unedited strand only after editing has occurred... ✓ ✓ ✓   ✓ Second sgRNA to guide nicking of the unedited strand Favor the edited strand...

  12. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...understand the risks of the specific virus you’re working with before you work with it. This will help you...required in BSL-1 and required in BSL-2 labs. When working with lentivirus, many organizations require or ...can quickly be removed and disposed of without risking exposure. You can request disposable gowns from...researchers choose to wear eye protection when they are working in a biosafety cabinet, even if it is not required...used for BSL-2 waste, and of course, if you are working with a substance in a biosafety cabinet, it needs...

  13. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    ...include a lot more. Font size: Making your poster readable While we are talking about font, remember this:...important to reflect on these impressions when thinking about crafting your own poster. What sets a scientific...that it will display your data most prominently, making it the star of your presentation. Portrait. This...presented some standard formatting guidelines for making a scientific poster, you shouldn’t hesitate to ...

  14. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...with the NPA where I gained invaluable experience working on and later chairing the Advocacy Committee. I... I’m now furthering my leadership experience working at the Association for Research in Vision and Ophthalmology...website. By volunteering, you can gain experience working with other people mostly online and on monthly ...resulted in actionable tasks and I made a point of sticking to deadlines and performing the work I agreed ...members changed, I had to learn how to develop new working relationships with these individuals who often ...

  15. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ... months have in store for us. If you’re thinking about making the switch from academia to entrepreneurship...entrepreneurs. To make things more interesting, we are working in a new technical discipline that nobody understands...few jobs available. So, we spent a lot of time talking about what we could do to make ourselves marketable...marketable to the wider world and occasionally thinking about how we could bypass the establishment hiring...

  16. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ... they become confluent, taking both early- and late-arising clones, and making sure we always maintain...densities - adding to the expense of dilution cloning. Taking a page from bacterial cloning, we reasoned that...cloning rings. A picture of a 60 mm dish ready for picking is shown in figure 1.   Find Additional Useful ...firmer 2% agarose gel solution discussed above for picking. Many thanks to our guest blogger Iris Lindberg...

  17. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...Additional opportunities: Tracking intracellular pathogens In addition to tracking the location of proteins...University of Queensland) to observe membrane trafficking events in vivo. Locate your protein and see who...your images using subjective judgement by simply “looking at them”. Modern cameras and image analysis software...limited by the wavelength of light and, practically speaking, for common lab microscopes the detection limit...

  18. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...alleles. Although CRISPR is very good at making knockouts, knocking in large sections of DNA can be more ...negative selection marker like the HSV thymidine kinase (HSV-tk) is included just outside one of the homology...” the resistance gene through the insertion of flanking LoxP sequences, you can remove the gene through...described in the preceding sections are simple ways of knocking out a non-essential gene of interest. However,...

  19. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...You’ve spent days and weeks thinking of an amazing project. You’ve written your protocols, designed your...enzymes laying around the lab. If not, you’ll be looking at about $50-$60 per enzyme. Once you’ve got a ... of the restriction enzymes and overall you’re looking at a price of a few dollars per reaction for traditional...relatively new to the cloning game, however, or simply looking for a more streamlined approach, you might want...inserts: There are some cloning methods that make working with multiple inserts a breeze. Among them are ...

  20. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...broken down into different types, with Cas3 systems making up type I, the most abundant type in the Class ...consistent expression can still be a challenge. A multitasking Cas Most Cas effectors have one job — Cas see...away at both the non-target and target strands by making cuts every so often. Cutting both strands ensures...carried out much like the name implies — after nicking, Cas3 reels in the non-target strand of DNA much... for Cas3’s biggest trick. Cas3 likes to bluff, making you into think it’s more powerful than it is. The...
Showing: 361 - 380 of 933 results