Plasmids 101: Common Lab E. coli Strains
Type
Blog Post
...hsdR17(rK- mK+)
XL10 Gold
Tetracycline and Chloramphenicol
High competency cloning and propagation of ...
We narrowed to 387 results for: LOR
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CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...nar/gkw159 Williams RM, Senanayake U, Artibani M, Taylor G, Wells D, Ahmed AA, Sauka-Spengler T (2017). ... -
Viral Vectors 101: Optogenetic Tools
TypeBlog Post...optogenetic tool developed for bidirectional dual-color control of neurons. Nature Communications, 12(1)... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post..., to produce a series of FP-RLuc8 fusions for multicolor imaging. In all of these fusions coelenterazine... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog Post...PBAD), and the A118 gene downstream of the diacetylphloroglucinol (DAPG)-inducible promoter (PPhlF). Output... -
Important Considerations When Using AAVs
TypeBlog Post...171-182. PubMed PMID: 17328683. 11. Wouterlood, Floris G., et al. "A fourth generation of neuroanatomical... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...the haystack” type of problem and led to me to explore alternate approaches. Being a “lazy” geneticist... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog Post...SMJ.0b013e3182498ad5 Curran-Everett, Douglas. Explorations in statistics: statistical facets of reproducibility... -
Plasmids 101: Using Transposons in the Lab
TypeBlog Post...first discovered transposons due to changes in coloration of corn kernels. Aside from corn, transposons... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog Post...for Designing Primers UCSC Genome Browser: For eploring genomic DNA ImageJ: Free Software for Analyzing... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post...https://doi.org/10.1021/acssynbio.3c00167. Hahn, Florian, et al. “A Modular Cloning Toolkit for Genome Editing... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post... by 750 nm to 760 nm laser light, enabling dual color imaging studies with blue or cyan proteins without... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post... Degorce, François, et al. "HTRF: a technology tailored for drug discovery–a review of theoretical aspects... -
Selecting Your Plasmid Purification Kit
TypeBlog Post...kits are quick, easy, and don’t involve phenol-chloroform. The default option is to use the kit that ... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...apyrimidinic sites and abbreviated as AP sites. To explore this further, the team started engineering a CBE... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...below. Figure 2: gRNA target sequences (colored rectangles) are cloned into various plasmids using... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...need such precise measurements, you can use a colorimetric ladder, visible to the naked eye; a fluorescent... -
Optogenetics Guide
TypeGuide...Channelrhodospin plasmids . Halorhodopsins Halorhodopsins are light-gated inward chloride pumps isolated from ...species include: CsChR (from Chloromonas subdivisa ), CoChR (from Chloromonas oogama ), and SdChR (from ...Jaws Browse Halorhodopsin plasmids . Archaerhodopsins Archaerhodopsin-3 (Arch) from Halorubrum sodomense...Channelrhodopsin from Chloromonas oogama CsChR Channelrhodopsin from Chloromonas subdivisa CheRiff Channelrhodopsin... mutations 540 Channelrhodopsins: chloride channels iChloC Chloride-conducting channel, CrChR2 with mutations... ReaChR variant 517 Halorhodopsins Jaws Red-shifted, light-driven inward chloride pump from Haloarcula...identified in other species - by acting as light-gated chloride channels, these variants result in the hyperpolarization... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...intravenous delivery by directed evolution of underexplored sites on the AAV capsid. Molecular Therapy -... -
Antibody Guide
TypeGuide... Here, HRP is used as a colorimetric, in which the reaction produces a colored product that accumulates...well. In colorimetric assays, protein amount can be determined by correlating the amount of color in a well...small multiplex experiments 4-color flow panel: BV421, FITC, PE, and APC 4-color microscopy panel: DAPI for...molecules , proteins that emit a measurable light or color in response to a specific stimulus, can be conjugated...than HRP, fluorophores are available in a range of colors activated by different wavelengths, allowing for...indirect or direct method. Since IF uses fluorophore color to differentiate between the targets of interest...theoretically allow for roughly fifty different fluorophore colors in flow cytometry, but the largest panels used ...