...relevant metadata and the screen results. Screenshots updated 2024. Importantly, metadata for each...to screen result interpretation. The analytical method for score assignment and significance thresholds...gene hits for each screen are also captured as metadata.   Screen results are reported as the original...

...that was developed by Li, et al., in 2011. This method is ligation independent, it does not require purification...and then combine the products and digest with methylation sensitive restriction enzyme DpnI to remove the... Note—templates must have been isolated from methylation competent E. coli for this procedure to work ..., sequence-and ligation-independent PCR cloning method." BMC biotechnology 11.1 (2011): 1. PubMed PMID...

...suitable for flow cytometry and fluorescence microscopy, they also show that this method is compatible with...University has developed a new CRISPR screening method that makes use of guide RNAs with internal barcodes...the majority of cells receive one gRNA. The iBAR method resulted in high quality data even when used at...

... substrates inside the lysosome. Historically, methods for studying autophagy focused on counting the ...Daniel J., Ana Maria Cuervo, and Per O. Seglen. "Methods for monitoring autophagy from yeast to human." ..., Noboru, Tamotsu Yoshimori, and Beth Levine. "Methods in mammalian autophagy research." Cell 140.3 (2010...quantitative Pho8Δ60 assay of nonspecific autophagy." Methods in enzymology 451 (2008): 33-42. PubMed PMID: 19185711...

...through our hot plasmids articles. Think we missed something smokin'? You can pitch a Hot Plasmids section ...of our most commonly requested antibodies. But sometimes, one antibody per target just isn’t enough and...agents. Adapted from Fig. 2 Campbell et al, Nature Methods   Find YFP plasmids here!   Campbell, B.C.,...fluorescent proteins for advanced microscopy. Nat Methods 19, 1612–1621 (2022). https://doi.org/10.1038/s41592...

...your tables and figures. Now it's time to write. Sometimes, it's hard to get those words out and onto the..., you can take a longer 15-30 minute break. The method is named for the tomato or “Pomodoro” kitchen timer...manuscript they might be found, i.e. introduction, methods, results; or phrases are categorized by the goal...This podcast talks more about using the Pomodoro method in grad school Additional resources on the Addgene...

... or good - they're just exist and sometimes we benefit and sometimes we don't."     - Jennifer Tsang, ...age) fight in the Napoleonic Wars and against something even more sinister. 1000 pages long. Incredibly...International Finance. A book club could lead to smart, sometimes contentious, discussions. I presented the first...

... a high throughput manner. She helped develop a method that measures the effects of sequence variants ... the body’s response to warfarin. “These novel methods will be instrumental in interpreting human variation...sensor can also kinetically outperform current methods. Unger plans to use the sensor to answer the question...Pantoea agglomerans interaction. But the shape and metabolism of leaves can be changed by light and temperature...

...taken up the plasmid in the same way. Selection methods, described below, are typically employed when creating...direct test for your gene of interest may be another method to assess transfection success. GFP is often used... a problem depending on the duration or other parameters of your experiment. Mammalian cells double at..., but still want to use transient transfection methods. Since there are no "natural" mammalian ORIs, scientists...

..., starvation or the use of caloric restriction mimetics trigger autophagy and, in a mouse model led to...anti-cancer immunosurveillance and reduced tumor mass. Methods to study autophagy can be found here. Fun fact:...immunotherapy is the combination of the CRISPR/Cas9 methodology with T cell receptor engineering (Ref 5)  as ...Pietrocola, Federico, et al. "Caloric restriction mimetics enhance anticancer immunosurveillance." Cancer...

...sophisticated methods of sex and gender analysis. Universities need to incorporate these methods into their...Innovations. This project: 1) develops state-of-the-art methods of sex and gender analysis and 2) provides case...For more practical help, there are numerous methodological articles to consider—we have loaded those we...

...explained in more detail here. There are a variety of methods that can be used to measure your fluorescent protein...complicating the interpretation. Because of this, these methods only give a qualitative view of dimerization tendency...the protein forms dimers. Finally, spectroscopic methods (based on fluorescence polarization) can be used...homodimerization. However, all the aforementioned methods only determine the capacity of homodimerization...

... remind you of anything? Perhaps the Scientific Method? Imagine your problem is just a question you have... skills” is the ability to apply the scientific method to problems in a variety of contexts.  How do you...skills? Thankfully, as scientists, the scientific method is a core part of our academic training.  Developing...their logic. Or you can try applying the scientific method to everyday problems in your life. This tactic ...

...is sifting through the long list of titles for something that is actually useful to you. When embarking... the jargon and background in your field, but sometimes papers are also just poorly written. By nature... or are trying to replicate an experiment, the methods section probably won’t get much attention reading...of the appropriate controls? Refer back to the methods section for finer points of the protocols. Are ...

...repeats separated by an 8-bp long asymmetric core spacer sequence. The asymmetry in the core sequence gives ...specified times. Combining this with some of the loxP methods described above, a genetic modification can be ...site have been created, which contain a unique asymmetric spacer "NNNTANNN", where "N" indicates which ...

...more than one protein in your sample? Or what if something weird happened during your western and your results...antibodies, especially with more stringent stripping methods. If you are planning to detect multiple proteins... most PVDF membranes recommend re-wetting with methanol before rinsing with wash buffer, as dry PVDF won... that has been dried, no matter what stripping methods you throw at it. Ideally, dry your membrane once...

... tool in the field as it provides a noninvasive method for manipulating cell activity, especially neuronal... a chemogenetic system, you’ll need to choose a method for targeting receptor expression as a ligand and...expression must be restricted to specific circuits. Methods for targeting expression involve tissue specific...as a DREADD agonist due to its ability to be metabolized into clozapine (CLZ). While some scientists argue...

...beginning. Variability source Standardization parameters Animal Strain, age, sex, housing conditions...reduce expression levels of certain genes. A popular method uses Cre recombinase on transgenic flox animals... area of interest may be very limited with this method, especially in adult animals, and not targeted ...order to maximize possible effects. Injection Parameters: The injection needle should be as thin as possible...

...pieces of the FP back together (Figure 1). This method is generally known as Bimolecular Fluorescence ...GFP reassembly is a fast, efficient, and robust method for detecting protein-protein interactions in vivo...options, check out the 2in1 Kit for FRET and ratiometric BiFC from Christopher Grefen’s lab or explore...Remember that the FP fragments themselves can sometimes interact in cells and produce background signal...

...the laser and measuring its output with a light meter before and after polishing. Efficiency of the implant...series already provided or check the materials and methods of a paper that uses the variant you wish to use... pulses. An example of a standard stimulation parameter is 20Hz (laser output), 5ms “on”, 50ms “off”.  ...lasers are also available. Optimal stimulation parameters must also be determined empirically but it’s ...