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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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Showing: 361 - 380 of 416 results

  2. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...transfection. Test a variety of brands and FBS lot numbers to find one suitable with your protocols. FBS can...-Tip Transducing too many cells relative to the number of virus particles reduces the transduction efficiency...massive cell death upon antibiotic selection. The number of cells transduced should be enough that they ...amount of time, but not so many that they vastly outnumber the virus particles. Incubate the cells with the...lentivirus used, cells may also exhibit varying numbers of integration events. In other words, if an MOI...

  3. Guide to Using Pooled Libraries

    Type
    Guide
    ... the number of plasmids present in the library - for example, the Moffat lab uses 200x the number of cells...cells as plasmids in the TKO library. Using large numbers of cells minimizes the effects of random chance...

  4. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ... is challenging to monitor because only a small number of glutamate molecules are released in each synaptic...

  18. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...to insert ratio of approximately 1:3. Since the number of base pairs for each varies, it is difficult ...the more common chemically-competent cells. The number of bacterial colonies resulting from your transformation... plate that are not correct. If you have a high number of colonies on your recipient plasmid alone plate... ligations. Pick 3-10 colonies depending on the number of background colonies on your control plate (the...

  19. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...100uM stock of any primer, add a number of µl of dH 2 O equal to the number of nanomoles of DNA times 10....by creating a “master mix,” which minimizes the number of small volumes you need to pipet. If you are ...Preparation Multiply the volume of each reagent by the number of individual PCRs you wish to perform and add ...

  20. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...to insert ratio of approximately 1:3. Since the number of base pairs for each varies, it is difficult ...the more common chemically-competent cells. The number of bacterial colonies resulting from your transformation... plate that are not correct. If you have a high number of colonies on your recipient plasmid alone plate... ligations. Pick 3-10 colonies depending on the number of background colonies on your control plate (the...
Showing: 361 - 380 of 416 results