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...addition, it increases avidity because it doubles the number of binding sites per unit.  There are several ways...

...versions have been developed, which differ in the number, stability, and brightness of the fluorescent proteins...

... methods for studying protein phosphorylation A number of classic methods exist for this, the most common...

...in situ hybridization for detection of gene copy number changes." Protocols for Nucleic Acid Analysis by...

...excited. This approach may also be applicable to a number of rare genetic diseases caused by splicing defects...

...problem is that the discussions usually focus on the numbers, rather than the necessary resources. This is why...

...is expressed in the hippocampus, or counting the number of puncta in each cell. When making figures for...

... is challenging to monitor because only a small number of glutamate molecules are released in each synaptic...

...treatment.  Westerns are also a useful readout for a number of cellular assays. They can determine protein ...

...loci is possible using CRISPR, and Addgene has a number of tools for this purpose. Epigenetics began as...

...Biology is messy enough that by chance, some small number of the scientists will get a result that confirms...

... repressible binary systems. In recent years, a number of more complex systems have also been developed...

...would for a Western blot in order to reduce the number of cells that will bind non-specifically to either...

...target states are only represented in a very small number of registers. Instead, one may use qPCR to determine...

...to insert ratio of approximately 1:3. Since the number of base pairs for each varies, it is difficult ...the more common chemically-competent cells. The number of bacterial colonies resulting from your transformation... plate that are not correct. If you have a high number of colonies on your recipient plasmid alone plate... ligations. Pick 3-10 colonies depending on the number of background colonies on your control plate (the...

...100uM stock of any primer, add a number of µl of dH 2 O equal to the number of nanomoles of DNA times 10....by creating a “master mix,” which minimizes the number of small volumes you need to pipet. If you are ...Preparation Multiply the volume of each reagent by the number of individual PCRs you wish to perform and add ...

...to insert ratio of approximately 1:3. Since the number of base pairs for each varies, it is difficult ...the more common chemically-competent cells. The number of bacterial colonies resulting from your transformation... plate that are not correct. If you have a high number of colonies on your recipient plasmid alone plate... ligations. Pick 3-10 colonies depending on the number of background colonies on your control plate (the...

...prediction is ever perfect, but this can decrease the number of guides one needs to test in the lab. Importantly...

...communication options. Addgene, along with an increasing number of academic labs, uses Slack constantly. Whether...

...FACS dosing method correlates viral titre to the number of cells transduced by a GFP encoded virus, and...