We narrowed to 651 results for: PPL

1. Adapting Toehold Switches to Detect Zika Virus

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   ...focused on developing cell-free synthetic biology applications for human health and the environment.     Alexander...nanomaterials science, and self-assembly for applications in health, energy, and sustainability.   References...

2. Cpf1 Update: Comparison to Cas9 and NgAgo

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   ...eclipse any of the CRISPR nucleases in AAV-based applications. Get More Cpf1 Background Here THE ORIGINAL ...the PAM sequence. Many research and clinical applications require HDR over NHEJ, so comparing HDR efficiency...

3. The Materials Science of Optogenetics Experiments

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   ...the initial laser output. The same considerations apply to the polishing and preparation of the patch cable...Injectable, cellular-scale optoelectronics with applications for wireless optogenetics. Science. 2013;340...

4. Experimenting in an Open Source lab: from CRISPR to Cats

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   ... in biology but don’t really know how to start applying their skills to it. The Kittybiome project gives...contributes each month - with only occasional supplementation with grant funds, money is always tight. Of...

5. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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   ...and respond to novel substances. We both had an applied science bent, so the engineering aspect of this...about continuing with the business. We ended up applying to a business accelerator freely stating that ...

6. Production of Virus in Insect Versus Mammalian Cells

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   ...something you may have to optimize for your specific application. With mammalian systems, it is standard to do...titer the virus, or go straight into your viral application, like protein production. Titration of baculovirus...

7. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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   ...machine learning (PRIDICT, PRIDICT2.0).   How to apply PRIDICT in prime editing experiments Prediction ... computational biology. His expertise includes applying machine learning techniques to make sense of biology...

8. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   ...and quiescent, post-mitotic mammalian cells. Applications of CRISPR delivery by adenoviral vectors Since...Strategies for Tissue-Specific Targeting. In: Applications of viruses for cancer therapy. Elsevier, pp ...

9. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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   ...resources are limited or for bioart and teaching applications. Find a chromoprotein for your experiment! ...Scarless Modification of Proteobacterial Genomes. Applied and Environmental Microbiology, 85(16). https:/...

10. Google Forums Round Up: First Impressions of NgAgo

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    ...been excitedly testing NgAgo's genome editing applications. So, how does NgAgo stack up thus far? A few... S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for genome engineering." Cell...

11. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    ...problem for fusion proteins. One of the major applications of FPs is to visualize the localization, dynamics...An experimental study of GFP-based FRET, with application to intrinsically unstructured proteins. Protein...

12. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    ...species in question), why not use them whenever applicable?Against this, consider the cost of an accidental...An accidental release could delay real-world applications against scourges such as malaria and dengue ...

13. Great Results Start with Great Standard Curves

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    ... critical for a number of popular scientific applications, and the quality of a standard curve can make...for an acceptable R2 but for many scientific applications users aim for an R2 above 0.95.    Additional...

14. Getting to Know Expansion Microscopy

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    ...with an abundance of protocols and examples of applications from across disciplines now available. In this...Tissue expansion is not compatible with in vivo applications and there’s just not much to be done about that...

15. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ... and have been adapted for a wide variety of applications - as mutagenesis tools for genetic studies (...at one or multiple desired genomic loci. Many applications of these systems have already been demonstrated...

16. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...PIPs, resulting in a fluorescent readout.   Applications for bimolecular fluorescence complementation...resulting from the assayed PPI. This high-throughput application is much more straightforward to carry out because...

17. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    ... primarily on fluorescent proteins and their applications, tackles much broader issues in this brief volume...successful scientists have outgoing personalities to supplement their intellectual prowess and scientific skills...

18. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    ...is a Ph.D. candidate at MIT. His research uses applied machine learning and statistical methods for fundamental...fundamental scientific discovery and high-impact applications.       Alvin Hsu is a graduate student at Harvard...

19. A Guide to Starting Your Own Journal Club

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    ...notice typically should include the paper, any supplemental data, software repositories, or other information....  These variations are suited for different applications and should be carefully chosen for each group...

20. An Introduction to Adenovirus

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    ...Persistent infection by adenoviruses. J Clin Pathol Suppl (R Coll Pathol)., 6, 39. https://doi.org/10.1136...on the Addgene blog Viral Vectors 101: Viral Applications Adenoviral Vector Production and Troubleshooting...